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Studies on the gene expression of OSF-1 in osteoblasts derived from normal and osteoporosis patients.

Research Project

Project/Area Number 04836021
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 老化(加齢)
Research InstitutionKyoto Pref.Univ.of Medicine

Principal Investigator

HASHIMOTO-GOTOH Tamotsu  Kyoto Pref.Univ.Med.Associated Prof., 医学部, 助教授 (00237942)

Co-Investigator(Kenkyū-buntansha) TSUJIMURA Atsushi  Kyoto Pref.Univ.Med.Assistant Prof., 医学部, 助手 (50236890)
OOKUMA Seitaro  Kyoto Pref.Univ.Med.Associated Prof., 医学部, 助教授 (30152086)
HIRASAWA Taisuke  Kyoto Pref.Univ.Med.Prof., 医学部, 教授 (40079851)
Project Period (FY) 1992 – 1994
Project Status Completed (Fiscal Year 1994)
Budget Amount *help
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1994: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1993: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1992: ¥1,200,000 (Direct Cost: ¥1,200,000)
KeywordsOSTEOBLASTS / OSTEOPOROSIS / NEURO TROPIC FACTOR / DEVELOPMENT / 遺伝子発現 / Xenopos laevis / development / 骨芽細胞
Research Abstract

OSF-1 has the activities promoting the differentiation and cell growth on osteoblasts and neurite out growth on neurons. The amino acid sequences are highly conserved among mammals' (human, bovine, mouse, rat) OSF-1, so the functions are seemed to have important and fundamental rolls on the osteogenesis and the nerve development. To study the functions of OSF-1 on the metabolism of bone, the relationship between the osf-1 expression and osteoporosis was examined. The nucleotide sequence of osf-1 cDNA derived from human osteoblasts was cofirmed to identical to that expressed in brain tissues. Next, the amount of osf-1 expression was compared between osteoblasts derived from osteoporosis patients and from healthy individuals. As the results, the expression of type I collagen was somewhat high in osteoblasts derived from patients, but the osf-1 and osteopontine expression levels were almost the same with non-patients samples. Then transgenic mice which over express human osf-1 gene under the regulation of bone specific promoter was constructed. Until now the phenotype of the transgenic mice were normal comparing to the non-transgenic mice. We are planing to construct a mice which defect the osf-1 gene expression by disruption of the gene by the targeting method.
We also focused on the fact that osf-1 is highly expressed during development. Other osteo specific factors such as BMPs are functioned in both osteogenesis and embryo genesis. To investigated the osf-1 function on the embryo genesis we isolated Xenopus laevis osf-1 homologue cDNA clones. The expression of x-osf-1 was increased at the tail-bud stage and the expression site was limited at the hind brain region. So osf-1 was thought to be a regulatory factor to develop a specialized tissues rather than the funcamental level such as BMPs which concerning in the determination of the axis of the embryo.

Report

(4 results)
  • 1994 Annual Research Report   Final Research Report Summary
  • 1993 Annual Research Report
  • 1992 Annual Research Report
  • Research Products

    (18 results)

All Other

All Publications (18 results)

  • [Publications] 橋本保 他: "骨粗鬆症患者と健常者由来骨芽細胞における特異的遺伝子発現の解析とcDNAクローニング" 臨床成人病. 23. 1614-1615 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] M.Okazaki 他: "Molecular cloning and characterization of OB-cadherin, a new member of cadherin family expressed in osteoblasts" J.Biol Chem. 269. 12092-12098 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 橋本保 他: "Differential hybridization スクリーニング法を用いた組織特異的に発現する遺伝子のクローニングと解析" 日本薬理学雑誌. 102. 47-52 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 手塚建一 他: "骨芽細胞特異的に発現している因子(OSF-1)のクローニングとその解析" 日本骨代謝誌. 10. 14-20 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] T.Hashimoto-Gotoh: "An oligodeoxyribonocleotide-directed dual amber method for site directed mutagenesis" Gene. 152. 271-275 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] T.Hashimoto-Gotoh: "Construction and characterization of new host-vector system for the enforcement-cloning" Gene. 137. 211-216 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Toba, M., Minowa: "Characterization of spontaneous elimination of streptomycin sensitivity gene on high copy number plasmids : Sms-enforcement cloning vectors with synthetic rpsL gene." Gene. 121. 25-33 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Tsuji, H.et.al.: "Hereditary ATIII deficiency : Identification of an Arg406 to Met point mutation near protease reactive site." In Molecular Aproaches to he Study and Treatment of Human Diseases (New York, USA : Elsevier Science). 51-55 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] T.Hashimoto-G et.al.: "Construction and characterization of new host-vector systems for the enforcement-cloning method." Gene. 137. 211-216 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] T.Hashimoto-G et.al.: "An oligodeoxyribonucleotide-directed dual amber method for site-directed mutagenesis." Gene. 152-2. 271-275 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Tada, N.et.al.: "Isolation and characterization of a mouse Protein C cDNA." J.Biochem.111. 491-495 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Katsura, M., et.al.: "Nicotine increases diazepam binding inhibitor (DBI) mRNA in primary cultured" Neuroscience Letters. 168, neurons.1-4 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Okazaki, M., et.al.: "Molecular cloning and characterization of OB-cadherin, a new member of cadherin family expressed in osteoblasts." J.Biol.Chem.269. 12092-12098 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] T.Hashimoto Gotoh 他: "An Oligodeoxyribonucleotide-directed doal amber method for Site directed mutagenesis" Gene. 152. 271-275 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] M.Okazaki 他: "Molecular cloning and characterization of OB-Cadherin,a new menber of cadherin family expressed in osteoblasts." J.Biol.Chem.269. 12092-12098 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 橋本保: "骨粗鬆症患者と健常者由来骨芽細胞における特異的遺伝子発現の解析とcDNAクローニング" 臨床成人病. 23. 1614-1615 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Mari Toba-Minowa: "Characterization of the spontaneous elimination of steptomycin sensitivity(Sm^S)on high-copy-number plasmid:Sm^S-enforcement cloning vectors with a synthetic rpslgene" Gene. 121. 25-33 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Tamotsu Hashimoto-Gotoh: "Current Perspective on Molecular & Cellolar Oncolosy Volume1.PartA^<211>〜_<230> Unosual Sequene conservation in Intron-1 of c-H-ras Oncogene and Its effect on p21 Protein synthesis" JAI Press Ltd, 20 (1992)

    • Related Report
      1992 Annual Research Report

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Published: 1992-04-01   Modified: 2016-04-21  

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