| Budget Amount *help |
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1993: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1992: ¥1,000,000 (Direct Cost: ¥1,000,000)
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| Research Abstract |
The purpose of the research is to analyze mechanisms of intracellular-transportation and localization of small molecular weight GTP binding proteins, Fro this purpose, we have been using fission yeast, Schizosaccharomyces pombe, as a model system. In the first year of the research, we purified pombe rasl protein. The biochemical analyzes of the protein revealed that the protein, unlike other ras proteins, has larger Kd value to GDP than to GTP.This is the first report of the biochemical analyzes of pombe rasl protein. We also isolated a new gene that encoded for novel G-protein coupled receptor protein. Knowing the characteristics of the rasl protein, we moved to reconstruct posttranslational modification system in vitro in the second, and it is the last year of the project. From the kinetic analyzes of the partially purified farnesyl transferase, we found out the difference in the rate constant rather than Km value against various peptide substrates. This will be further investigated to clarify the mechanism of substrate selection by various preny1-transferases in vivo.
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