• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Study on Synaptic Plasticity in Aging Brain Tokyo Metropolitan Inst. Gerontology,

Research Project

Project/Area Number 04836028
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 老化(加齢)
Research InstitutionTokyo Metropolitan Institute of Gerontology

Principal Investigator

ANDO Susumu  Dept. of Membrane Biochemistry, Research Director, 生体膜部門, 研究部長 (30073000)

Project Period (FY) 1992 – 1993
Project Status Completed (Fiscal Year 1993)
Budget Amount *help
¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1993: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1992: ¥1,200,000 (Direct Cost: ¥1,200,000)
KeywordsAging / Brain / Synapse / Plasticity / Na / K-ATPase / Cholinergic / Ganglioside
Research Abstract

Dysfunction of aging brains is speculated to be accelerated by neuronal loss or diminished density of synapses. Three objectives of this study were as follows : 1. to examine the age-related changes of synaptic functions ; 3. to test whether the prevention of the decrement of synaptic density might retard the decreasing brain functions.
We found significant decreases in resting membrane potential and in the activity of an electrogenic enzyme, Na/K-ATPase as a synaptic dysfunction in a previous work (Brain Res., 506 : 46-52, 1989). In this study, the cntent of Na/K-ATPase in synaptic membranes was proved to decrease in late senescence. Secondly, four new species of ganglioides were identified as neuronal membrane markers. Two of them were assigned as cholinergic neuron-specific antigens, Chol-1, and named GT1aalpha and GQ1balpha. Othe two were O-acetylated gangliosides, O-Ac-GT3 and O-Ac-GT2. The latter two were shown to be localized on the growth cone membranes of dendritic fibers. Thirdly, rat brains were demonstrated to greatly develop in rearing under enriched environment. Their cognitive functions such as memory and learning ability were better improved during maturation than control, and were maintained on a level with young stage during senescence. Quntitation of a synaptic marker protein, synaptophysin revealed that the synaptic density definitely increasedin enriched environmental group than in control. This may indicate the possibility that brain dysfunction due to aging could be prevented or retarded by increasing density of synapses.

Report

(3 results)
  • 1993 Annual Research Report   Final Research Report Summary
  • 1992 Annual Research Report
  • Research Products

    (23 results)

All Other

All Publications (23 results)

  • [Publications] Waki,H.,Murata,A.,Kon,K.,Maruyama,K.,Kimura,S.,et al.: "Isolation and characterization of a trisialyl-lactosylceramide,GT3,containing an O-acetylated sialic acid in cod fish brain." J.Biochem.113. 502-507 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Nakao,T.,Kon,K.,Ando,S.,Miyatake,T.,Yuki,N.,et al.: "Novel lacto-ganglio type gangliosides with G_<M2>-epitope in bovine brain which react with IgM from a patient of the amyotrophic lateral scleros." J.Biol.Chem.268. 21028-21034 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Waki,H.,Masuzawa,A.,Kon,K.& Ando,S.: "A new O-acetylated trisialoganglioside,9-O-acety-GT2,in cod brain." J.Biochem.114. 459-462 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Igarashi,M.,Waki,H.,Saito,S.,Komiya,Y.& Ando,S.: "Characteristics of gangliosides including O-acetylated species,in growth cone membranes at several developmental stages in rat forebrain." Dev.Brain Res.(in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Hidari,K.I.-P.Jwa,Irie,F.,Suzuki,M.,Kon,K.,et al.: "A novel ganglioside with a free amino group in bovine brain." Biochem.J.296. 259-263 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Saito,S.,Kobayashi,S.,Ohashi,Y.,Igarashi,M.,et al.: "Decreased synaptic density in aged brains and its prevention by rearing under enriched environment as revealed by synaptophysin contents." J.Neurosci.Res.(in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Waki, H., Murata, A., kon, K., Maruyama, K., Kimura, S., Ogura, H.& Ando, s.: ""Isolation and characterizatin of a trisialyllactosylceramide, GT3, containing an O-acetylated sialic acid in cod fish brain."" J.Biochem.113. 502-507 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Nakao, T., kon, K., Ando, S., Miyatake, T., Yuki, N., Li, Y.-T., Furuya, S.& Hirabayashi, Y.: ""Novel lacto-ganglio type gangliosides with G_<M2>- epitope in bovine brain which react with IgM from a patient of the amyotrophic lateral scleros is-like disorder."" J.Biol. Chem.268. 21028-21034 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Waki, H., Masuzawa, A., Kon, K.& Ando, S: ""A new O-acetylated trisialoganglioside, 9-0-acetyl GT2, in cod brain."" J.Biochem.114. 459-462 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Igarashi, M., Waki, H., Saito, S., Komiya, Y.& Ando, S: ""Characteristics of gangliosides including O-acetylated species, in growth cone membranes at several developmental stages in rat forebrain."" Dec.Brain Res.(in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Hidari, K.I.-P.Jwa, Irie, F., Suzuki, M., Kon.K.Zndo, S.& Hirabayashi, Y.: ""A novel ganglioside with a free amino group in bovine brain."" Biochem.J.296. 259-263 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Saito, S., Kobayashi, S., Ohashi, Y., Igarashi, M., Komiya, Y.& Ando, S.: ""Decreased synaptic density in aged brains and its prevention by rearing under enriched environment as revealed by synaptophysin contents."" J.Neurosci.Res.(in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Waki,H.,Murata,A.,Kon,K.,Maruyama,K.,et al.: "Isolation and characterization of a trisialyllactosylceramide,GT3,containing an O-acetylated sialic acid in cod fish brain." J.Biochem.113. 502-507 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Nakao,T.,Kon,K.,Ando,S.,Miyatake,T.,et al.: "Novel lacto-ganglio type gangliosides with GM2-epitope in bovine brain which react with IgM from a patient of the amyotrophic." J.Biol.chem.268. 21028-21034 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Waki,H.,Masuzawa,A.,Kon,K.& Ando,S.: "A new O-acetylated trisialoganglioside,9-O-acetyl GT2,in cod brain" J.Biochem.114. 459-462 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Igarashi,M.,Waki,H.,Saito,S.,et al.: "Characteristics of gangliosides in cluding O-acetylated species,in growth cone membranes at several developmental stages." Dev.Brain Res.(in press).

    • Related Report
      1993 Annual Research Report
  • [Publications] Hidari,K.I.-P.Jwa,Irie,F.,Suzuki,M.,et al.: "A novel ganglioside with a free amino group in bovine brain." Biochem.J.296. 259-263 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Saito,S.,Kobayashi,S.,Ohashi,Y.,et al.: "Decreased synaptic density in aged brains and its prevention by rearing under enriched environment as revealed." J.Neurosci.Res.(in press).

    • Related Report
      1993 Annual Research Report
  • [Publications] Ando,S.,Hirabayashi,Y.Kon,K.,Inagaki,F.,Tate,S.&Wittaker,V.: "A trisialoganglioside containing a sialyl α-2-6 N-acetylgalactosamine residue is a cholinergic-specific antigen,Chol-l." J.Biochem.,. 111. 287-290 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Tanaka,Y.&Ando,S.: "Age-related changes in 〔^3H〕 ouabain binding to synaptic plasma membranes isolated from mouse brains." J.Biochem.,. 112. 117-121 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Hirabayashi,Y.,Nakao,T.,Irie,F.,Wittaker,V.P.&Ando,: "Structural characterisation of a novel cholinergic-specific ganglioside in bovine brain." J.Biol.Chem.,. 267. 12973-12978 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 安藤 進,脇 初枝: "C系列ガングリオシドと神経分化" 蛋白質・核酸・酵素. 37. 1908-1913 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 安藤 進: "「新老年学」(編集代表:荒茂肇)1章3.3 生体膜の老化" 12 (1992)

    • Related Report
      1992 Annual Research Report

URL: 

Published: 1992-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi