| Project/Area Number |
04807109
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Kurume University |
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Principal Investigator |
YAMANAKA Kensuke Kurume Univ.School of Med.Professor, 医学部, 教授 (10080821)
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| Co-Investigator(Kenkyū-buntansha) |
HASHIMOTO Sanshiro Kurume Univ.School of Med.Fellow, 医学部, 助手 (00268913)
YANAGIDA Iwao Kurume Univ.School of Med.Fellow, 医学部, 助手 (50248431)
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| Project Period (FY) |
1992 – 1994
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| Project Status |
Completed (Fiscal Year 1994)
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| Budget Amount *help |
¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1994: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1993: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1992: ¥600,000 (Direct Cost: ¥600,000)
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| Keywords | Rheumatoid Arthritis (RA) / Substance P (SP) / Substance P / Subustance P / Preprotachykinin / in situ hybridization法 |
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| Research Abstract |
It is known that substance P (SP) plays an important role as immunological mediator in inflammation and the substance also acts as signal transmission in neuronal system. It could therefoere be assumed that SP may involve in the pathogenesis of rheumatoid arthritis (RA).
The aim of this study was to demonstrate the presence of SP in synovial fluid and the distribution on synovial membrane of RA.The concentration of SP in the synovial fluid was found significantly higher in the RA group than in the osteoarthritis (OA) group. The level of SP in the rheumatoid synovial fluid was correlated with the severity of inflammation. At least two possibilities as source of SP in the synovial fluid were considered. SP could be supplied by nerve endings of sensory neurons which normally distributed in the synovial membrane, and the other is synthesized and released from immune cells infiltrating the synovium. In the present study, preprotachykinin (PPT) mRNA,which code for SP and related peptides was expressed in the rheumatoid synovium using in situ hybridization histochmistry, while the PPT mRNA was not detected in the synovium of OA.
These results revealed that SP in the rheumatoid joint may play an important role in the pathogenesis and pathophisiology of RA.
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