| Project/Area Number |
05042004
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| Research Category |
Grant-in-Aid for international Scientific Research
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| Allocation Type | Single-year Grants |
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| Section | Special Cancer Research |
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| Research Institution | Research Institute for Nuclear Medicine & Biology, Hiroshima University |
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Principal Investigator |
KAMADA Nanao Research Institute for Nuclear Medicine & Biology, Hiroshima University, 原爆放射能医学研究所, 教授 (00034629)
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| Co-Investigator(Kenkyū-buntansha) |
LI Kenpa Institute of Hematology, Chinese Academy of Medical Sciences, 血液学研究所, 研究員
KATSU Shusei Nantong Medical College, 講師
TANAKA Kimio Research Institute for Nuclear Medicine & Biology, Hiroshima University, 原爆放射能医学研究所, 助手 (70116622)
UEDA Ryuzo Research Institute, Aichi Cancer Center, 研究所, 部長 (20142169)
KIKUCHI Masahiro School of Medicine, Fukuoka Univesity, 医学部, 教授 (80078774)
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| Project Period (FY) |
1993
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| Project Status |
Completed (Fiscal Year 1993)
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| Budget Amount *help |
¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 1993: ¥3,200,000 (Direct Cost: ¥3,200,000)
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| Keywords | Non-Hodgkin lymphoma / Chronic lymphocytic leukemia / Chromosome analysis / Molecular genetic analysis / PCR / India / FISH |
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| Research Abstract |
The incidence of non-Hodgkin's lymphoma (NHL) in Japan is one-half of that in the USA. The present study was under taken to understand the incidence of follicular lymphoma and chronic lymphocyte leukemia in Japan and India in comparison to the data from the western countries, and also to investigate the differences among these countries in cytogenetic and molecular genetic findings.
1)Cytogenetic analysis of 73 Japanese NHL cases revealed a lower incidence of 14 ; 18 translocation among follicular lymphoma (40%), compared with the incidence in American cases (75-88%). Japanese CLL cases had almost the same incidence of karyotype abnormalities (45.8%, 51.5%, in USA) and types of abnormalities as American cases (trisomy 12, 14q and 13q abnormalities).
However, a higher incidence of complex karyotype was found in Japanese cases than that of western countries (p<0.05).
2)The incidence of BCL-2 rearrangement in Japanese/Indian follicular lymphoma was less than that of American cases (45.2% vs 90% USA cases in reports) by Southern blotting, 10.9% vs 53.4% USA cases by PCR. The incidence of BCL-2 rearrangement was not different between that of Japanese and American CLL cases (5.7% vs 6.8-9.3%).
3)Detection of trisomy 12 and RB gene loss by means of FISH method in CLL cases revealed that American and Indian cases had a higher incidence of trisomy 12 than that of Japanese cases (30-42.9% vs 19%), whereas RB gene loss of Indian cases (39%) was not significantly different from Japanese cases (28.6%). In general, Indian cases showed almost the same biological characteristics of Japanese B-cell malignancies, although there exists some differences with the American and European cases.
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