| Project/Area Number |
04807106
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Osaka University |
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Principal Investigator |
OCHI Takahiro (1993) Osaka University Environmental Medicine Professor, 医学部, 教授 (80112035)
東 文造 (1992) 大阪大学, 医学部, 助手 (50231915)
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| Co-Investigator(Kenkyū-buntansha) |
KIMURA Tomoatsu Osaka University Orthpaedics Assistant Professor, 医学部, 講師 (80167379)
ONO Keiro Osaka University Orthpaedics Professor, 医学部, 教授 (70028330)
越智 隆弘 大阪大学, 医学部, 教授 (80112035)
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| Project Period (FY) |
1992 – 1993
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| Project Status |
Completed (Fiscal Year 1993)
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| Budget Amount *help |
¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 1993: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1992: ¥1,200,000 (Direct Cost: ¥1,200,000)
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| Keywords | Reumatoid arthritis / Serum / Citotoxity / Inhibitor / 組織傷害 / 細胞傷害性因子 |
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| Research Abstract |
The peripheral blood serum of patients with severe active rheumatoid arthritis (RA) was found to have a cytotoxic effect on autologous mononuclear cells. The cytotoxic factor was partially purified and shown to have a molecular weight of about 900 kd, using gel filtration. The factor differed from cytokines such as tumor necrosis factor-alpha (TNF-alpha) and also differed from complement components. It also differed from hydrolytic enzyme such as protease, collagenase, and phospholipase A_2. It destroyed normal peripheral blood mononuclear cells in vitro at the same concentration as it found in vivo, and also nonspecifically destroyed such human cell lines as CCRF-CEM, Raji, U-937, and HL-60. Each RA patient with this serum cytotoxic factor had severe systemic tissue disease as well as rapid and severe destruction of most joints. Thus, we have identified a cytotoxic factor in RA serum which causes systemic tissue damage. And we also partially pirified the inhibitor against the cytotoxic factor, using gel filtration.
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