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Bacteriological and Immunological study on endodontic lesions of human deciduous teeth

Research Project

Project/Area Number 04807150
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 小児・社会系歯学
Research InstitutionNiigata University

Principal Investigator

NODA Tadashi  Niigata Univ.Sch.of Dentistry, Professor, 歯学部, 教授 (00013970)

Co-Investigator(Kenkyū-buntansha) SATO Takuichi  JSPS,JSPS Research Fellow, 特別研究員
OHSHIMA Kuniko  Niigata Univ.Sch.of Dentistry, Assistant Professor, 歯学部, 助手 (80213693)
ISHIKURA Yuuka  Niigata Univ.Dental Hospital, Lecturer, 歯学部・附属病院, 講師 (60232279)
HOSHINO Etsuro  Niigata Univ.Sch.of Dentistry, Professor, 歯学部, 教授 (90124619)
佐藤 択一  日本学術振興会, 特別研究員
Project Period (FY) 1992 – 1994
Project Status Completed (Fiscal Year 1994)
Budget Amount *help
¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1994: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1993: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1992: ¥1,000,000 (Direct Cost: ¥1,000,000)
KeywordsChildren / Eubacterium / Deciduous teeth / Infected root canals / Obligate anaerobes / Oral microflora
Research Abstract

The purpose of this study was to investigate the bacterial composition of necrotic pulps of human deciduous teeth by sampling the split surfaces of freshly extracted teeth and culturing the bacteria present with good anaerobic isolation techniques. Significantly more bacteria were recovered after the incubation in an anaerobic chamber than after aerobic incubation in air with 30 per cent CO_2. Of 276 bacterial isolates, 251 (91 per cent) were obligate anaerobes. These findings suggest that the environment of necrotic pulps in human deciduous teeth is anaerobic and thus favors the growth of anaerobes. Among the 251 obligate anaerobes isolated, strains belonging to the genera Peptostreptococcus (25 per cent), Propionibacterium (19 per cent), Eubacterium (17 per cent) and Fusobacterium (13 per cent) were major parts of the bacterial flora of the lesions of human deciduous teeth. Bifidobacterium (2 per cent), Lactobacillus (1 per cent), Actinomyces (1 per cent) and Veillonella (0.7 per cent) were minor parts of the flora. The microflora of necrotic pulps of human deciduous teeth is in some respects similar to that reported for the deep layrs of dentinal lesions of adults.

Report

(4 results)
  • 1994 Annual Research Report   Final Research Report Summary
  • 1993 Annual Research Report
  • 1992 Annual Research Report
  • Research Products

    (17 results)

All Other

All Publications (17 results)

  • [Publications] Sato Takuichi: "Predominant obligate anaerobes in necrotic pulps of human deciduous teeth." Microbial Ecology in Health and Disease. 6. 269-275 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Sato Takuichi: "In vitro antimicrobial susceptibility to combinations of drugs of bacteria from carious and endodontic lesions of human deciduous teeth." Oral Microbiology and Immunology. 8. 172-176 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Sato Takuichi: "Bactericidal efficacy of a mixture of ciprofloxacin,metronidazole,minocycline and rifampicin against bacteria of carious and endodontic lesions of human deciduous teeth in vitro." Microbial Ecology in Health and Disease. 5. 171-177 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 上松弘幸: "歯周ポケット内細菌叢の栄養学的特徴" 新潟歯学会雑誌. 22. 145-146 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 星野悦郎: "口腔Eubacterium" 新潟歯学会雑誌. 22. 1-14 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 星野悦郎: "パ-シャルデンチャーの細菌学(I)(II) In:パ-シャルデンチャーをみる目" 日本歯科評論・別冊 水谷紘,星野悦郎(編)(印刷中), (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Sato Takuichi: "Predominant obligate anaerobes in necrotic pulps of human deciduous teeth." Microbial Ecology in Health and Disease. No.6. 269-275 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Sato Takuichi: "In vitro antimicrobial susceptibility to combinations of drugs of bacteria from carious and endodontic lesions of human deciduous teeth." Oral Microbiology and Immunology. No.8. 172-176 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Sato Takuichi: "Bactericidal efficacy of a mixture of ciprofloxacin, metronidazole, minocycline and rifampicin against bacteria of carious and endodontic lesions of human deciduous teeth in vitro." Microbial Ecology in Health and Disease. No.5. 171-177 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Sato Takuichi: "Predominant obligate anaerobes in necrotic pulps of human deciduous teeth." Microbial Ecology in Health andDisease. 6. 269-275 (1993)

    • Related Report
      1994 Annual Research Report
  • [Publications] Sato Takuichi: "In vitro antimicrobial susceptibility to combinations of drugs of bacteria from carious and endodontic lesions of human deciduous teeth." Oral Microbiology and Immunology. 8. 172-176 (1993)

    • Related Report
      1994 Annual Research Report
  • [Publications] Sato Takuichi: "Bactericidal efficacy of a mixture of ciprofloxacin,metronidazole,minocycline and rifampicin against bacteria of carious and endodontic lesions of human deciduous teeth in vitro." Microbial Ecology in Health and Disease. 5. 171-177 (1992)

    • Related Report
      1994 Annual Research Report
  • [Publications] 上松弘幸: "歯周ポケット内細菌叢の栄養学的特徴" 新潟歯学会雑誌. 22. 145-146 (1992)

    • Related Report
      1994 Annual Research Report
  • [Publications] 星野悦郎: "口腔Eubacterium" 新潟歯学会雑誌. 22. 1-14 (1992)

    • Related Report
      1994 Annual Research Report
  • [Publications] 星野悦郎: "パ-シャルデンチャーの細菌学(I)(II) In:パ-シャルデンチャーをみる目" 日本歯科評論・別冊 水谷 紘,星野悦郎(編)(印刷中), (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] Sato Takuichi: "Predominant obligate anaerobes in necrotic pulps of human deciduous teeth." Microbial Ecology in Health and Disease. 6. 269-275 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Sato Takuichi: "In vitro antimicrobial susceptibility to combinations of drugs of bacteria from carious and endodontic lesions of human deciduous teeth." Oral Microbiology and Immunology. 8. 172-176 (1993)

    • Related Report
      1993 Annual Research Report

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Published: 1992-04-01   Modified: 2016-04-21  

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