| Project/Area Number |
05045044
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| Research Category |
Grant-in-Aid for international Scientific Research
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| Allocation Type | Single-year Grants |
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| Section | University-to-University Cooperative Research |
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| Research Institution | Chiba University |
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Principal Investigator |
MIYAJI Makoto Research Center of Pathogenic Fungi and Microbial Toxicoses Chiba University, Professor, 真核微生物研究センター, 教授 (40009494)
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| Co-Investigator(Kenkyū-buntansha) |
BAGAGLI Edusado Department of Medicine in Botucutu campus, UNESP Professor, ボツカツ校医学部, 教授
MARQUES Silv パウリスタ総合大学, ボツカツ校医学部, 教授
MENDES Rinal パウリスタ総合大学, ボツカツ校医学部, 教授
MONTENEGRO M パウリスタ総合大学, ボツカツ校医学部, 教授
FRANCO Marcello Department of Medicine in Botucutu campus Universidade Estadual Paulista (UNESP), ボツカツ校医学部, 教授
YOSHIDA Soichi Research Center of Pathogenic Fungi and Microbial Toxicoses' Chiba University, R, 真核微生物研究センター, 助手 (20114340)
TAKEO Kanji Research Center of Pathogenic Fungi and Microbial Toxicoses Chiba University, Pr, 真核微生物研究センター, 教授 (10027097)
FUKUSHIMA Kazutaka Research Center of Pathogenic Fungi and Microbial Toxicoses' Chiba University, A, 真核微生物研究センター, 助教授 (90114321)
NISHIMURA Kazuko Research Center of Pathogenic Fungi and Microbial Toxicoses Chiba University, Pr, 真核微生物研究センター, 教授 (00114314)
MARQUES Silvio Alencar Department of Medicine in Botucutu campus, UNESP Professor
MENDES Rinaldo Ponceo Department of Medicine in Botucutu campus, UNESP Professor
MONTENEGRO Mario R.G. Department of Medicine in Botucutu campus, UNESP Professor
SILVIO Alenc パウリスタ総合大学, ボツカツ校医学部, 助教授
EDUARDO Baga パウリスタ総合大学, ボツカツ校医学部, 助教授
RUNALDO Ponc パウリスタ総合大学, ボツカツ校医学部, 助教授
RUBENS G. Mo パウリスタ総合大学, ボツカツ校医学部, 名誉教授
MARCELLO Fra パウリスタ総合大学, ボツカツ校医学部, 教授
宇野 潤 千葉大学, 真核微生物研究センター, 助手 (40114243)
寺尾 清 千葉大学, 真核微生物研究センター, 教授 (30009120)
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| Project Period (FY) |
1993 – 1995
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| Project Status |
Completed (Fiscal Year 1995)
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| Budget Amount *help |
¥6,000,000 (Direct Cost: ¥6,000,000)
Fiscal Year 1995: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1994: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1993: ¥2,000,000 (Direct Cost: ¥2,000,000)
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| Keywords | Brazil / Paracoccidioides brasiliensis / Paracoccidioidomycosis / Ecology / Imported mycosis / Pathogenic dematiaceous fungi / Dimorphic fungus |
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| Research Abstract |
Paracoccidioidomycosis, caused by the thermal dimorphic fungus Paracoccidioides brasiliensis, is systemic human mycosis geographically confined to Latin America. The natural habitat of P.brasiliensis is still unclear. Since 1993 to study this mycosis we have done University-to-University cooperative Research with Botucutu Campus of Universidade Estadual Paulista (UNESP), Brasil. The university hospital of UNESP in Botucutu receives patients with paracoccidioidomycosis from the endemic area. Because a culture medium with high plating efficiency for P.brasiliensis is of critical importance for isolation of the fungus from natural environments, efforts have been made to improve the plating efficiency of culture medium and we found that when a water extract of yeast phase cells P.brasilienses was added in combination withhorse serum to brain heart infusion agar (BHIA), the plating efficiency was highly improved. Using this medium we tried to isolate the causative agent from nature. About 800 samples of soil from various areas were gathered and cultured. Among them five yeast like colonies which resembled P.brasiliensis were obtained. Microscopically these five isolates formed chlamydospores on SDA and converted from mycelial to yeast at 35 C on BHIA.Multiple budding were also seen. We also obtained ninety-eight black colonies from isolation of pathogenic dematiaceous fungi. They were Curvularia lunata, Exophiala spinifera, exophiala spp, Fonsecaea pedrosoi, Phialophora verrucosa, Ramichloridium anceps, Rhinocladiella atrovirens and Veronea botyosa. 581 strains of Aspergillus spp. were isolated from 887 samples collected.
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