• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Regulation of Gene Expression

Research Project

Project/Area Number 05044150
Research Category

Grant-in-Aid for international Scientific Research

Allocation TypeSingle-year Grants
SectionJoint Research
Research InstitutionChiba University

Principal Investigator

TANIGUCHI Masaru  Chiba University, School of Medicine Professor, 医学部, 教授 (80110310)

Co-Investigator(Kenkyū-buntansha) KYO Masaharu  Kagawa Univ., 農学部, 助教授 (70195395)
WATANABE Masao  Tohoku Univ., 農学部, 助手 (90240522)
OKADA Kiyotaka  Tokyo Univ., 理学部, 教授 (50101093)
KOMEDA Yoshifumi  Hokkaido Univ., 理学部, 教授 (10124215)
中世古 幸信  京都大学, 理学部, 助手 (30231468)
KATO Koichi  Tokyo Univ., 薬学部, 助手 (20211849)
MURAKAMI Yuji  Japan Advanced Institute of Science and Technology Hokuriku, 助手 (70272995)
YOSHIDA Minoru  Tokyo Univ., 農学部, 助手 (80191617)
ISOGAI Akira  Japan Advenced Institute of Science and Technology Nara, 教授 (20011992)
KAMADA Hiroshi  Tsukuba Univ., 生物科学系, 教授 (00169608)
WAKASUGI Hiro  National Insti. Cancer Center, がん宿主免疫研, 室長 (10112632)
SASAZUKI Takehiko  Kyushu Univ., 生体防御医学研究所, 教授 (50014121)
KISHIMOTO Tadamitsu  Osaka Univ., 医学部, 教授 (10093402)
OKUMURA Ko  Juntendo Univ., 医学部, 教授 (50009700)
SHINOZAKI Kazuo  The Institute of Physical and Chemical Research, ライフサイエンス筑波研究センター, 研究員 (20124216)
FUJII Tadashi  Tsukuba Univ., 生物科学系, 教授 (20011611)
YAMADA Tetsuji  Okayama Univ., 農学部, 教授 (00191320)
DOKE Noriyuki  Nagoya Univ., 農学部, 教授 (80023472)
TANAKA Keiji  Tokushima Univ., 酵素科学研究センター, 助教授 (10108871)
SAKAI Shingo  Tsukuba Univ., 生物科学系, 助教授 (60033388)
マリセン B.  CNRS, ラサンテ医研・マルセイユルミニー免疫センター, センター長
バーリング R.  GSF, 遺伝研, 教授
マティス D.  CNRS, 免疫学研究所, 室長
ベノワ C.  CNRS, 免疫学研究所, 室長
クーパー J.A.  フレッドハッチンソンがん研, 研究員
ハーガン I.M.  マンチェスター大, がん研, 研究員
ラジャウスキー K.  ケルン大, 科学, 教授
シラー F.W.  ポツダム大, バイオ化学研, 教授
URANO Naoto  Tokyo Univ. of Fisheries, 食品生産学科, 助教授 (90262336)
SHIMADA Kazuo  Tokyo Univ., 薬学部, 教授 (70196476)
HORINOUCHI Sueharu  Tokyo Univ., 農業生命科学研究所, 教授 (80143410)
SHIMURA Yoshiro  Kyoto Univ. Faculty of Science professor, 理学部, 教授 (60025426)
MATSUBARA Ken-ichi  Osaka Univ. Institute of Molecular and Cellular Siology, Professor, 細胞生体工学センター, 教授 (20037394)
YAMADA Yasuyuki  Japan Advanced Institute of Science and Technology Nara, 農学部, 教授 (50026415)
KARUBE Isao  Tokyo Univ. Research Center for Advanced Science and Technology, Professor, 先端科学技術研究センター, 教授 (50089827)
BEPPU Teruhiko  Nippon Univ. Faculty of Agriculture and Veterinary Medicin Professor, 獣医学部, 教授 (80011873)
KIKUTANI Hitoshi  Osaka Univ., 細胞生体工学センター, 助教授 (80161412)
KOSEKI Haruhiko  Chiba Univ. School of Medicine Assistant, 医学部, 助手 (40225446)
SAITO Takashi  Chiba Univ., 医学部, 教授 (50205655)
WATANABE Takeshi  Kyushu Univ. Medical Institute of Bioregulation, Professo, 生体防御医学研究所, 教授 (40028684)
デュマ C.  リヨン高等専門学校, 教授
ツジイ F.I.  カリフォルニア大, スクリプト研, 教授
ロバーツ G.C.K.  ライセスター大, 生物研, 教授
デルセニー M.  ペルピニョン大学, 教授
フォルセン K.S.  ランド大, 工, 教授
TSUJI Federick  California Univ., USA
FORSEN Karl  Lund Univ., Sweden
COOPER Jonathan  Fred Huchinson Cancer Res. Ctr, USA
HAGAN Iain  Manchester Univ., England
ROBERTS Gordon  Leicester Univ., England
NAKASEKO Nobuyuki  Kyoto Univ.
DUMAS C.  Ecole Normale Surperieure de Lyon, France
SCHELLER Frieder  Potsdam Univ.
DELSENY Michel  Univ. de Perpignan, France
MATHIS Diane  CNRS,France
BENOIST Christophe  CNRS,France
MALISSEN Bernard  CNRS,France
BALLING Rudolf  GSF,Germany
RAJEWSKY Klaus  Kologne Univ., Germany
原田 宏  筑波大学, 生物科学系, 教授 (90015991)
鈴木 正康  九州工業大学, 情報工学部, 助教授 (70226554)
和田 雅人  筑波大学, 生物科学系, 助手
長田 重一  大阪バイオサインス研究所, 第一研究部, 部長 (70114428)
菅村 和夫  東北大学, 医学部, 教授 (20117360)
西川 伸一  京都大学, 医学部, 教授 (60127115)
平野 俊夫  大阪大学, 医学部, 教授 (40136718)
木下 タロウ  大阪大学, 微生物病研究所, 教授 (10153165)
勝木 元也  九州大学, 生体防御医学研究所, 教授 (20051732)
スレイマン C.W.  エール大, 医学部, 教授
ラスキー R.  ケンブリッジ大, 生物, 教授
シュミット R.D.  シュツットガルト大, バイオプロセス工学センター, 教授
マリッセン B.  CNRS, ラサンテ医学研・マルセイユルミニー免疫センター, センター長
荒田 洋治  東京大学, 薬学部, 教授 (40011499)
中村 祐輔  (財)癌研究会, 癌研究所・生化学部, 部長 (70217909)
河原井 昌裕  西東京科学大学, バイオサイエンス科, 助教授 (60234142)
早出 広司  東京農業工業大学, 工学部, 助教授 (10187883)
野村 港二  筑波大学, 農林学系, 講師 (00183905)
安田 国雄  奈良先端科学技術大学院大学, バイオサイエンス研究科, 教授 (30025473)
大野 睦人  京都大学, 理学部, 助手 (80201979)
小林 悟  筑波大学, 生物科学系, 助手 (90225508)
菅野 雅元  千葉大学, 医学部, 助教授 (40161393)
志田 壽利  京都大学, ウイルス研究所, 助教授 (00144395)
坂本 博  神戸大学, 理学部, 助教授 (00187048)
横山 茂之  東京大学, 理学部, 教授 (00159229)
井上 丹  京都大学, 理学部, 教授 (40114855)
Project Period (FY) 1993 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥30,000,000 (Direct Cost: ¥30,000,000)
Fiscal Year 1995: ¥10,000,000 (Direct Cost: ¥10,000,000)
Fiscal Year 1994: ¥10,000,000 (Direct Cost: ¥10,000,000)
Fiscal Year 1993: ¥10,000,000 (Direct Cost: ¥10,000,000)
KeywordsFission yeast / Cre-loxP / Morphogenesis / Higher plants / Gene targeting / Protein interaction / NMR / Biosensor / 免疫系 / 免疫病 / ATPase / 温度感受性突然変異体 / 細胞周期 / DNA再複製 / バイオモニタリング / RNAプロセシング / 遺伝子ターゲティング / 蛋白高次機能構造 / 胚形成 / 植物
Research Abstract

