• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

The Characteristics of Thermo-Stable RecA Protein : The Molecular Mechanisms in Genetic Recombination

Research Project

Project/Area Number 05044132
Research Category

Grant-in-Aid for international Scientific Research

Allocation TypeSingle-year Grants
SectionJoint Research
Research InstitutionOsaka University

Principal Investigator

OGAWA Hideyuki  Faculty of Science, Osaka University, 理学部, 教授 (70028207)

Co-Investigator(Kenkyū-buntansha) YURI V.Kil  ペテルスブルグ 核物理学研究所, 分子放射線生物学部門・分子遺伝学研究室, 研究員
ALEXSEEV Andrej a.  St.Petersburg Nuclear Physics Institute in Russia, 分子遺伝学部門, 研究員
LANZOV Vladi  ペテルスブルグ 核物理学研究所, 分子遺伝学部門, 部長
KURAMITSU Seiki  Faculty of Science, 理学部, 教授 (60153368)
ITOH Tateo  Faculty of Science, 理学部, 助教授 (40051817)
OGAWA Tomoko  Faculty of Science, 理学部, 講師 (80028208)
LANZOV Vladislav a.  St.Petersburg Nuclear Physics Institute in Russia
KIL Yuri v.  St.Petersburg Nuclear Physics Institute in Russia
MINICASIMOVI イバチュリン ファ  ペテルスブルグ核物理学研究所, 分子放射線生物学部門, 研究員
KONSTANTINOV カボエフ オレ  ペテルスブルグ核物理学研究所, 分子放射線生物学部門, 室長
NIKITCHNA Zl  ペテルスブルグ物性物理学研究所, 室長
ALEXSEEVICH アレキセエフ アント  ペテルスブルグ核物理学研究所, 分子遺伝学, 研究員
ALEXSANDROVI ランゾフ ウ  ペテルスブルグ核物理学研究所, 分子遺伝学, 部長
Project Period (FY) 1993 – 1994
Project Status Completed (Fiscal Year 1994)
Budget Amount *help
¥5,000,000 (Direct Cost: ¥5,000,000)
Fiscal Year 1994: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1993: ¥3,500,000 (Direct Cost: ¥3,500,000)
Keywordsthe recA gene of thermophilic bacteria / RecA2278-5 / St.Petersburg Nuclear Physics Institute in Russia / temperature sensitive RecA protein / 温度感受性recA2278-5変異 / RecAのα-ヘリックスHの変異 / 好熱性菌のコドン使用頻度 / ATP結合蛋白質の共通アミノ酸配列 / RecA蛋白質 / 好熱菌recA遺伝子のクローニング / 熱安定性RecA蛋白質 / 組換え機構の普遍性 / RecA蛋白質の構造と機能の関連
Research Abstract

Among important problems in recombination, one of the hottest problems is to reveal a relationship between structure and function of RecA protein.To pursue this problem, it is inevitable to have suitable mutants in the recA gene.We learned that having many interesting recA mutants in phenotype, Dr.Lanzov, St.Petersburg Nuclear Physics Institute in Russia, was not familiar in physico-chemical analysis of RecA protein.Therefore we agreed to do a joint work using his useful recA mutants.Furthermore, he also has a big collection of various kinds of thermophilic bacteria, proteins of which are stable enough for sever conditions during physico-chemical analysis.We also agreed to isolate a suitable recA gene from a thermophilic bacteria to provide a heat stable RecA protein to facilitate physico-chemical analyzes.Dr.Lanzov with his colleagues came to our lab and did the joint works for these two years and obtained following results.
RecA2278-5 is a mutant RecA protein bearing two amino acid substitutions, Gly278 by Thr and Val275 by Phe, in the a-helix H of C-terminal sub-domain of the RecA.The mutant is defective in genetic recombination and SOS-repair at 42*C.We compared the biochemical activities of RecA2278-5 with those of a wild type RecA protein at 32゚and 42゚C.The thermosensitive multiple deficiencies of the RecA2278-5 protein suggest that the structural stability of C-subdomain of the RecA protein is necessary for RecA-ATP-ssDNA helix filament formation that is a primary step of homologous recombination in bacteria.
The recA gene of a thermophilic bacteria was successfully isolated by PCR methods, in which the primers were designed after a common amino acid sequence for an ATP binding domain of RecA proteins.Nucleotide sequence analysis revealed that most of the third nucleotide in a codon is G or C.Therefore the DNA is GC rich and heat-stable. This characteristic is quite reasonable for thermophilic bacteria.

