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Elucidation of genetic disorders in skeletal dysplasias.

Research Project

Project/Area Number 04807105
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Orthopaedic surgery
Research InstitutionOsaka University

Principal Investigator

NAKAHARA Haruhiko  Osaka Uni.Med.School, Orthopaedics, Assist Professor, 医学部, 助手 (10198179)

Co-Investigator(Kenkyū-buntansha) ONO Keiro  Osaka Uni.Med.School, Orthopaedics, Professor, 医学部, 教授 (70028330)
KIMURA Tomoatsu  Osaka Uni.Med.School, Orthopaedics, Associal Professor, 医学部, 講師 (80167379)
Project Period (FY) 1992 – 1993
Project Status Completed (Fiscal Year 1993)
Budget Amount *help
¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1993: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1992: ¥1,200,000 (Direct Cost: ¥1,200,000)
Keywordsperiosteum / bone / cartilage / differentiation / cytokines
Research Abstract

Periosteum-derived cells have been reported to exhibit osteogenic and chondrogenic potentials in high cell density culture conditions. Using this system, the effects of TGFbeta and BMP-2 on proliferation and differentiation of periosteal mesenchymal cells were studied. TGFbeta shortened the time course of chondrogenesis and increased the amount of cartilage formation. On the other hand, the amount of bone decreased with TGFbeta treatment, whereas the time course of osteogenesis remained unaffected. BMP-2 enhanced bone formation without any effect on chondrogenesis. The developmental potential of periosteum-derived cells was also clonally assessed with an agar gel culture system. Two types of colonies were predominantly observed, one type of colony being chondrogenic, the other type osteogenic colonies. Supplementation with TGFbeta to this system shortened the time course of chondrogenesis and also increased colony forming efficiency of chondrogenic colonies. On the other hand, colony forming efficiency of osteogenic colonies decreased with TGFbeta treatment. These observations suggest that there are both committed osteoprogenitor and chondroprogenitor cells present in the periostel cell population, and TGFbeta stimulates proliferation and differentiation of chondrogenic cell population by its targeted action. These resuls taken together suggest that TGFbeta and BMP regulate osteochondrogenic differrentiation of cells in the periosteum in the process of fracture healing.

Report

(3 results)
  • 1993 Annual Research Report   Final Research Report Summary
  • 1992 Annual Research Report
  • Research Products

    (17 results)

All Other

All Publications (17 results)

  • [Publications] K.Nakata,H.Nakahara,T.Kimura,A.Kojima,M.T.Inoshita,A.Caplan,K.Ono: "Collagin gene expression during dioradrogenesis from drick periostedm-derived cells" FEBS LETTERS. 299. 278-282 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] M.Iwasaki,K.Nakata,H.Nakahara,T.Nakase,T.Kimura,K.Kimata,A.Caplan,K.Ono: "Transforming growth factor-β1 Stinulates Chondnogonsis and inhibits osteogenosis in high density culture of periosteum-derived cells" Endocrinology. 132. 1603-1608 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] T.Nakase,H.Nakahara,M.Iwasaki,T.Kimura,T.Kimata,K.Watanabe,A.Caplan,K.Ono: "Clonal analysis for developmental potential of chick periosfeum derived cells : Agargel" Biochemical and Biophysical Research Communixdisis. 195. 1422-1428 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] M.Iwasaki,T.Nakase,H.Nakahara,K.Takaoka,A.Caplan,K.Ono: "Bone Morphogenetic Protein-2 stimulates osfecgenisis and deopoat affect clrovadrcgcnesis,in osteggiondigcnic olifferentiation of periosteum-derived cells." J.Bore Miner.Res. (in press). (1994〔)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] K.Nakata, H.Nakahara, T.Kimura, A.Kojima, M.Iwasaki, A.Caplan, K.Ono: "Cllager gene expression during drordroosteagenesis from chick periosfecem-derived cells." FEBS LETTERS. 299. 278-282 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] M.Iwasaki, K.Nakata, H.Nakahara, T.Nakase, T.Kimura, K.Kimata, A.Caplan, K.Ono: "Transforming gronth factor-beta1 stimulates chordrogenesis and inpibits osteogenesis in high kensity culfure of periostecmderived cells." Endoorinology. 132. 1603-1608 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] T.Nakase, H.Nakahara, M.Iwasaki, T.Kimura, K.Kimata, K.Watanabe, A.Caplan, K.Ono: "Clonal analysis for developmental potential." Biochonical and Biophysical Research Communications I. 195. 1422-1428 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] M.Iwasaki, T.Nakase, K.Takaoka, A.Caplan, K.Ono: "Bove Morplicgenetic protein-alpha stimulate osteogenesis and doen not affect chordrogenesis in osteodrovdrogenic differentiation of periosteum-derived cells." J.Bone Miner Res. (in press). (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] K.Nakata,H.Nakahara,T.Kimura,A.Kojima,M.Iwasaki,A.Caplan,K.Ono: "Collagen gene expression during chondrogenesis from chick poriosteum-derived cells" FEBS LETTERS. 299. 278-282 (1992)

