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A study on the mechanism of decreased osteo genic capacity with aging by rat bone marrow cells

Research Project

Project/Area Number 04836022
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 老化(加齢)
Research InstitutionNara Medical University

Principal Investigator

DOHI Yoshiko  Medical School Nara Medical University, Associate Prof, 医学部, 助教授 (50155628)

Co-Investigator(Kenkyū-buntansha) TABATA Shiro  Medical School Nara Medical University, Professor, 医学部, 教授 (40041652)
OHGUSHI Hajime  Medical School Nara Medical University, Research Associate, 医学部, 助手 (80213669)
森山 忠重  奈良県立医科大学, 医学部, 教授 (60075041)
Project Period (FY) 1992 – 1994
Project Status Completed (Fiscal Year 1994)
Budget Amount *help
¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1994: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1993: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1992: ¥800,000 (Direct Cost: ¥800,000)
KeywordsOsteoblast / Ectopic osteogenesis / Aging / Bone marrow / Bone matrix proteins / Osteocalcin / Osteopontin / Differentiation / Osteogenesis(骨形成) / 加齢 / 骨髄 / Diffusion Chamber / オステオカルシン / 化骨形成 / 骨基質蛋白質
Research Abstract

It is presently unclear whether osteoporosis is due to increased bone resorption or to decreased bone formation. A major difficulty has been the scarcity of animal models available for examining the effect of age on bone formation and resorption. We have demonstrated the ability of bone marrow cells to differentiate to form cartilage and bonewithin a diffusion chamber (J.Bone Miner.Res.7 : 1173-1180,1992). Using the diffusion chamber implantation method, we analyzed the bone formation capacity of 8-, 12- and 23-week-old rat bone marrow cells. Measurements of Ca, phosphorus and bone Gla protein (BGP) contents in the DC implants with different aged rat bone marrow clearly demonstrates that bone formation decrease dramatically with over 23-week-old rat bone marrow. Northern blot analysis showed either osteopontin (OP) or BGP mRNAs was expressed intensely in the DC implants with young rat bone marrow. In the DC implants with older rat bone marrow OP mRNA could be detected weakly, whereas BGP mRNA could not.
On the other hand, in vitro subculture of both young and aged rat bone marrow cells showed mineralized nodules and showed the osteoblastic phenotype expression. However, DNA content and alkaline phosphatase (ALP) activity in cultured old marrow cells were slightly lower than those in cultured young marrow cells. These results indicate that osteogenic differentiation of bone marrow declines with age.
And dexamethasone, a differentiation-inducing reagent, seems to be crucial for the osteoblastic differentiation for rat cells, especially old marrow cells.

Report

(4 results)
  • 1994 Annual Research Report   Final Research Report Summary
  • 1993 Annual Research Report
  • 1992 Annual Research Report
  • Research Products

    (14 results)

All Other

All Publications (14 results)

  • [Publications] Takafuumi Yoshikawa: "Biochemical and histological sequences of membraneous ossification in ectopic site" Calcified Tissue International. 50. 184-188 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Yoshiko Dohi: "Osteogenesis associated with bone Gla protein gene expression in diffusion chambers by bone marrow cells with demineralized bone matrix" Journal of Bone and Mineral Research. 7. 1173-1180 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Yoshiko Dohi: "Effect of cadmium on osteogenesis within diffusion chambers by bone marrow cells : Biochemical evidence of decreased bone formation capacity" Toxicology and Applied Pharmacology. 120. 274-280 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kanya Honoki: "Correlation between lack of bone Gla protein mRNA expression in rat transplantable osteosarcomas and expression of both c-fos and c-jun proto-oncogenes" Molecular Carcinogenesis. 7. 111-115 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Hajime Ohgushi: "Osteogenic defferentiation of marrow stromal stem cells in porous hydroxyapatite caramics" Journal of Biomedical Meterials Research. 27. 1401-1407 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Hajime Ohgushi: "Tissue compatibility of biomaterials : assessment of bioactivity concerning the osteogenic response to the materials" Materials Science and Engineering. C1. 139-142 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] T.Yoshikawa, H.Ohgushi, M.Okumura, S.Tamai, Y.Dohi and T.Moriyama: "Biochemical and histological sequences of membraneous ossification in ectopic site." Calcif.Tissue Int. 50. 184-188 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Y.Dohi, H.Ohgushi, S.Tabata, T.Yoshikawa, K.Dohi and T.Moriyama: "Osteogenesis associated with bone Gla-protein gene expression in diffusion chambers by bone marrow cells with demineralized bone matrix" J.Bone Miner.Res. 7. 1173-1180 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Y.Dohi, K.Sugimoto, T.Yoshikawa, H.Ohgushi, T.Katsuda, S.Tabata and T.Moriyama: "Effect of cadmium on osteogenesis within diffusion chambers by bone marrow cells : Biochemical evidence of decreased bone formation capacity." Toxicol.Appl.Pharmacol. 120. 274-280 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] K.Honoki, Y.Dohi, S.Tabata, Y.Mii, Y.Miyauchi, M.Tsutsumi and Y.Konishi: "Correlation between lack of bone Gla-protein mRNA expression in rat transplantable osteosarcomas and expression of both c-fos and c-jun oncogene." Mol.Careinog. 7. 111-115 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] H.Ohgushi, Y.Dohi, S.Tamai and S.Tabata: "Osteogenic differentiation of marrow stromal stem cells in porous hydroxyapatite ceramics" J.Biomed.Mat.Ros. 27. 1401-1407 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] H.Ohgushi, M.Okumura, K.Inoue, S.Tamai, S.Tabata and Y.Dohi: "Tissue compatibility of biomaterials : assessment of bioactivity concerning the osteogenic response to the materials" Mat.Sci.Engineer. C1. 139-142 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Y.Dohi,K.Sugimoto et al: "Effect of cadmium on osteogenesis within diffusion chambers by bone marrow cells." Toxicol.Appl.Pharmacol.120. 274-280 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 井上恵介、大串始、土肥祥子他4名: "骨形成に対する老化の影響(骨髄細胞の骨芽細胞への分化に対する生化学的および組織学的検索)" 日本整形外科学会誌. 67. S1253- (1993)

    • Related Report
      1993 Annual Research Report

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Published: 1992-04-01   Modified: 2016-04-21  

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