| Project/Area Number |
05268102
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| Research Category |
Grant-in-Aid for Scientific Research on Priority Areas
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| Allocation Type | Single-year Grants |
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| Research Institution | Tokyo Metropolitan Organization for Medical Research |
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Principal Investigator |
YAHARA Ichiro The Tokyo Metropolitan Institute of Medical Science, Vice-Director, 副所長 (60109957)
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| Co-Investigator(Kenkyū-buntansha) |
SEKIMIZU Kazuhisa Kyushu Univ., Fac.Pharmaceutical Sci., Professor, 薬学部, 教授 (90126095)
NAKAMURA Yoshikazu Univ.Tokyo, Inst.Med.Res., Associate Professor, 医科学研究所, 助教授 (40114590)
SHINAGAWA Hideo Osaka Univ., Res.Inst.Microbial Diseases., Professor, 微生物病研究所, 教授 (40029799)
ITO Koreaki Kyoto Univ., Inst.Virus Res., Professor, ウイルス研究所, 教授 (90027334)
ISHIHAMA Akira Nat.Inst.Genetics, Dept.Mol.Genetics, Professor, 分子遺伝, 教授 (80019869)
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| Project Period (FY) |
1993 – 1995
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| Project Status |
Completed (Fiscal Year 1996)
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| Budget Amount *help |
¥278,000,000 (Direct Cost: ¥278,000,000)
Fiscal Year 1995: ¥94,000,000 (Direct Cost: ¥94,000,000)
Fiscal Year 1994: ¥94,000,000 (Direct Cost: ¥94,000,000)
Fiscal Year 1993: ¥90,000,000 (Direct Cost: ¥90,000,000)
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| Keywords | stress response / stress protein / heat shock protein / molecular chaperone / oxidative stress / SOS response / transcription factor / proteasome / 細胞骨格 / 転写制御 / 蛋白質の分泌 / タンパク質の分泌 |
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| Research Abstract |
1) Molecular chaperone function of HSP90, a major stress protein, has been clarified..
2) Molecular structure of RuvC,an endonuclease induced by the SOS response which recognizes intermediate structures of DNA recombination has been determined by X-ray crystallography. Function of RuvC was also anlyzed.
3) Subunit proteins of 26S and 20S proteasomes have been identified. Their cDNA's have been isolated and their functions have been extensively analyzed.
4) sigma32, a stress-specific transcription factor, has been shown to be quantitatively regulated by stimulation of translation and its stabilization.
5.A new protease, FtsH,has been discovered and shown to belong to the AAA family. In addition, FtsH has been shown to degradate sigma32.
6.Intermediate structures of substrate proteins recognized by chaperonin have been investigated.
7.Relaxation mechanism of supercoil structure of plasmid DNA upon heat shock has been investigated.
8.It was found that intranuclear ongogenic product Myb interacts with the heat shock factor HSF3.
9.Signal transduction mechanism involved in the activation of NF-kB upon stress response has been elucidated.
10.Processing and cell surface expression of Hsp65 in macrophage has been investigated upon paracyte infection.
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