    • Related Report
      1993 Annual Research Report
  • [Publications] M.Iwasaki,K.Nakata,H.Nakahara,T.Nakase,T.Kimura,K.Kimata,K.Ono: "Transforming growth factor-β1 stimulates chondrogenesis and inhibits osteogenesis in high density culture of periosteum-derived a" Endocrinology. 132. 1603-1608 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] T.Nakase,H.Nakahara,M.Iwasaki,T.Kimura,K.Kimata,K.Watanabe,K.Ono: "Clonal analysis for developmental potential of chick periosteum-derived cell:Agar gel culture system" Biochemical and Biophysical Research Communications. 195. 1422-1428 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] M.Iwasaki,T.Nakase,H.Nakahara,K.Takaoka,A.Caplan,K.Ono: "Bone morphogenetic protein-2 stimulates osteogenesis but does not affect chondrogenesis in osteochondrogenic differentiation of periosteum-derived cells." J.Bore Miner Res. (in press). (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] Iwasaki,M.,Nakata,K.,Nakahara,H.,Nakase,T.,Kimura,T.,Kimata,K.A.I.Caplan Ono,K.: "Transforming Growth Factor-β_1 Stimulates Chondrogenesis and Inlabits Osteogenesis in High Density Culture of Periosteum-Derived Cells." Endocrinology. 132. (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] Iwasaki,M.,Nakata,K.,Nakahara,H.,Nakase,T.,Kimura,T.,Ono,K.: "Transforming Growth Factor-β_1 Stimulates Chondrogenesis in Culture of Periosteal Cells." The 3rd.Conference of The International Society for Fracture Pepair(IsFR-92). 25-26

    • Related Report
      1992 Annual Research Report
  • [Publications] Nakase,T.,Iwasaki,M.,Kimura,T.,Nakata,K.,A.I.Caplan Kimata,K.,Nakahara,H.: "Clonal Analysis for Developmental Potential of Chick Periosteum-Derived Cells-Agar Gel Cultuse System-" Transactions of the 39th Annual Meeting Orthopaedic Research Society 抄録集. 485-

    • Related Report
      1992 Annual Research Report
  • [Publications] Iwasaki,M.,Nakase,T.,Nakahara,H.,Takaoka,K.,A.I.Caplan.,Ono,K.: "Bone Morphogenetic Protein-2 Stimulates Osteogenesis in High Density Culture of Periosteum-Derived Cells" Transactions of the 39th Annual Meeting Orthopaedic Research Society 抄録集. 483-

    • Related Report
      1992 Annual Research Report
  • [Publications] 中原 治彦,Arnold.I.Caplan Victor.M.Goldberg: "培養ヒト骨膜細胞の骨膜骨形成能について多孔性セラミックとの複合移植" 第12回整形外科セラミック・インプラント研究会抄録集.

    • Related Report
      1992 Annual Research Report

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Published: 1992-04-01   Modified: 2016-04-21  

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