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Studies n Functional Roles of Fatty Acid-Binding Proteins

Research Project

Project/Area Number 05044154
Research Category

Grant-in-Aid for international Scientific Research

Allocation TypeSingle-year Grants
SectionJoint Research
Research InstitutionNiigata University

Principal Investigator

ONO Teruo  Niigata Univ.Sch.of Med., Professor, 医学部, 教授 (00000927)

Co-Investigator(Kenkyū-buntansha) SAKAKIBARA Jun  Niigata Univ.Sch.of Med., Research Associate, 医学部, 助手 (90242403)
FUJII Hiroshi  Niigata Univ.Sch.of Med., Associate Professor, 医学部, 助教授 (90165340)
ISEKI Shoichi  Kanazawa Univ.Sch.of Med., Professor, 医学部, 教授 (50167251)
SUZUKI Toshimitsu  Fukushima Medical College, Professor, 教授 (80018952)
KONDO Hisatake  Tohoku Univ.Sch.of Med., Professor, 医学部, 教授 (20004723)
STORCH Judith  Rutgers University, Associate Professor, 栄養学部, 助教授
JUDITH Storc  Rutgers大学, 栄養学部, 助教授
Project Period (FY) 1993 – 1994
Project Status Completed (Fiscal Year 1994)
Budget Amount *help
¥6,500,000 (Direct Cost: ¥6,500,000)
Fiscal Year 1994: ¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 1993: ¥3,500,000 (Direct Cost: ¥3,500,000)
KeywordsFatty Acid-Binding Protein (FABP) / H-FABP / C-FABP / I-FABP / I-15P / Diabetes Mellitus / Ischaemic Intestinal Diseases / Fluorescent Fatty Acid / L-FABP / 心筋梗塞 / 脂肪酸 / ステロイド産生臓器
Research Abstract

We have studied on functional roles of cellular fatty acid-binding proteins (FABPs) in the following aspects.
(1) Transfer mechanism of FABP bound-fatty acids to membranes :
Using an in vitro fluorescence energy transfer assay, we presented the evidence that H-FABP and L-FABP bound-fatty acids are transferred to membranes through different mechanism. Fatty acids from H-FABP was transferred by collisional interaction, while the rate limiting step from L-FABP was the dissociation rate of fatty acids to aqueous medium.
(2) A new FABP (C-FABP) from rat skin :
We have purified a new subtype of H-FABP from rat skin and cloned this protein. The deduced amino acid sequence of the cDNA clone comprises residues yielding a molecular mass for the polypeptide of 15 kDa and the primary structure is homologus to H-FABP family proteins. Interestingly, it has six cysteines and two intramolecular disulfide bridges are present. This is the first presentation of disulfide formed protein among FABPs superfamily. Three dimensional structure of this new type of FABP may afford a new sight of functional roles of this protein family in the future.
(3) Regulatory mechanism of H-FABP expression in aorta :
We have analyzed the H-FABP expression in diabetic rat aorta and provided the evidence that the H-FABP gene in aorta is specifically and uniquely regulated by insulin when compared to the H-FABP expression in heart tissue. Insulin regulated H-FABP expression in aorta may be involved in diabetic complications of blood vessels.
(4) Clinical application of I-FABP :
Taking the advantage of specific expression of I-FABP limited in intestinal tissue, we have applied the I-FABP as a diagnostic tool. We have showed that the I-FABP is released into the circulation in the acute phase of intestinal ischaemia. Accordingly, the measurement of I-FABP in serum can be used in establishing the diagnosis of ischaemic intestinal diseases.

Report

(2 results)
  • 1994 Final Research Report Summary
  • 1993 Annual Research Report

Research Products

(34 results)

