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Studies on the molecular mechanism of heat shock protein 47.

Research Project

Project/Area Number 05044215
Research Category

Grant-in-Aid for Overseas Scientific Survey.

Allocation TypeSingle-year Grants
Research InstitutionKyoto University

Principal Investigator

NAKAI Akira  Kyoto University, Chest Disease Research Institute, Instractor, 胸部疾患研究所, 助手 (60252516)

Co-Investigator(Kenkyū-buntansha) EL-THAHER Talal  Imperial College, Dept. of Chemistry, Research F
MILLER Andrew  Imperial College, Dept. of Chemistry, Lecturerer
HIRAYOSHI Kazunori  Kyoto University, Chest Disease Research Institute, 胸部疾患研究所, 助手 (80199108)
NAGATA Kazuhiro  Kyoto University, Chest Disease Research Institute, 胸部疾患研究所, 教授 (50127114)
Project Period (FY) 1993
Project Status Completed (Fiscal Year 1993)
KeywordsHeat Shock Protein / Stress Protein / Molecular Chaperone / Structual Analysis / Collagen / Molecular Biology / Intracellnlar transport / Intermolecular Association
Research Abstract

Heat shock protein 47 (HSP47) can bind to collagen and its expression is correlated with that of collagen in many cell lines and tissues. We think that HSP47 has a significant role for procollagen to be secreted correctly. HSP47 exist in the ER and associates transciently with a newly synthesized procollagen. Proteins that facilitate the folding and assembly of polypeptides are called as molecular chaperone. We think HSP47 acts as a molecular chaperone by a substrate-specific manner. Interestingly, the binding of HSP47 with collagen is regulated by a physiological change of pH. In this project, our aims are to reveal the molecular structure of HSP47 and to identify the binding site in collagen.
The progresses of collaboration in this year are listed.
1) The researchers in Japan made the expression vector of HSP47 and sent it to researchers in U.K. They established the purification protocols of HSP47 and now they can get a highly purified HSP47.
2) The researchers in U.K. started the structural analysis of HSP47 by CD spectrum.
3) The crystal structures of some serpin family members are known. Because HSP47 belongs to the serpin family, its structure can be speculated by computer analysis.

Report

(1 results)
  • 1993 Final Research Report Summary
  • Research Products

    (17 results)

All Other

All Publications (17 results)

  • [Publications] A.NAKAI,R.I.Morimoto: "Characterization of a novel chicken heat shock transcription factor,Heat shock factor 3,suggests a new regulatory pathway." Mol.Cell.Biol.13. 1983-1997 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] N.HOSOKAWA,H.TAKECHI,S.YOKOTA,K.HIRAYOSHI&K.NAGATA: "Structure of the gene encoding mouse 47-kDa heat-shock protein(HSP47)." Gene. 126. 187-193 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] M.SATOH,A.NAKAI,Y.SOKAWA,K.HIRAYOSHI&K.NAGATA: "Modulation of the phosphorylation of glucose-regulated protein,GRP78,by transformation and inhibition of glycosylation." Exptl.Cell Res.205. 76-83 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] S.NISHI,W.TAKI,Y.UEMURA,T.HIGASHI,H.KIKUCHI,H.KUDOH,M.SATOH,&K.NAGATA: "Ischemic tolerance due to the induction of HSP70 in a rat ischemic recirculation model." Brain Res.615. 281-288 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] M.YAMAGUCHI,K.HIRAYOSHI,M.OHKUMA&K.NAGATA: "Enhancement of differentiation induction of mouse myelomonocytic leukemic cells by extracellular ATP." J.Cell.Physiol.(In press). (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] H.TAKECHI,N.HOSOKAWA,K.HIRAYOSHI&K.NAGATA: "Alternative 5'splice site selection induced by heat shock." Mol.Cell.Biol.14. 567-575 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] H.KUDO,K.HIRAYOSHI,Y.KITAGAWA,S.IMAMURA&K.NAGATA: "Two collagen-binding proteins,osteonectin and HSP47,are coordinately induced in transformed keratinocytes by heat and other stresses." Exptl.Cell Res.In press.(1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 永田和宏 編: "ストレス蛋白質-基礎と臨床-" 中外医学社, 266 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] K.NAGATA&N.HOSOKAWA: "In hyperthermic Oncology 1992" Taylorand Francis Inc., (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] A.NAKAI & R.I.Morimoto: "Characterization of a novel chicken heat shock transcription factor, heat shock factor 3, suggeses a new regulatory pathway." Mol.Cell.Biol.13. 1983-1997 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] N.HOSOKAWA, H.TAKECHI, S.YOKOTA, K.Hirayoshi & K.NAGATA.: "Structure of the gene encodiry mouse 47-kDa heat-shock protein (HSP47)." Gene. 126. 187-193 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] M.SATOH, A.NAKAI, Y.SOKAWA, K.HIRAYOSHI & K.NAGATA: "Modulation of the phosphorylation of glucose-regulated protein, GRP78, by trans formation and inhibition of glycosylation." Exptl. Cell Res.205. 76-83 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] S.NISHI, W.TAKI, Y.UEMURA, T.HIGASHI, H.KIKUCHI, H.KUDOH, M.SATOH. & K.NAGATA.: "Ischemic tolerance due to the induction of HSP70 in a rat ischemic recirculation model." Brain Res.615. 281-288 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Y.YAMAGUCHI, K.HIRAYOSHI, M.OHKUMA & K.NAGATA: "Enhancement of differentiation induction of mouse myclomonocytic leukemic cells by extracellular ATP." J. Cell Physiol.(in press). (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] H.TAKECHI, N.HOSOKAWA, K.HIRAYOSHI & K.NAGATA: "Alternative 5'splice site selection induced by heat shock." Mol. Cell. Biol.14. 567-575 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] K NAGATA ed.: CHU-GAI IGAKUSHA. Stress Protein, 266 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] K.NAGATA & N.HOSOKAWA: In Hyperthermic Oncology 1992. Flavonoids inhibit the expression of heat shock proteins and the acquisition of thermotolerance ; Inhibition of the activation of heat shock factor is a key mechanism., 57-63 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary

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Published: 1995-02-07   Modified: 2016-04-21  

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