| Project/Area Number |
05044204
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| Research Category |
Grant-in-Aid for Overseas Scientific Survey.
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| Allocation Type | Single-year Grants |
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| Research Institution | Department of Agricultural Chemistry, The University of Tokyo |
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Principal Investigator |
ARAI Soichi Department of Agricultural Chemistry, The University of Tokyo, Professor, 農学部, 教授 (20011934)
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| Co-Investigator(Kenkyū-buntansha) |
梁 隆 延世大学校, 工科大学, 教授
FUJIMOTO Daisaburo Faculty of Agriculture, Tokyo University of Agriculture and Technology, 農学部, 教授 (40004288)
HAYASE Fumitaka Department of Agricultural Chemistry, Meiji University, 農学部, 助教授 (80105246)
YANG Ryung College of Engineering, Yensei University
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| Project Period (FY) |
1993
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| Project Status |
Completed (Fiscal Year 1993)
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| Keywords | Maillard reaction / Glycation / 3-Deoxyglucosone / 3-Deoxyfructose / Aldose reductase / 2-Oxoaldehyde reductase / Scavenger receptor / Imidazolone |
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| Research Abstract |
1. Comparison of reaction propagator activity to glycation.
When 3-deoxyglucosone (3DG) and lysozyme were incubated with low- molecular weight compounds in serum of rats, suppressor for reaction between lysozyme and 3DG was observed in the serum.
2. Chemical pathway of propagator formation.
^<14>C-Glucose was orally continuously administered to rats. Serum proteins which were modified with ^<14>C-glucose were detected by HPLC.
3. Identification and distribution of reaction propagator in tissue.
Endogenous 3-deoxyfructose (3DF) which is metabolite of 3DG, was detected and identified in the urine of rats by oral administration of 3DG-free feed.
4. Reaction mechanism of propagator.
R-4-imidazolone, R-4(5H)-imidazolone, R-4(5-hydroxy)-imidazolone and R-4-dihydroxy-2-imidazoline {R=2-(benzoyl-5-pentamide)amino-5-(2,3,4-trihydroxybutyl)} were identified in the reaction system of 3DG and N-alpha-benzoylarginine amide.
5. Self-defense mechanism against glycation.
Aldose reductase was purified and characterized from porcine liver as one of 3DG-metabolizing enzymes. Polyclonal antibody for 2-oxoaldehyde reductase (2-OR) prepared from chicken liver did not show the cross-reactivity against aldehyde reductase I, II and aldose reductase.
When partial sequence of the 2-OR was aligned with aldehyde reductase I and aldose reductase, sequence identities ranged from 20% to 30%.
It was found that 3DG-modified BSA and glycated BSA could be recognized by the type I macrophage scavenger receptor expressed on COS cells, and that the affinity of 3DG-modified BSA for the receptor increased with increasing time of incubation for BSA with 3DG.
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