All Other

All Publications

  • [Publications] Watanabe,R.: "Molecular cloning of a cDNA encoding a novel fatty acid binding protein from rat skin" Biochem.Biophys.Res.Commun.200. 253-259 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Sakai,K.: "Tissue-specific suppression of aortic fatty acid binding protein in streptozotocin-induced diabetic rats" Eur.J.Biochem.(in press). (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kanda,T.: "Intestinal fatty acid binding protein is available for diagnosis of intestinal ischemia:immunochemical analysis of two patients with ischemic intestinal diseases" Gut. (in press). (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kanda T.: "Intestinal fatty acid binding protein is a useful diagnostic marker for mesenteric infarction" Gastroenterology. (in press). (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Sakakibara,J.: "Molecular cloning and experssion of squalene epoxidase" J.Biol.Chem.270. 17-20 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Wootan,G.M.: "Regulation of fluorescent fatty acid transfer from adipocyte and heart fatty acid binding proteins by acceptor membrane lipid composition and structure" J.Biol.Chem.269. 10517-10523 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Storch J.: "Intestinal lipid digestion and absorption" J.Lipid Res.(in press). (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Herr.F.M.: "Surface lysine residues modulate the collisional transfer of fatty acid from adipocyte fatty acid binding protein to membranes" J.Biol.Chem.(in press). (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Owada,Y.: "Molecular cloning of rat cDNA for cytosolic phospholipase A_2 and the increased gene expression in the dentate gyrus following translent forebrain ischemia" Mol.Brain Res.25. 364-368 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Goto,K.: "Cloning and expression of a cytoskelton-associated diacylglycerol kinase which is dominantly expressed in cerebellum" Proc.Natl.Acad.Sci.,USA. 91. 13042-13046 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Watanabe,K.: "Immunohistochemical distribution of intestinal 15 kDa protein in the human tissue" Arch.Histol.Cytol.(in press). (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Iseki,S.: "Immunohistochemical localization of two types of fatty acid binding proteins in rat ovaries during postnatal development and immature rat ovaries treated with gonadotropins" Anat.Rec.24. 235-243 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Iseki,S.: "Immunohistochemical localization of cyclooxygenase-1 and cyclooxygenase-2 in the rat stomach" Histochem.J.(in press). (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Watanabe, R.: "Molecular cloning of a cDNA encoding a novel fatty acid binding protein from rat skin" Biochem.Biophys.Res.Commun.200. 253-259 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Sakai, K.: "Tissue-specific suppression of aortic fatty acid binding protein in streptozotocin-induced diabetic rats" Eur.J.Biochem.in press. (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kanda, T.: "Intestinal fatty acid binding protein is available for diagnosis of intestinal ischemia : immunochemical analysis of two patients with ischemic intestinal diseases" Gut. in press. (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kanda, T.: "Intestinal fatty acid binding protein is a useful diagnostic marker for mesenteric infarction" Gastroenterology. in press. (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Sakakibara, J.: "Molecular cloning and expression of squalene epoxidase" J.Biol.Chem.270. 17-20 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Wootan, G.M.: "Regulation of fluorescent fatty acid transfer from adipocyte and heart fatty acid binding proteins by acceptor membrane lipid composition and structure" J.Biol.Chem.269. 10517-10523 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Storch, J.: "Intestinal lipid digestion and absorption" J.Lipid Res.(in press). (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Herr, F.M.: "Surface lysine residues modulate the collisional transfer of fatty acid from adipocyte fatty acid binding protein to membranes" J.Biol.Chem.(in press). (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Owada, Y.: "Molecular cloning of rat cDNA for cytosolic phospholipase A_2 and the increased gene expession in the dentate gyrus following translent forebrain ischemia" Mol.Brain Res.25. 364-368 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Goto, K.: "Cloning and expression of a cytoskelton-associated diacylglycerol kinase which is dominantly expressed in cerebellum" Proc.Natl.Acad.Sci., USA. 91. 13042-13046 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Watanabe, K.: "Immunohistochemical distribution of intestinal 15 kDa protein in the human tissue" Arch.Histol.Cytol.(in press). (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Iseki, S.: "Immunohistochemical localization of two types of fatty acid binding proteins in rat ovaries during postnatal development and immature rat ovaries treated with gonadotropins" Anat.Rec.24. 235-243 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Iseki, S.: "Immunohistochemical localization of cyclooxygenase-1 and cyclooxygenase-2 in the rat stomach" Histochem.J.(in press). (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Fujii,H.: "Cloning of a cDNA encoding rat intestinal 15 kDa protein and its tissue distribution" Biochem.Biophys.Res.Commun.190. 175-180 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Watanabe,K.: "Immunohistochemical distribution of heart type fatty acid binding protein:Immunoreactivity in normal human tissues and in acute myocardial infarct" J.Pathol.170. 59-65 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Iseki,S.: "Expression of localization of intestinal 15 kDa protein in the rat" Mol.Cell Biochem.123. 113-120 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Goto,K.: "Molecular clocning and expression of a 90-kDa diacylglycerol kinase that predominantly localizes in neurons" Proc.Natl.Acad.Sci.,U.S.A.90. 7598-7602 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Aono,S.: "Detection of bililubin binding proteins in the liver on a nitrocellulose membrane" Biomed.Res.13. 69-74 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Watanabe.R.: "Molecular cloning of a cDNA encoding a novel fatty acid binding protein from rat skin" Biochem.Biophys.Res.Commun.200 (in-press). (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 小野輝夫: "新生化学実験講座4 脂質I 中性脂質とリポタンパク質" 東京化学同人, 5 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 小野輝夫: "生物薬科学実験講座I-3" 広川書店 (印刷中), (1993)

    • Related Report
      1993 Annual Research Report

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Published: 1993-03-31   Modified: 2016-04-21  

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