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Studies on New World leishmaniasis and its transmission

Research Project

Project/Area Number 05041115
Research Category

Grant-in-Aid for international Scientific Research

Allocation TypeSingle-year Grants
SectionField Research
Research InstitutionKochi Medical School

Principal Investigator

HASHIGUCHI Yoshihisa  Kochi Medical School, Faculty of Medicine, Professor, 医学部, 教授 (10037385)

Co-Investigator(Kenkyū-buntansha) フラード M.H.  グアヤキル大学, 医学部, 教授
ラッソー S. R.F.  グアヤキル大学, 医学部, 教授
ルンベア G. J.  グアヤキル大学, 医学部, 教授
ゴメス E.A.  カトリカ大学, 医学部, 教授
MATSUMOTO Yoshitsugu  University of Tokyo, Institute of Animal Resource Sciences, Associate Professor, 農学部, 助教授 (00173922)
KATAKURA Ken  The Jikei University School of Medicine, Faculty of Medicine, Assistance Profess, 医学部, 講師 (10130155)
MIMORI Tatsuyuki  Kumamoto University School of Medicine, Faculty of Medicine, Associate Professor, 医学部, 助教授 (00117384)
ESHITA Yuki  Kurume University School of Medicine, Faculty of Medicine, Assistance Professor, 医学部, 講師 (10082223)
HOSOKAWA Atsushi  University of the Ryukyus, Faculty of Medicine, Assistance Professor, 医学部, 講師 (10181497)
FURUYA Masato  Kochi Medical School, Faculty of Medicine, Associate Professor, 医学部, 助教授 (00035437)
NONAKA Shigeo  University of the Ryukyus, Faculty of Medicine, Professor, 医学部, 教授 (10039571)
LAZO S. Ramon f  Universited de Guayaquil.Facultad de Ciencias Medicas, Professor
GUZMAN Jose rumbea  Universitad de Guayaquil Facultad de Ciencias Medicas, Professor
GOMEZ L. Eduardo a  Universitad de Catolica Sentiago de Guayaquil, Facultad de Medicina, Professor
JURADO S. Miguel h  Universited de Guayaquil, Facultad de Ciencias Medicas, Professor
ルンベアG. J  グアヤキル大学, 医学部, 教授
森 順彦  日本歯科大学, 歯学部, 助手 (20256986)
J.ルンベアG.  グアヤキル大学, 医学部, 教授
Project Period (FY) 1993 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥20,000,000 (Direct Cost: ¥20,000,000)
Fiscal Year 1995: ¥7,000,000 (Direct Cost: ¥7,000,000)
Fiscal Year 1994: ¥6,000,000 (Direct Cost: ¥6,000,000)
Fiscal Year 1993: ¥7,000,000 (Direct Cost: ¥7,000,000)
KeywordsEcuador / Cutaneous leishmaniasis / Patients / Mephaquin / Plasmotrim / Experimental animals / Oral treatment / Skin biopsy / Antimalarial drugs / 抗マラリア剤 / メフロキン / アルテサネート / 電顕病理 / アネルギー / エクアドル / サシチョウバエ / 二次感染 / Leishmania / 皮膚病変 / 分子生物学
Research Abstract

In this fiscal year, the following items were mainly carried out : 1) the treatment of cutaneous leishmaniasis patients with antimalarial drugs ; 2) identification of parasites isolated in different endemic areas of Ecuador ; and 3) some laboratory experiments using materials collected in the field.
1) When 115 isolates from Ecuador were examined by ELISA using 9 monoclonal antibodies, 66 were L.panamensis ; 18, L.mexicana ; 8, L.major-like ; 4, L.braziliensis ; and 2. L.equatorensis. however, 17 isolates were not identified by the present method.
2) When Ecuadorian patients with cutaneous leishmaniasis were treated with Mephaquin and Plasmotrim, antimalarial drugs, the majority of them were curative between 2 and 4 weeks after the commencement of the oral treatment showing 100% cure rate. The results obtained in this study are excellent when compared with the parenteral treatment with antimonials. Therefore, the effects of these antimalarial drugs should be tested for other clinical forms of leishmaniasis such as DCL,VL,MCL and PKDL.
3) In laboratory studies using the antimalarial drugs mentioned above, no clear-cut curative effect was observed in mice or other experimental animals. Such a discrepancy between animals and humans might be caused by the difference of the action mode of the drug used.
4) Electron microscopic examinations of skin biopsies from treated patients with the antimalarial drugs revealed that there was an interesting morphological change in Leishmania organisms and the infected cells, macrophages.
5) In order to know the role of P-glycoprotein from Ecuadorian Leishmania amazonensis, the relating gene was isolated and all the base pair arrangement was sequenced. The results obtained suggested that the gene might be a new P-glycoprotein of the genus Leishmania.

