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Identification of a new species of sea snake

Research Project

Project/Area Number 05041104
Research Category

Grant-in-Aid for international Scientific Research

Allocation TypeSingle-year Grants
SectionField Research
Research InstitutionSophia University

Principal Investigator

TAMIYA Toru  Sophia University, Associate Prof., 理工学部, 助教授 (30119135)

Co-Investigator(Kenkyū-buntansha) ZIMMERMAN Keneth  University of Newcastle, Lecturer, 理学部, 講師
HEATWOLE Harold  North Carolina State Universty, Prof., 理学部, 教授
COGGER Harold  Australian Museum, Director, 副館長
HARVEY Alan  ストラスクライド大学(スコットランド), 薬学部, 教授
BOULAIN Jean  サクレー原子力研究所(仏国), タンパク工学, 主任研究員
MENEZ Andre  CEN Saclay, Director, タンパク工学, 部長
TORIBA Michihisa  Japan Snake Institute, Researcher, 主任研究員 (40109856)
TAMIYA Nobuo  Tohoku University, Honorable Prof., 理学部, 東北大学名誉教授 (90004225)
ENDO Toshiya  Nagoya University, Prof., 理学部, 教授 (70152014)
HARRY Alan L.  University of Strathclyde, Prof.
BOULAIN Jean-Claude  CEN Saclay, Researcher
HEATWOLE Har  米国ノースカロライナ大学, 動物学, 教授
HARVEY Alan.  英国ストラスクライド大学, 薬学部, 教授
HEATWDE Haro  ノースカロライナ大学, (米国)・理学部, 教授
Project Period (FY) 1993 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥13,000,000 (Direct Cost: ¥13,000,000)
Fiscal Year 1995: ¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 1994: ¥5,000,000 (Direct Cost: ¥5,000,000)
Fiscal Year 1993: ¥5,000,000 (Direct Cost: ¥5,000,000)
Keywordssea snakes / neurotoxin / polymorphism / Laticauda / colubrina / laticaudata / frontalis / semifasciata / semifasciata
Research Abstract

To identify the new spices of sea snake Laticauda frontalis, field research has been undertaken in Fiji, Tonga, Vanuatu and New Caledonia. The snake L.frontalis is almost identical to Laticauda colubrina. This species is apparently restricted to the islands of Vanuatu except for a specimen collected a century ago on one of New Caledonianls Loyalty Islands.
The project is part of a broader study of variation in venomous sea snakes throughout the Pacific region. The research being undertaken by our group is of two pricipal kinds : studies of the venom of these snakes and broader studies into their taxonomy and ecology.
To data, the Vanuatu species Laticauda frontalis is known only from various sites on Efate and Espritu Santo, and it will be important in future work to attempt to determine the geographic limits of this species, together with the extent of morphological and genetic variation in Vanuatu. Deduced amino acid sequences of neurotoxins from the venom gland cDNAs of L.frontalis are different from that of L.colubrina from various countries except from New Caledonia. Deduced amino acid sequences of L.frontalis are similar to that of New Caledonian L.colubina. Midbody scales of L.colubrina from Fiji and Vanuatu are 23 to 25. However, midbody scales of L.frontalis from Vanuatu and L.colubrina from New Caledonia are 21 to 23.
We concluded that species Laticauda frontalis should add as a new species in genus Laticauda. Laticauda colubrina in New Caledonia should classify as subspecies of Laticauda frontalis.

Report

(3 results)
  • 1995 Final Research Report Summary
  • 1994 Annual Research Report
  • 1993 Annual Research Report
  • Research Products

    (23 results)

All Other

All Publications (23 results)