Report

(2 results)
  • 1994 Final Research Report Summary
  • 1993 Annual Research Report
  • Research Products

    (58 results)

All Other

All Publications (58 results)

  • [Publications] Ryuichi Kato and Hideyuki Ogawa: "An essential gene,ESR1,is required for mitotic cell growth,DNA repair and meiotic recombination in Saccharomyces cerevisiae" Nucleic Acids Research. 22. 3104-3112 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Katsyki Johzuka and Hideyiki Ogawa: "Interaction of Mre11 and Rad5-:Two protein required for DNA repair and meiosis-specific double-strand break formation in Saccharomyces cerevisiae" Genetics. 139(in press). (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Hideyuki Ogawa,Katsuki Johzuka,Takuro Nakagawa,Sunhee,Leem and A.H.Hagiwara: "Functions of the yeast meiotic recombination genes,MRE11 and MRE2" Adv.Biophysics. 31. 67-76 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Hitoshi Kurumizawa,Shukuko Ikawa,Tomoatsu Ikeya,Tomoko Ogawa and Takehiko Shibata: "A chimera RecA protein exhibits altered double-stranded DNA-binding." J.Biol.Chem.269. 3068-3075 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Tomoko Ogawa,Akira Shinohara,Akira Nabetani,Tomoatsu Ikeya,Xiong Yu,Edward H.Egelman and Hideyuki Ogawa: "RecA-like Recombination Proteins in Eukaryotes:Functions and Structures of RAD51 Genes." Cold Spring Harbor Symp.Quant.Biol.58. 567-576 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Hitoshi Kurumizaka,B.J.Rao,Tomoko Ogawa,Charles M.Radding and Takehiko Shibata,: "A Chimeric RecA protein that implicates non-Watson-Crick interaction in homologous pairing" Nucleic Acid Res.22. 3387-3391 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Tomoko Ogawa Akira Shinohara and Tomoatsu Ikeya: "A species-specific interaction of Rad51 and Rad52 proteins" Adv.Biophysics.31. 93-100 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Masahiro Terasawa,Akira Shinohara,Yasuo Hotta,Hideyuki Ogawa,and Tomoko Ogawa: "Localization of RecA-like Recombination Proteins on Chromosomes of Lily at Various Meiotic Stages." Genes & Development. (in press). (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kil'Yu.V.,Goryshin I.Yu.,Lantsov V.A: "Recombinational mechanism of IS50 mobile element precise excision in Escherichia coli K12 cells" Molecular Biology. 23. 373-379 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Lantsov V.A.: "Ideal gene therapy:approaches and horizons" Molecular Biology. 28. 321-327 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Namsaraev E.A.,Akhmedov A.T.,Lantsov V.A.: "Two types of DNA-pairing enzymic activities in rat spermatocyte nuclei partial purification and characterization" Molecular Biology. 28. 499-502 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Benjukh D.N.,Silbergleit Arc.S.,Chepurin I.V.,Ponomarenko M.P.,Kolchanov N.A.and V.A.Lanzov.: "Structure-function relationship in bacterial RecA protein:computer analysis of the primary structure of RecA protein family" N.A.Kolchanov and H.A.Lim(eds.),In Computer analysis of genetic macromolecules:structure,function and evolution.World Scientific Pub.Co.,Singapore. 469-477 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Zaitsev E.,Alexseyev A.,Lanzov V.,Satin L.,Clark J.A.: "Nucleotide sequence between reca and alaSp in E.coli K12 and the sequence change in four recA mutations" Mutation Research.323. 173-177 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kil Yu.V.,Goryshin I.Yu.,Lanzov V.A: "Transmids:particularities of transposition,application for direct DNA sequencing of conjugative plasmid" Dokl.Acad.Sci.Russia. 335. 251-254 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kil Yu.V.,Goryshin I.Yu.,Lanzov V.A.: "Nature of integration specificity for IS50 mobile element in Escherichia coli" Doki.Acad.Sci.Russia.339. 705-709 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Matsushima,Y.,Kim,D.-W.,Yoshimura,T.,Kagamiyama,H.Esaki,N.,and Kuramitsu,S.: "Replacement of Active Site Lysine-239 of Thermost-able Aspartate Amino-transferase by S-(2-Aminoethyl)Cysteine:Properties of the Mutant Enzyme" J.Biochem.115. 108-112 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Miyazawa,K.,Kawaguchi,S.,Okamoto,A.,Kato,R.,Ogawa,T.,and Kuramitsu,S: "Construction of Aminotransferase Chimeras and Analysis of Their Substrate □ Specificity" J.Biochem. 115. 568-577 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Okamoto,A.,Higuchi,T.,Hirotsu,K.,Kuramitsu,S.,and Kagamiyama,H.: "X-Ray Crystallographic Study of Pyridoxal 5'-Phosphate-Type Aspartate Aminotransferase from Escherichia coli in Open and Closed Forms" J.Biochem. 116. 95-107 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Watanabe,R.,Masui,R.,Kato,R.,and Kuramitsu,S: "Interaction of Escherichia coli RecA Protein with ATP and Its Analogues" J.Biochem. 116. 960-966 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kawaguchi,S.and Kuramitsu,S.□: "Homologus Ligation" Trends Genet.10. 420 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Metzler,D.E.,Metzier,C.M.,Scott,R.D.,Mollova,E.T.,Kagamiyama,H.,Yano,T.,Kuramitsu,S.,Hayashi,H.,Hirotsu,K.,and Miyahara,I.: "NMR Studies of 1H Resonances in the 10-18-ppm Range for Aspartate Aminotransferase from Escherichia coli" □ J.Biol.Chem.269. 28027-28033 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kawaguchi,S.and Kuramitsu,S.□: "Separation of Heat-Stable Proteins from Thermus thermophilus HB8 by Two-Dimensional Electrophoresis" Electophoresis. (in press). (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Mikawa,T.,Masui,R.,Ogawa,T.,Ogawa,H.,and Kuramitsu,S.(1995) □: "N-Terminal 33 Amino Acid Residues of Escherichia coli RecA Protein Contribute to Its Self-Assembly" J.Mol.Biol.(in press). (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 小川智子 篠原 彰 小川英行: "真核生物の組換え蛋白質Rad51とRad52の機能と構造" 実験医学(羊土社)「トッピクス」. 12. 524-547 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 小川英行、小川智子: "酵母に観る最新の真核生物像 VI.組換え機構 減数分裂期組換え-関与する遺伝子とその普遍性-" 蛋白質 核酸 酵素(共立出版)「臨時増刊号」. 39. 568-578 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 篠原 彰 小川英行 小川智子: "真核生物の組換え(修復)に働く遺伝子" 細胞工学 (秀潤社). 13. 710-719 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 小川智子: "真核生物の組換え蛋白質の機能と構造" 第25回 放射性総合医学研究所シンポジウム. 25. 