Report

(3 results)
  • 1995 Final Research Report Summary
  • 1994 Annual Research Report
  • 1993 Annual Research Report
  • Research Products

    (29 results)

All Other

All Publications (29 results)

  • [Publications] 野中薫雄: "熱帯と皮膚の関わり(2)" 西日本皮膚科. 57. 29-298 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 細川 篤: "エクアドル共和国太平洋岸の低地地域に於ける皮膚リーシュマニア症に対する2%Fluorouracil(5FU)外用療法の試み" 沖縄医学会雑誌. 33. 44-47 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Eduardo A.Gomez Landires: "Oral treatment of New World cutaneous leishmaniasis with anti-malarial drugs in Ecuador: A preliminary traial" Japanese Journal of Tropical Medicine and Hygine. 23. 151-157 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 片倉 賢: "リーシュマニア症の薬物療法とリーシュマニアの薬剤耐性" メディヤサークル. 40. 285-293 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 野中薫雄: "リーシュマニア症" 医学のあゆみ. 175. 430-433 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 橋口義久: "皮膚リーシュマニア症" 皮膚科の臨床. 4. 1-10 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Hashiguchi,Y.(ed.): "Estudios sobre la Leishmaniasis en al Nuevo Mundo y su Transmision con Especial Referencia al Ecuador.No.1" Kyowa Printing Co.Ltd.Kochi,Japan, 93 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Hashiguchi,Y.(ed.): "Estudios sobre la Leishmaniasis en el Nuevo Mundo y su Transmision con Especial Referencia al Ecuador.No.2" Kyowa Printing Co.Ltd.Kochi,Japan, 148 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Nonaka Shigeo: "A relation between Tropics and skin" NISHINIHON HIFUKA. 57. 290-298 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Hosokawa Atsushi: "A trial of topical treatment with 2% Fluorouracil (5FU) against cutaneous leishmaniasis on the pacific coastal Jowland of Ecuador" OKINAWA MEDICAL JOURNAL. 33. 44-47 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Eduardo A.Gomez Landires: "Oral treatment of New World cutaneous leishmaniasis with anti-malarial drugs in Ecuador : A preliminary trial" Japanese Journal of Tropical Medicine and Hygine. 23. 151-157 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Katakura Ken: "Chemotherapy of leishmaniasis and drug resistance of the agent" Media Circle. 40. 285-293 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Nonaka Shigeo: "Leishmaniasis" IGAKUNO AYUMI. 175. 430-433 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Hashiguchi Yoshihisa: "Cutaneous leishmaniasis" HIFUKA NO RINSHO. 4. 1-10 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Hashiguchi, Y.(ed.): Kyowa Printing Co.Ltd.Kochi, Japan. Estudios sobre la Leishmaniasis en el Nuevo Mundo y su Transmision con Especial Referencia al Ecuador, 1-93 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Hashiguchi, Y.(ed.): Kyowa Printing Co.Ltd.Kochi, Japan. Estudios sobre la Leishmaniasis en el Nuevo Mundo y su Transmision con Especial Referencia al Ecuador, 1-148 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Bhutto,A.M.: "Comparative observations of golden hamsters infected with L.(L.)mexicana from Ecuadorian patients with diffuse and localized type of cutaneous leishmaniasis." Pakistan Journal of Dermatolog. 3. 17-32 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Bhutto,A.M.: "Histopathological and electron microscopical features of skin lesions in a patient with bartonellosis." Journal of Dermatology. 21. 178-184 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 橋口 義久: "新大陸のリーシュマニア症とその伝播機構,とくにアンデス型のUta症を中心に" 寄生虫学雑誌. 43. 173-186 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Hoshokawa A.: "Seroepidemiological surveys for leprosy in Ecuador" Japanese Journal of Tropical Medicine and Hygiene. 22. 179-184 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Hoshokawa A.: "Case teport of leprosy and a trial of screenings for the family members in Ecuador" Japanese Journal of Tropical Medicine and Hygiene. 22. 219-223 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Hashiguchi,Y.(ed.): "Studies on New World leishmaniasis and its transmission,with particular reference to Ecuador." Kyowa Printing Co.Ltd.Kochi.Japan, 193 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Katakura,K.: "Molecular karyotype characterization of Leishmania panamensis,Leishmania mexicana,and Leishmania major-like parasites." American,Journal of Tropical Medicine and Hygiene. 48. 707-715 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Bhutto,A.M.: "Comparative observations of golden hamsters infected with L.(L.)mexicana from Ecuadorian patients with diffuse and localized type of cutaneous leishmaniasis." Pakistan Journal of Dermatology. (in press). (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] Bhutto,A.M.: "Histopathological and electron microscopical features of skin lesions in a patient with bartonellosis." Journal of Dermatology. (in press). (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] Alava,J.J.P.: "Leishmaniasis in an endemic focus on the Pacific coast of Ecuador." Bulletin of Pan American Health Organization.(in press). (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] 野中薫雄: "エクアドルにおけるリーシュマニア症の皮膚科学的,寄生虫学的研究" 西日本皮膚科. (印刷中). (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] Hashiguchi,Y.: "Andean leishmaniasis in Ecuador." Japanese Journal of Parasitology. (投稿中). (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] Hashiguchi,Y.(ed.): "Studies on New World leishmaniasis and its transmission,with particular reference to Ecuador." Kyowa Printing Co.Ltd.Kochi,Japan, 190 (1994)

    • Related Report
      1993 Annual Research Report

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Published: 1993-04-01   Modified: 2016-04-21  

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