Following results were obtained :
1. We isolated several homologues of Vp-1 or Abi 3 genes which are essential for ABA-mediated signal transduction in the regulation of higher plant morphogenesis.
2. By using gene targeting, we investigated function of several genes, including mel-18 which is a negative regulatory transcription factor and controls the expression of homeobox genes. Mel-18 knock-outs showed posterior transformation of skeletal bones, suggesting their equivalent functions to those of Drosophila.
3. By NMR,molecular interactions between IgG and protein-A,-G,and -L,were analyzed. It was revealed that 1) the IgG-binding domains of protein-A and -G have quite different three-dimensional structures, but bind to sites on the Fc that overlap extensively, 2) protein G employs two quite different regions of its surface to bind to the Fab and the Fc regions, and 3) the fold of the IgG-binding domain of protein L is similar to that of protein G,but they bind to a different domain in the Fab.
4. By indirect immunofluorescence microscopy, we analyzed location and morphological change of chromatin region and microtuble especially during mitosis in the fission yeast S.pombe cells.
5. For development of high sensitive biosensor, some devices with the chamber packing beads immobilized with microorganisms were fabricated by micromachine and CCD camera system. The system gave current response proportional to glucose concentration. Distribution of the luminescendce gave theoretical decrease as a function of low-rate and activity of immobilized enzyme. The system has ability to be highly sensitive due to chemical amplification by the enzyme induced by analyte.
6. We discussed future collaborations on France-Japan Immunology, particularly on T-B collaboration, regulation lymphocyte development, and immune diseases. Collaboration includes exchange of information, materials, and young scientists.

