| Project/Area Number |
05268101
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| Research Category |
Grant-in-Aid for Scientific Research on Priority Areas
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| Allocation Type | Single-year Grants |
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| Research Institution | Kyoto University, Chest Disease Research Institute |
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Principal Investigator |
NAGATA Kazuhiro Kyoto University, Chest Disease Research Institute, Professor, 胸部疾患研究所, 教授 (50127114)
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| Co-Investigator(Kenkyū-buntansha) |
ENDOH Toshiya Nagoya University, Graduate School of Science, Professor, 理学部, 教授 (70152014)
MORI Masataka Kumamoto University, Faculty of Medicine, Professor, 医学部, 教授 (40009650)
KOHNO Kenji Nara Institute of Science and Technology, Professor, 遺伝子教育研究センター, 教授 (50142005)
MIKI Kunio Kyoto University, Graduate School of Science, Professor, 理学部, 教授 (10116105)
KUWASHIMA Kunihiro University of Tokyo, Graduate School of Science, Assistant Professor, 大学院・理学系研究科, 助教授 (70091444)
米田 悦啓 大阪大学, 医学部, 教授 (80191667)
瀬野 悍二 国立遺伝学研究所, 変異遺伝研究部門, 教授 (30076989)
堀内 賢介 国立遺伝学研究所, 微生物遺伝研究部門, 教授 (70219210)
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| Project Period (FY) |
1995
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| Project Status |
Completed (Fiscal Year 1995)
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| Budget Amount *help |
¥278,000,000 (Direct Cost: ¥278,000,000)
Fiscal Year 1995: ¥99,000,000 (Direct Cost: ¥99,000,000)
Fiscal Year 1994: ¥89,000,000 (Direct Cost: ¥89,000,000)
Fiscal Year 1993: ¥90,000,000 (Direct Cost: ¥90,000,000)
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| Keywords | stress proteins / heat shock proteins / molecular / chaperones / stress response / HSP47 / HSP70 / 核輸送 |
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| Research Abstract |
Experiment of antisense transfection was performed to elucidate the function of HSP47, a collagen-specific molecular chaperone. Stable transfectant expressing the antisense RNA of HSP47 were found to synthesize reduced amounts of type I collagen as well. When cDNA encoding alpha1 (I) collagen gene was transfected into these cell lines, overexpressed collagen was recovered in the detergent-insoluble fraction, suggesting the chaperone function of HSP47 which maintain the nascent polypeptide of collagen to be soluble in the endoplasmic reticulum.
Molecular mechanism on the recognition of target proteins by GroEL was analyzed using alpha-lactalbumin (alpha-LA). Dissociation constants, effect of ioninc strength and rate constants of the interaction of GroEL with alpha-LA in the reduced state as well as in the moleten globule state were determined.
Two independent pathways of the transports of nascent proteins into mitochondria were established : MSF-dependent pathway where nascent proteins are targeted to membrane receptors via the function of MSF,and MSF-independent pathway where proteins are directly handled to Mas20/Mass22 receptor on the mitochondrial membrane.
A novel membrane protein, Rer1p, was found in the golgi membrane. Rer1p was suggested to be involved in the quality control mechanism since the incorrectly transported proteins to the Golgi are returned back to the endoplasimic reticulum by the function of this protein.
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