| Project/Area Number |
04833025
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
分子細胞生物学
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| Research Institution | Kansai Medical University |
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Principal Investigator |
MATSUBARA Hiroaki Kansai Medical University, Assistant Professor, 医学部, 助手 (10239072)
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| Project Period (FY) |
1992 – 1993
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| Project Status |
Completed (Fiscal Year 1993)
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| Budget Amount *help |
¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1993: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1992: ¥1,200,000 (Direct Cost: ¥1,200,000)
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| Keywords | Thyroid hormone / atrial natriuretic factor / gene expression / atrial myocyte / cardiac myocyte / 心房性ナトリウム利尿ホルモン |
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| Research Abstract |
Atrial natriuretic factor(ANF) is a hormone that dilates vasculature and induces natriuresis, and is synthesized and secreted from atrial myocytes. We have demonstrated for the first time that thyroid hormone(T_3)stimulates synthesis and release of ANF from atrial myocytes, and that its stimulatory effect is mediated through gene transcription. To characterize the T_3 response element(TRE)of ANF promoter, we deletion-mutated 2.4Kb of the 5' flanking region toward the downstream and fucked 5' end of chrolamphenicol acetyltransferase(CAT)gene. These deletion-mutated CAT constructs were transfected into the atrial myocytes. We identified the TRE in the region located in -108 to -68bp. The gel shift assay demonstrates that this region binds to the nuclear factor induced by the addition of T_3 and no retarded band was observed in the nuclear extract in the absence of T_3. No consensus TRE motif previously recognized was detected in -108 to -68. Thus, we characterized the TRE specific for ANF gene in -108 to -68 from the transcription site, the sequence of which conferred the T_3 responsiveness to the ANF gene.
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