25-37 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 篠原 彰 小川英行 小川智子: "真核生物RAD51遺伝子の構造と機能" 日本臨床 (日本臨床社). 53. 239-249 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 河口真一,倉光成紀: "酵素反応のダイナミックス" 蛋白質 核酸 酵素. 39. 1083-1090 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Ryuichi Kato and Hideyuki Ogawa: "Nucleic Acid Res." An essential gene, ESR1, is required for mitotic cell growth, DNA repair and meiotic recombination in Saccharomyces cerevisiae. 22. 3104-3112 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Katsyki Johzuka and Hideyuki Ogawa: "Interaction of Mre11 and Rad5- : Two protein required for DNA repair and meiosis-specific double-strand break formation in Saccharomyces cerevisiae" Genetics. 139. Inpress (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Hideyuki Ogawa, Katsuki Johzuka, Takuro Nakagawa, Sun hee, Leem and A.H.Hagiwara: "Functions of the yeast meiotic recombination genes, MRE11 and MRE2" Adv.Biophysics. 31. 67-76 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Hitoshi Kurumizaka, Shukuko Ikawa, Tomoatsu Ikeya, Tomoko Ogawa and Takehiko Shibata: "A chimera RecA protein exhibits altered double-stranded DNA-binding" J.Biol.Chem. 269. 3068-3079 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Tomoko Ogawa, Akira Shinohara, Akira Nabetani, Tomoatsu Ikeya, Xiong Yu, Edward H.Egelman and Hideyuki Ogawa: "RecA-like Recombination Proteins in Eukaryotes : Functions and Structures of RAD51 Genes" Cold Spring Harbor Symp.Quant.Biol. 58. 567-576 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Hitoshi Kurumizaki, B.J.Rao, Tomoko Ogawa, Charles M.Radding and Takehiko Shibata: "A chimeric RecA protein that implicates non-Watson-Crick interaction in homologous pairing" Nucleic Acid Res.22. 3387-3391 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Tomoko Ogawa Akira Shinohara and Tomoatsu Ikeya: "A species-specific interaction of Rad51 and Rad52 proteins" Adv.Biophysics. 31. 93-100 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Masahiro Terasawa, Akira Shinohara, Yasuo Hotta, Hideyuki Ogawa and Tomoko Ogawa.: "Localization of RecA-like Recombination proteins on Chromosomes of Lily at Vanious Meiotic Stages" Genes & Development. inpress (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kil'Yu.V., Goryshin I.Yu., Lantsov V.A: "Recombinational mechanism of IS50 mobile element precise excision in Escherichia coli K12 cells" Molecular Biology. 28. 373-379 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Lantsov V.A: "Ideal gene therapy : approaches and horizons" Molecular Biology. 28. 321-327 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Namsaraev E.A., Akhmedov A.T., Lantsov V.A: "Two types of DNA-pairing enzymic activities in rat spermatocyte nuclei : partial punification and characterization" Molecular Biology. 28. 499-502 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Benjukh D.N., Silbergleit Arc.S., Chepurin I.V., Ponomarenko M.P., Kolchanov N.A.and V.A.Lanzov: "Structure-function relationship in bacterial RecA protein : computer analysis of the primary structure of RecA protein family" N.A.Kolchanov and H.A.Lim(eds.), Computer analysis of genetic macromolecules : structure, function and evolution.World Scientific Pub.Co., Singapore. 469-477 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Zaitsev E., Alexseyev A., Lanzov V., Satin L., Clark J.A: "Nucleotide sequence between reca and alaSp in E.coli K12 and the sequence change in four recA mutations" Mutation Research.1994.323. 173-177 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kil Yu.V., Goryshin I.Yu., Lanzov V.A.: "Transmids : particularities of transposition, application for direct DNA sequencing of conjugative plasmid" Dokl.Acad.Sci.Russia.1994.335(2) : 251-254.335. 251-254 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kil Yu.V., Goryshin I.Yu., Lanzov V.A.: "Nature of integration specificity for IS50 mobile element in Escherichia coli" Dokl.Acad.Sci.Russia.1994.339(5) : 705-709. 339. 705-709 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Matsushima, Y., Kim, D.-W., Yoshimura, T., Kuramitsu, S., Kagamiyama, H., and Esaki, N.: "Replacement of Active Site Lysine-239 of Thermostable Aspartate Aminotransferase by S-(2-Aminoethyl)Cysteine : Properties of the Mutant Enzyme" J.Biochem.115. 108-112 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Miyazawa, K., Kawaguchi, S., Okamoto, A., Kato, R., Ogawa, T., and Kuramitsu, S.: "Construction of Aminotransferase Chimeras and Analysis of Their Substrate Specificity" J.Biochem.115. 568-577 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Watanabe, R., Masui, R., Kato, R., and Kuramitsu, S: "Interaction of Escherichia coli RecA Protein with ATP and Its Analogues" J.Biochem.116. 960-966 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Okamoto, A., Higuchi, T., Hirotsu, K., Kuramitsu, S., and Kagamiyama, H.: "X-Ray Crystallographic Study of Pyridoxal 5'-Phosphate-Type Aspartate Amino transferase from Escherichia coli in Open and Closed Forms" J.Biochem.116. 95-107 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kawaguchi, S.and Kuramitsu, S.: "Homologus Ligation" Trends Genet.10. 420 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Metzler, D.E., Metzler, C.M., Scott, R.D., Mollova, E.T., Kagamiyama, H., Yano, T., Kuramitsu.S., Hayashi, H., Hirotsu, K., and Miyahara, I.: "NMR Studies of 1H Resonances in the 10-18-ppm Range for Aspartate Amino transferase from Escherichia coli" J,Biol.Chem.269. 28027-28033 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kawaguchi, S.and Kuramitsu.S: "Separation of Heat-Stable Proteins from Thermus thermophilus HB8 by Two-Dimensional Electrophoresis" Electophoresis. inpress (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Tsutomu Mikawa, Ryoji Masui, Tomoko Ogawa, Hideyuki Ogawa and Seiki Kuramitsu: "N-terminal 33 amino acid residues of Escherichia coli RecA protein contribute to its self-assembly" J.Mol.Biol.Submitted.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] T.Ogawa,A.Shinohara,A.Nabetani,T.Ikeya,X.Yu,E.H.Egelman and H.Ogawa: "RecA-like Recombination Proteins in Eukarvotes:Functions and structures of RAD 51 Genes" Cold Spring HarborSymp.Quant.Biol. in press. 58.

