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Bone reconstruction by osteoblasts conjugated implants in aged human

Research Project

Project/Area Number 04836001
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 老化(加齢)
Research InstitutionHOKKAIDO UNIVERSITY

Principal Investigator

MIZUNO Morimichi  HOKKAIDO UNIV., SCHOOL OF DENTISTRY, INSTRUCTOR, 歯学部, 助手 (10125354)

Co-Investigator(Kenkyū-buntansha) KUBOKI Yosinori  HOKKAIDO UNIV., SCHOOL OF DENTISTRY, PROFESSOR, 歯学部, 教授 (00014001)
Project Period (FY) 1992 – 1993
Project Status Completed (Fiscal Year 1993)
Budget Amount *help
¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1993: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1992: ¥1,100,000 (Direct Cost: ¥1,100,000)
KeywordsBone marrow cells / Osteoblasts / Collagen / Differentiation / Calcifiaction / Hydroxyapatite / コラーゲンゲル / コラーゲン ゲル
Research Abstract

(1) Bone marrow cells contained stem cells that possess the potency of multi-differentiation. These cells differentiate into fibrolasts when they were cultured on conventional culture dishes.
When they were cultured on collagen gel, they differentiated into osteoblasts and formed calcified nodules.
(2) The calcified nodules were investigated by electron- micro-scopic observation, and matrix vesicle-like elements were recognized. Furthemore, needle-like crystals were precipitated on collagen fibers, In these crystals, calcium and phosphate was identified by x-ray microanalyzer, and neelde-like crystals were suspected to be hydroxyapatite.
(3) These findings indicated that the process of mineralization in calcified nodules is similar to that of body, and the calcified nodules formed by bone marrow cells are same with bone.
(4) The inductive activity of collagen is specific for type I collagen and the gel. If type I collagen was dried and bone marrow cells were cultured on membrane-type collagen, collagen did not show inductive activity against bone marrow cells.
(5) These results indicate that features of collagen is crucial for the inductive activity against bone marrow cells.

Report

(3 results)
  • 1993 Annual Research Report   Final Research Report Summary
  • 1992 Annual Research Report
  • Research Products

    (13 results)

All Other

All Publications (13 results)

  • [Publications] Sasano.Y: "BMPs induce direct bone formation in ectopic sites independent in vivo and in vitro" The Anatomical Record. 236. 373-380 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Kuboki.Y: "Hydroxyapatite as a supporter for osteoblasts differentiation in vivo and in vitro" Apatite. 1. 311-320 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Nagai.N: "Histological studies of heteotopic osteogenesis induced by bone morphogenetic protein binding apatite" Apatite. 1. 321-330 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Sasano, Y.Mizuno, M.and Kuboki, Y.: "BMPs induce direct bone formation in ectopic sites independent in vivo and in vitro" The Anatomical Records. 236. 373-380 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Kuboki, Y.Murata, T.Mizuno, M.and Nagai, Y.: "hydroxyapatite as a carrier for osteoblasts differentiation in vivo and in vitro" Apatite. 1. 311-320 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Nagai, Y.Inoue, N.Mizuno, M.and Kuboki, Y.: "Histological studies of heterotopic osteogenesis induced by bone morphogenetic protein binding apatite" Apatite. 1. 321-330 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Sasano.Y: "BMPs induce direct bone formation in eotopic sites independent of the endochondral ossification in vivo" The Anatomical Record. 236. 373-380 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Kuboki Y: "Hydroxyapatite as a supporter for osteoblasts differentiation in vivo and in vitro" Apatite. 1. 311-320 (1992)

    • Related Report
      1993 Annual Research Report
  • [Publications] Nagai N: "Histological studies of heteotopic osteogenesis induced by bone morphogenetic protein binding hydroxyapatite" Apatite. 1. 321-330 (1992)

    • Related Report
      1993 Annual Research Report
  • [Publications] Mizuno.M..: "An Osteonectin-Like Protein in the Matrix of Cultured Osteogenic Cell-Line MC3T3-E1" Calcified Tissue International. 51. 156-161 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Zhou H-Y: "Effect of a bone lysine-rich 18 KDa protein on osteoblaet-like MC3T3-E1 Cells" Biochem.Biophys.Res.Commun.186. 1288-1293 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 水野 守道: "骨芽細胞の分化におけるOsteocalcin,OsteonectinそしてOsteopontin遺伝子の発現について" 日本骨代謝学会誌. 10. 21-29 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Mizuno M.: "Time-dependent expression of the osteopontin gene in osteogenic cell-line MC3T3-E1" Connective Tissue Research. 27. 132- (1992)

    • Related Report
      1992 Annual Research Report

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Published: 1992-04-01   Modified: 2016-04-21  

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