| Project/Area Number |
04807150
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
小児・社会系歯学
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| Research Institution | Niigata University |
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Principal Investigator |
NODA Tadashi Niigata Univ.Sch.of Dentistry, Professor, 歯学部, 教授 (00013970)
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| Co-Investigator(Kenkyū-buntansha) |
SATO Takuichi JSPS,JSPS Research Fellow, 特別研究員
OHSHIMA Kuniko Niigata Univ.Sch.of Dentistry, Assistant Professor, 歯学部, 助手 (80213693)
ISHIKURA Yuuka Niigata Univ.Dental Hospital, Lecturer, 歯学部・附属病院, 講師 (60232279)
HOSHINO Etsuro Niigata Univ.Sch.of Dentistry, Professor, 歯学部, 教授 (90124619)
佐藤 択一 日本学術振興会, 特別研究員
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| Project Period (FY) |
1992 – 1994
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| Project Status |
Completed (Fiscal Year 1994)
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| Budget Amount *help |
¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1994: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1993: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1992: ¥1,000,000 (Direct Cost: ¥1,000,000)
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| Keywords | Children / Eubacterium / Deciduous teeth / Infected root canals / Obligate anaerobes / Oral microflora |
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| Research Abstract |
The purpose of this study was to investigate the bacterial composition of necrotic pulps of human deciduous teeth by sampling the split surfaces of freshly extracted teeth and culturing the bacteria present with good anaerobic isolation techniques. Significantly more bacteria were recovered after the incubation in an anaerobic chamber than after aerobic incubation in air with 30 per cent CO_2. Of 276 bacterial isolates, 251 (91 per cent) were obligate anaerobes. These findings suggest that the environment of necrotic pulps in human deciduous teeth is anaerobic and thus favors the growth of anaerobes. Among the 251 obligate anaerobes isolated, strains belonging to the genera Peptostreptococcus (25 per cent), Propionibacterium (19 per cent), Eubacterium (17 per cent) and Fusobacterium (13 per cent) were major parts of the bacterial flora of the lesions of human deciduous teeth. Bifidobacterium (2 per cent), Lactobacillus (1 per cent), Actinomyces (1 per cent) and Veillonella (0.7 per cent) were minor parts of the flora. The microflora of necrotic pulps of human deciduous teeth is in some respects similar to that reported for the deep layrs of dentinal lesions of adults.
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