    • Related Report
      1993 Annual Research Report
  • [Publications] H.Kurumizaka,S.Ikawa,T.Ikeya,T.Ogawa and T.Shibata: "A Chimeric RecA protein exhibits altered double-stranded DNA binding" J.Biol.Chem.269. 3068-3075 (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] O.Bezzubova,A.Shinohara,R.G.Muueller,H.Ogawa and J-M.Buerstedde: "A chicken RAD51 homologue is expressed at high levels in lymphoid and reproductive organs" Nucleic Acids Research. 21. 1577-1580 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] A.Shinohara,H.Ogawa,Y.Matsuda,N.Ushio,K.Ikeo and T.Ogawa: "Clonig of Human,Mouse and Fission Yeast Recombination GeneT7 Homologous to RAD51 and recA" Nature Genetics. 4. 239-243 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] T.Ogawa,X.Yu,A.Shinohara and E.H.Egelman: "Similarity of the Yeast Rad 51 Filament to the Bacterial RecA Filament" Science. 259. 1896-1899 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] M.Ajimura,S-H.Leem and H.Ogawa: "Identification of new genes required for meiotic recombination in Saccharomyses cerevisiae" Genetics. 133. 51-66 (1993)

    • Related Report
      1993 Annual Research Report

URL: 

Published: 1993-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi