| Project/Area Number |
04833003
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
分子細胞生物学
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| Research Institution | The University of Tokyo |
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Principal Investigator |
SAKO Yasushi The University of Tokyo Assist. Prof.College of Arts and Sciences, 教養学部, 助手 (20215700)
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| Co-Investigator(Kenkyū-buntansha) |
KUSUMI Akihro The University of Tokyo Assoc.Prof.College of Arts and Sciences, 教養学部, 助教授 (50169992)
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| Project Period (FY) |
1992 – 1993
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| Project Status |
Completed (Fiscal Year 1993)
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| Budget Amount *help |
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1993: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1992: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Keywords | plasma membrane / membrane proteins / transiferrin receptor / coated pitt / adoptor complex / single particle tracking / optical twtezems / cytoskoleton / 一粒子追路法 / 膜ドメイン / 側方拡散 / 膜骨格 / レーザー光トラップ |
|---|
| Research Abstract |
Movements of transferrin receptor molecules in the plasma membrane of cultured normal rat kidney fibroblastic cells were investigated by video-enhanced contrast optical microscopy with nanometer-level special precision and video-rate temporal resolution by lebeling the receptor with the ligand-coated 40-nm colloidal gold particles. Most of the movement trajectories are of the confined diffusion type within domains of -0.25mum^2. Movement within the domains is random with a diffusion coefficient -10^<-9> cm^2/s, which is consistent with that expected for free Brownian diffusion of proteins in the plasma membrane. The receptor molecules move from one domain to one of the adjacent domains at an average frequency of 0.034s^<-1>, indicating that the plasma membrane is compartmentalized for diffusion of membrane receptors and that long-range diffusion is the result of successive intercompartmental jumps. Partial destruction of the cytoskeleton suggest that the boundaries between compartments are made of dynamically fluctuating membrane skeletons.
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