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A Developmental study of Islet Isolation Method and Immunological Modification on Islet Cells

Research Project

Project/Area Number 04807092
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Digestive surgery
Research InstitutionChiba University

Principal Investigator

ENOMOTO Kazuo  Chiba University SURGETY(II), ASSISTANT, 医学部・附属病院, 助手 (70223659)

Co-Investigator(Kenkyū-buntansha) JINGU Kazuhiko  Chiba University SURGETY(II), CLINICAL FELLOW, 医学部・附属病院, 医員
ARITA Seiji  Chiba University SURGETY(II), CLINICAL FELLOW, 医学部・附属病院, 医員
KENMOCHI Takashi  Chiba University SURGETY(II), CLINICAL FELLOW, 医学部・附属病院, 医員
ASANO Takashi  Chiba University SURGETY(II), LECTURER, 医学部, 講師 (80143311)
小林 進  千葉大学, 医学部, 助手 (50234828)
Project Period (FY) 1992 – 1993
Project Status Completed (Fiscal Year 1993)
Budget Amount *help
¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1993: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1992: ¥1,200,000 (Direct Cost: ¥1,200,000)
KeywordsMechanical Chopping / Automated Digestion Method / Islet Isolation / Islet Number / Islet Volume / Pancreas Transplantation / 膵移植 / インシュリン依存性糖尿病 / I型糖尿病 / ランゲルハンス島移植 / ラ代島単離法 / 自動コラゲナーゼ持続潅流ラ代島分離装置 / メカニカルチッツパー / コラゲナーゼ
Research Abstract

In order to solve the limittation of sufficient islets yield, we combined our original mechanical chopping apparatus before using Ricordi thpe automated digestion apparatus, and experimentally studied the efficacy of this method in canie models. After injection of HBSS containing collagenase (Type S-1, Nitta Co, Osaka) to the pancreas duct, panceata were, first, divided to two groups, one was used for islet isolation by an sutomated digestion system (ADS) only, and the other was first chopped by a mechanical chopper (MC) then digested by the ADS.By the time of chopping, the later group was divided to three as 5 sevond, 10 sec and 15 sec group.
The pancreata that were used for the combined method were chopped into 2-5 mm fragments in a few second by the MC without islet disorders. Duration of the digestion Phase was significatly shorter compared with the result of the ADS only group. the MC combined method, especially in 5 sec group, allowed the higher islet yield. an average of 2602 islets/g pancreas with an estimated average volume of 3.68 mul/g pancreas, than the yield form the method of the ADS only. The study of size distribution of the purified islets showed that both islet diameter range and islet number por gram of pancreas were larger in the MC combined group. and this tendency was clear in larger islet diameter range. Islet vvolume per gram of pncreas was also larger in the MC cobined method. MC could proceed the choppong prosess without traumatic action to the islets. The combined method of MC and leads to incrase the islet yield.
It is suggested form this canine study that the combined method might ve one of the effective mathod for islet isolation i humans also.

Report

(3 results)
  • 1993 Annual Research Report   Final Research Report Summary
  • 1992 Annual Research Report
  • Research Products

    (10 results)

All Other

All Publications (10 results)

  • [Publications] Takashi Kenmochi: "Prediction of viability of ischemically damaged canine Pancreatic grafts by tissue flow rate with machine perfusion." Transplantation. 53. 745-750 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 剣持 敬: "同種膵移植におけるFK506の有効性に関する実験的研究" 日本外科学会雑誌. 93. 626-631 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 浅野 武秀: "臓器保存の現状と展開・新しい臓器保存液" 今日の移植. 6. 257-261 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Takashi Kenmochi, Takehide Asano, Toshio Nakagouri, Kaichi Isono and Hiroshi Horie: "Prediction of viability assay of ischemically damaged canine pancreatic grafts by tissue folw rate with machine perfusion" Transplantation. 53. 745-750 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Takashi Kenmochi, Takehide Asano, Seiji Arita, Toshio Nakagouri, Kazuo Enomoto, Takenori Ochiai and Kaichi Isono: "Efficacy of FK506 on Pancresa Transplantation" Jap J Surgery. 6. 626-631 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Takehide Asano and Seiji Arita: "Present and Future of organ Preservation --new preservation solution--" Jap J Transp. Today. 3. 257-261 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Kazuhiko Jingu, Takehide Asano, Takashi Kenmochi, Kazuo Enomoto, Takeshi uematsu, Toshio Nakagouri, Yoshiharu Tokoro, Yoshifimi Matsui, Michihiro Maruyama, Hideaki Miyauchi and Kaichi Isono: "Combined method of Mechanical Chopper and Automated Digestion System For Islet Isolation" Proceedings of the 4th International Congress of Pancreas and Islet Transpl.(1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Takashi Kenmochi: "Prediction of viability of ischemically damaged canine Pancreatic grofto by tissue flow rate with machine perfusion." Transplantation. 53. 745-740 (1992)

    • Related Report
      1993 Annual Research Report
  • [Publications] 剣持 敬: "同種膵移植におけるFK506の有効性に関する実験的研究" 日本外科学会雑誌. 93. 626-631 (1992)

    • Related Report
      1993 Annual Research Report
  • [Publications] 浅野 武秀: "臓器保存の現況と展開・新しい臓器保存液" 今日の移植. 6. 257-261 (1993)

    • Related Report
      1993 Annual Research Report

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Published: 1992-04-01   Modified: 2016-04-21  

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