Report

(3 results)
  • 1995 Final Research Report Summary
  • 1994 Annual Research Report
  • 1993 Annual Research Report
  • Research Products

    (50 results)

All Other

All Publications (50 results)

  • [Publications] Kamada,H.: "Effects of light and growth regulators on adventitious bud formation in horse radish (Armoracia rustlcana)." Plant Cell Reports. 14. 611-615 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Sakamoto,K.: "A male-associated DNA sequence in a dioecious plant,Cannabis sativa L." Plant and cell Physiology. 36. 1549-1554 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Handa,T.: "Genetic transformation of Eustoma gradiflorum with rol genes." Acta Horticul turae. 392. 209-218 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Taniuchi,I.: "Antigen-receptor induced clonal expansion and deletion of lymphocytes are impaired in the mice lacking HSI protein,a substrate of antigen-receptor coupled tyrosine kinases." EMBO J.14. 3664-3678 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Fukuda,T.: "Restoration of surface IgM-mediated apoptosis in an anti-IgM resistant variant of WEHI-231 Iymphoma cells by HSI,a protein tyrosine kinase substrate." Proc.Natl.Acad.Sci.USA. 91. 7302-7306 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Nishizumi,H.: "Impaired proliferation of peripheral B cells and indication of autoimmune disease in Iyn-deficient mice." Immunity. 3. 549-560 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Ebensperger,C.: "Pax-1,a regulator of sclerotome development is induced by notochord and floor plate signals iv avian embryos." Anat.Embryology. 191. 297-310 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Akasaka,T.: "A role for mel-18,a polycomb group-releted vertebrate gene,during the anteroposterior specification of the axial skeleton." Development. In press.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Samejima,I.: "Identification of seven new cut genes involved in Schizosaccharomyces pombe mitosis." Journal of Cell Science. 105. 135-143 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Saka,Y.: "Fission yeast cut3 and cut14 members of a ubiquitous protein family,are required for chromosome condensation and segregation in mitosis." EMBO J.13. 4938-4952 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Nabeshima,K.: "p93disl.which is required for sister chromatid separation,is a novel microtubule and spindle pole body-associating protein phosphorylated at the cdc2 target sites." Genes and Development. 9. 1572-1585 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Murakami,Y.: "Micromachined enzyme reactor for FIA system." Microchemical J.49. 319-325 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kato,K.: "Model for complex between protein G and an antibody Fc fragment solution." Structure. 3. 79-85 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] G.C.K.: "Techniques in Protein Chemistry VI" Academic Press, 585 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] H.Nishizumi: "Impaired proliferation of peripheral B cells and indication of autoimmune disease in lyn-deficient mice" Immunity. 3. 549-560 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] T.Akasaka: "A role for mel-18, a polycomb group-related vertebrate gene, during the anteroposterior specification of the axial skeleton" Development. (In press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] K.Kato: "Model for the complex between protein G and an antibody Fc fragment in solution" Structure. 3. 79-85 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] I.Samejima: "Identification of seven new cut genes involved in Schizosacchoromyces pombe mitosis" Journal of Cell Science. 105. 135-143 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Y.Saka: "Fission yeast cut 3 and cut14, members of a ubiquitous protein family, are required for chromosome condensation and segregation in mitosis" EMBO J.13. 4938-4952 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] H.Kamada: "Effects of light and growth regulators on adventitious bud formation in horseradish (Armoracia rusticana)" Plant Cell Reports. 14. 611-615 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] K.Sakamoto: "A male-associated DNA sequence in a dioecious plant, Cannabis sativa L" Plant and Cell Physiology. 36. 1549-1554 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] T.Handa: "Genetic transformation of Eustoma grandiflorum with rol genes" Acta Horticulturae. 392. 209-218 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] I.Taniuchi: "Antigen-receptor induced clonal expansion and deletion of lymphocytes are impaired in the mice lacking HS1 protein, a substrate of the antigen-receptor coupled tyrosine kinases" EMBO J.14 (15). 3664-3678 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] T.Fukuda: "Restoration of surface IgM-mediated apoptosis in an anti-IgM resistant variant of WEHI-231 lymphoma cells by HS1, a protein tyrosine kinase substrate" Proc. Natl. Acad. Sci. USA. 92. 7302-7306 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] K.Nabeshima: "p93dis1, which is required for sister chromatid separation, is a novel microtubule and spindle pole body-associating protein phosphorylated at the cdc2 target sites" Genes and Development. 9. 1572-1585 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] S-M.Lee: "Bioluminescence detection system of mutagen using firely luciferase genes introduced in Escherichia celi lysogenic strain" Analytical Chemistry. 64. 1755-1759 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Y.Murakami: "Integration of enzyme immobilized column with electoro-chemical flow cell using micromachining techniques for a glucose detection system" Analytical Chemistry. 65. 2731-2735 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Adachi,Y.: "Positive selection of invariant Vα14+ T cells by non-major histocompatibility complex-encoded class-I molecules expressed on bone marrow-derived cells." Proc.Natl.Acad.Sci.USA. 92. 1200-1204 (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] Benhamou,L.E.: "Signaling properties of anti-immunoglobulin resistant variants of WEHI-231 B lymphoma cells." Eur.J.Immunology.24. 1993-1999 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Yamamoto,K.: "Functional interaction between human histocompatibility leukocyte antigen (HLA) class II and mouse CD4 molecule in antigen recognition by T cells in HLA-DR and DQ transgenic mice." J.Exp.Med.180. 165-171 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Ohno,H.: "Preferential usage of the FcRγ in the T cell antigen receptor complex by γδ T cells localized in epithelia." J.Exp.Med.179. 365-369 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Matsuda,T.: "Association of p72 tyrosine kinase with Stat factors and its activation by IL-3,IL-6 and G-CSF." Blood. 83. 3457-3461 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Kondo,M.: "Functional participation of the IL-2 receptor γ chain in IL-7 receptor complexes." Science. 263. 1453-1454 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Miyata,T.: "Abnormalities of PIG-A transcripts in granulocytes from patients with paroxysmal nocturnal hemoglobinuria." New England Journal of Medicine. 330. 249-255 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Gondo,Y.: "Gene replacement of the p53 gene with the lacZ gene in mouse embryonic stem cells and mice by using two steps of homologous recombination" Biochem.Biophys.Res.Commun.202. 830-837 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Takahashi,T.: "Gene ralized lymphoproliferative disease in mice,caused by a point mutation in the Fas ligand" Cell. 76. 969-976 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Kagi,D.: "Fas and perforin pathways as major mechanisms of T cell-mediated cytotoxicity" Science. 265. 528-530 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Usui,T.:"A K-252a-resistance gene,sks 1+,encodes a protein similar to the Caenorhabditis elegans F37A4.5,gene product and confers multidrug resistance in Schizosaccharomyces pombe." Gene. (in press). (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] Yoshirou SHIMURA: "The role of exon sequence in splice site selection" Genes & Development. 7. 407-418 (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] Shegeyuki YOKOYAMA: "Sequence specific ^1H and ^<15>N resonance assignments and secondary structure of GDP-bound human c-Ha-Ras protein in solution" Biomol.NMR. 3. 165-184 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Hiroshi SAKAMOTO: "Construction of a novel database containing aberrant splicing mutations of mammalian genes" Gene. (In press).

