| Project/Area Number |
04807169
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Laboratory medicine
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| Research Institution | Fukushima Medical College |
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Principal Investigator |
YOSHIDA Hiroshi professor., 医学部・検査部, 教授 (90045683)
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| Co-Investigator(Kenkyū-buntansha) |
OHARA Morihiro associate prof., 医学部・内科, 講師 (60128543)
IMAFUKU Yuji associate, 医学部・検査部, 助手 (40254015)
NAGAI Toshihiko associate, 医学部・検査部, 助手 (90180447)
MIURA Yutaka associate prof., 医学部・検査部, 助教授 (50045647)
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| Project Period (FY) |
1992 – 1993
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| Project Status |
Completed (Fiscal Year 1993)
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| Budget Amount *help |
¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1993: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1992: ¥900,000 (Direct Cost: ¥900,000)
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| Keywords | endothelin endothelin / ALP / LDH / RA / synovial A type cell / joint lesion / 関節滑膜 / A型様細胞 / ALP / 関節滑膜細胞 |
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| Research Abstract |
Cultured type A(like) synoviocytes obtained from RA patients at corrective surgery were shown to secrete endothelin(ET). alkalin phosphatase(ALP) and lactic dehydrogenase(LDH). The mechanisms of their productions and properties of the enzymes were investigated.
ET1 was secreted from A type synoviocytes, but not from B type cells. The maximum concentration in culture fluid was observed at 24 h after renewal of the fluid. Analyzes by RHPLC revealed that the major ET was ET1 and minor components including big ET1 were also detected. ET concentrations in synovial fluids did not differ between RA and OA patients.
ALP secreted from A type cells was suggested to be a new subtype, based on the following properties ; distributed in alpha_1-beta region on electrophoresis, neuraminidase sensitive, heat-stable, slighty inhibited by l-phenylalanine and high affinity to wheat germ lectin(>90%). Subtypes of LDH were LDH4 and LDH5, suggesting that A type cell is the major source of LDH in the synovium.
The significance of ET, ALP and LDT in the pathogenesis of joint inflammation is unknown and further investigation is absolutely necessary.
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