  • [Publications] Tremeau O.,etal.: "Genetic engineering of snake toxins" J.Biol.Chem.270. 9362-9369 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Segalas I.,et al.: "Sotution strueture of a green mamba toxin that activate muscarinic acetylcholin receptors." Biochemistry. 34. 1248-1260 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Harvey AL.,et al.: "Structure-activity studies on scorpion toxins that block potassium channels" Toxicon. 33. 425-436 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Maillere B.,et al.: "Immunogenicity of a disulphidecontaining neurotoxin:Presentation to T-cells requires a reduetion step" Toxicon. 33. 475-482 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Dauplais M.,et al.: "An NMR study of the intuaction of cardiotoxin gamma fum Naja nigricoh with predenatured do decylphos phochdin micelles" Eur.J.Biochem. 230. 213-220 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Vita C.,et al.: "Scorpion toxins as natural scaffdds for protein engineering" Proc.Natl.Acad.Sci.U.S.A. 92. 6404-6408 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Tremeau O.etal.: "Genetic engineering of snake toxins" J.Biol.Chem.270. 9362-9369 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Segalas I.etal.: "Solution structure of green mamba toxin that activate muscarinic acetylcholine recepters, as studied by nuclear magnetic resonance and molecular modeling." Biochemistry. 34. 1248-1260 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Harvey A.L.et al: "Structure-activity studies on scorpion toxins that block potassium channels" Toxicon. 33. 425-436 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Maillere B.et al: "Immunogenicity of a disulphide-containing neurotoxin : presentation to T-cells requires a reduction step" Toxicon. 33. 475-482 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Pauplais M.et al: "An NMR study of the interaction of cardiotoxin gamma from Najanigricollis with perdeuterated dodecylphosphocholine micelles" Eur.J.Biochem.230. 213-220 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Vita C.etal.: "Scorpion toxins as natrural scaffolds for protein engineering" Proc.Natl.Acad.Sci.U.S.A.92. 6404-6408 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] S.Ohno,N.Tamiya,H.Cogger,K.Zimmerman,M.Toriba and T.Tamiya.: "Gene structures of short chain neurotoxins from genus Laticauda in South Pacific area" (発表予定).

    • Related Report
      1994 Annual Research Report
  • [Publications] H.Hatanaka,M.Oka,D.Kohda,S.Tate,A.Suda,N.Tamiya and F.Inagaki: "Tertiary structure of erabutoxin b in aqueous solution as elucidated by two dimensional nuclear magnetic resonance." J.Mol.Biol.240. 155-166 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] D.Jerusalinsky and A.L.Harvey: "Toxins from mamba venoms:Small proteins with selectivites for different subtypes of muscarinic acetylcholine receptors" Trends Pharmacol.Sci.15. 424-430 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] S.Zinn-Justin,L.Pillet,F.Ducancel,A.Thomas,J.C.Smith,J-C.Budain,A.Menez: "Engineering of Protein epitopes:A single deletion in a snake toxin generates full binding capacity to a previously unrecognized antibody" Protein Eng.7. 917-923 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] A.Bilwes,B.Rees,I,Moras,R.Menez,and A.Menez: "X-ray structure at 1.55 Å of toxin gamma,a cardio toxin from Naja nigricollis venom.Crystal packing reveals a model for insertion into membranes." J.Mol.Biol.239. 122-136 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] A.Barfaraz,A.L.Harvey: "Screening of Snake venoms for neurotoxic and myotoxic effects using simple in vitro preparations from rodents and chicks" Toxicon. 32. 267-272 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Harold Cogger: "Reptiles & Amphibians of Australia" Reed International Books, 788 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] T.Tamiya,Y.Tabara,T.Tsuchiya: "Cloning and sequence analysis of cDNA encoding phospholipase A_2 precursor from sea snake Laticauda semifasciata" Toxicon. (予定).

    • Related Report
      1993 Annual Research Report
  • [Publications] T.Tamiya,T.Ohno,E.Nishimura,T.Tsuchiya: "Cloning and sequence analysis of cDNA encoding long chain neuro toxin precursor from sea snake Laticauda semifasciata" Toxicon. (予定).

    • Related Report
      1993 Annual Research Report
  • [Publications] K.Zimmerman,M.Toriba,H.Cogger,N.Tamiya,T.Tamiya: "Reptiles in Niuafo'ou,Tonga" (予定).

    • Related Report
      1993 Annual Research Report
  • [Publications] E.Lajeunesse,F.Ducancel,D.Gillct,O.Tremeau,P.Drevet,J-C.Boulain,T.Tamiya,A.Menez: "Molecular Biology of Sea-Snake Toxins" Venom & Toxin Research Group,National University of Singapore(予定),

    • Related Report
      1993 Annual Research Report

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Published: 1993-04-01   Modified: 2016-04-21  

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