    • Related Report
      1993 Annual Research Report
  • [Publications] Tan INOUE: "Two universally conserved adenosine of the group I intron that are important for self-splicing but not for core catlytic activity" J.of Biochem.(In press).

    • Related Report
      1993 Annual Research Report
  • [Publications] Satoru KOBAYASHI: "Presence of mitochondrial large ribosomal RNA outside mitochondria in germ plasm of Drosophila melanogaster" Science. 206. 1521-1524 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Kunio YASUDA: "How are non-lenticular cells ready for transdifferentiation?" Develop.Dynamics. 196. 273-275 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] T.ICHIKI: "Regulation of the expressionof human C ε germline transcript:Identification of a novel IL-4 responsive element" J.Immunol.150. 5408-5417 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Koji SODE: "Subzero temperature operating biosensor utilizing an organic solvent and quinoprotein glucose dehydrogenase" Biotechnology & Bioengineering. 42. 215-254 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Masahiro KAWARAI: "Neurogenic cutaneous vasodilation in the cat forepaw" J.Autonomic Nervous System. 37. 39-46 (1992)

    • Related Report
      1993 Annual Research Report
  • [Publications] K.MASUDA: "Residual structure and constituent proteins of the peripheral framework of the cell nucleus in somatic embryos from Daucus carota L" Planta. 191. 532-540 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Atushi ISHIDA: "Cloning and chromosome mapping of DNA-binding human Mel-18 gene which possesses the new RING-finger motif" Gene. 129. 249-255 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Koichi KATO: "^<13>C NMR study of the mode of interaction in solution of the B fragment of staphylococcal protein A and the Fc fragments of mouse immunoglobulin G" FEBS Lett.328. 49-54 (1993)

    • Related Report
      1993 Annual Research Report

URL: 

Published: 1993-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi