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Molecular Mechanism of Stress Response

Research Project

Project/Area Number 05268103
Research Category

Grant-in-Aid for Scientific Research on Priority Areas

Allocation TypeSingle-year Grants
Research InstitutionThe Tokyo Metropolitan Institute of Medical Science

Principal Investigator

YAHARA Ichiro  The Tokyo Metropolitan Institute of Medical Science, Vice-Director, 副所長 (60109957)

Co-Investigator(Kenkyū-buntansha) YOSHIDA Masasuke  Tokyo Inst.Tech., Res.Lab.Resources Utilization, Professor, 資源化学研究所, 教授 (90049073)
ISHIHAMA Akira  Nat.Inst.Genetics, Dept.Mol.Genetics, Professor, 分子遺伝, 教授 (80019869)
KAGAWA Yasuo  Jichi Med.School, Professor, 医学部, 教授 (30048962)
HORIUCHI Kensuke  Nat.Inst.Genetics.Dept.Cell.Genetics.Professor, 細胞遺伝, 教授 (70219210)
NAGATA Kazuhiro  Kyoto University, Inst.Chest Diseases, Professor, 胸部疾患研究所, 教授 (50127114)
深沢 俊夫  慶応義塾大学, 医学部, 教授 (90029934)
Project Period (FY) 1993 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥61,200,000 (Direct Cost: ¥61,200,000)
Fiscal Year 1996: ¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 1995: ¥18,000,000 (Direct Cost: ¥18,000,000)
Fiscal Year 1994: ¥18,000,000 (Direct Cost: ¥18,000,000)
Fiscal Year 1993: ¥22,200,000 (Direct Cost: ¥22,200,000)
Keywordsstress response / stress protein / heat shock protein / molecular chaperone / oxidative stress / SOS response / protein folding / intracellular transport of proteins
Research Abstract

1) Molecular chaperone function of HSP90, a major stress protein, has been clarified..
2) Molecular structure of RuvC,an endonuclease induced by the SOS response which recognizes intermediate structures of DNA recombination has been determined by X-ray crystallography. Function of RuvC was also anlyzed.
3) Subunit proteins of 26S and 20S proteasomes have been identified. Their cDNA's have been isolated and their functions have been extensively analyzed.
4) sigma32, a stress-specific transcription factor, has been shown to be quantitatively regulated by stimulation of translation and its stabilization.
5.A new protease, FtsH,been discovered and shown to belong to the AAA family. In addition, FtsH has been shown to degradate sigma32.
6.Functions of the collagen-specific molecular chaperone, HSP47, have been elucidated.
7.A chaperonin specific for insect endosymbiosis (symbionin) has been identified and characterized. In addition, Its cDNA was cloned and its expression was confirmed.
8.Intermediate structures of substrate proteins recognized by chaperonin have been investigated.
9.The mitochondria-transport stimulating factor, MSF,has been newly identified and characterized. Its cDNA has been cloned and its expression was examined with a variety of cell types.

Report

(4 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • 1993 Annual Research Report
  • Research Products

    (37 results)

All Other

All Publications (37 results)

  • [Publications] Nagata,K.: "HSP47,a collagen-specific molecular champerone in the endoplasmic reticulum." TIBS. 21. 23-26 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Shinagawa,H.and Iwasaki,H.: "Processing the Holliday junction in homologous recombination." TIBS. 21. 107-111 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Mihara,K.and Omura,T.: "Cytoplasmic chaperones in precursor targeting to mitochondria:the role of MSF and hsp70" Trends in Cell Biology. 6. 104-108 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Hatanaka,H.et al.: "Tertiary structure of destrin and structural similarity between two actin-regulating protein families." Cell. 85. 1047-1055 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kimura,Y.et al.: "Role of the protein chaperone YDJ1 in establishing Hsp90-mediated signal transduction pathways." Science. 268. 1362-1365 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Tomoyasu,T.et al.: "Escherichia coli FtsH is a membrane-bound,ATP-dependent protease which degrades the heat-shock transcription factor σ32." EMBOJ.14. 2551-2560 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Nagata, K.: "HSP47, a collagen-specific molecular champerone in the endoplasmic reticulum." TIBS. 21. 23-26 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Shinagawa, H.: "Processing the Holliday junction in homologous recombination." TIBS. 21. 107-111 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Mihara, K.: "Cytoplasmic chaperones in precursor targeting to mitochondria : the role of MSF and hsp70." Trend in Cell Biology. 6. 104-108 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Hatanaka, H.: "Tertiary structure of destrin and structural similarity between two actin-regulating protein families." Cell. 85. 1047-1055 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kimura, Y.: "Role of the protein chaperone YDJ1 in establishing Hsp90-mediated signal transduction pathways." Science. 268. 1362-1365 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Tomoyasu, T.: "Escherichia coli FtsH is a membrane-bound, ATP-dependent protease which degrades the heat-shock transcription factor sigma^<32>." EMBO J.14. 2551-2560 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kimura, Y.: "Role of the protein chaperone YDJ1 in establishing Hsp90-mediated signal transduction pathways." Biochemistry. 34. 8123-8129 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Jeon, Y.H.: "Solution structure of the C-terminal domain of RNA polymerase alpha subunit responsible for the contact with trans-acting transcription factors and cis-acting UP element of promoter." Seience. 270. 1945-1997 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kihara, A.: "FtsH is required for proteolytic elimination of uncomplexed forms of Sec Y,an essential protein translocase subunit." Proc.Natl.Acad.Sci.USA. 92. 4532-4536 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Saito, A.: "Identification of four acidic aminoacids that constitute the catalytic center of the RuvC Holliday junction resolvase." Proc.Natl.Acad.Sci.USA. 92. 7470-7474 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kominami, K.: "Nin 1p, a regulatory subunit of the 26S proteasome, is necessary for activation of Cdc28p kinase of Saccharomyces cerevisiae." EMBO J.14. 3105-3115 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Gotoh, Y.: "Involvment of the MAP kinase cascade in Xenopus mesoderm induction." EMBO J.14. 2491-2498 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Imamoto N.: "In vivo evidence for involvment of a 58kDa component of nuclear pore-targeting complex in nuclear protein import." EMBO J.14. 3617-3626 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Nagata,K.: "HSP47,a collagen-specific molecular champerone in the endoplasmic reticulum." TIBS. 21. 23-26 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Shinagawa,H.and Iwasaki,H.: "Processing the Holliday junction in homologous recombination." TIBS. 21. 107-111 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Mihara,K.and Omura,T.: "Cytoplasmic chaperones in precursor targeting to mitochondria : the role of MSF and hsp70" Trends in Cell Biology. 6. 104-108 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Hatanaka,H. et al.: "Tertiary structure of destrin and structural similarity between two actin-regulating protein families." Cell. 85. 1047-1055 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Kimura,Y.et al.: "Role of the protein chaperone YDJ1 in establishing Hsp90-mediated signal transduction pathways." Science. 268. 1362-1365 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] Tomoyasu,T.et al: "Escherichia coli FtsH is a membranl-bound, ATP-dependent protease which degrades the heat-shock transcription factor σ^<32>." EMBOJ.14. 3617-3626 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] Yonehara, M. et al.: "Heat-induced chaperone activity of HSP90." J. Biol. Chem.271. 2641-2645 (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] Higashitani, N. et al.: "SOS induction in Escherichia coli by single-stranded DNA of mutant filamentous phage : Monitoring by cleavage of LexA repressor." J. Bacteriol.177. 3610-3612 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Nagata, K.: "HSP47: a collagen-specific molecular chaperone in the endoplasmic reticulum." TIBS. 21. 23-26 (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] Murai, N. et al.: "Kinetic analysis of interactions between GroEL and reduced α-lactalbumin." J. Biol. Chem.270. 19957-19963 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Nakai, A. et al.: "The DNA-binding properties of two heat shock factors, HSF1 and HSF3, are induced in the avian erythroblast cell line HD6." Mol. Cell Biol.15. 5268-5278 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Kimura, Y. et al.: "Role of the protein chaperone YDJ1 in establishing Hsp90-mediated signal transduction pathways." Science. 268. 1362-1365 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Sakurai,H.: "Yeast GAL11 protein is a distinctive type transcription factor that enhances basal transcription in vitro" Proc.Natl.Acad.Sci,USA. 90. 8382-8386 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Yuzawa,H.: "Heat induction of sigma-32 synthesis mediated by mRNA secondary structure:a primary step of the heat shock response in Escherichia coli." Nucleic Acid Res.21. 5449-5455 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Morioka,M.: "Chaperonin produced by an intracellular symbiont is an energy-coupling protein with phosphotransferase activity." J.Biochem.114. 246-250 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Ishihama,A.: "Protein-protein communication within the transcription apparatus." J.Bacteriol.175. 2483-2489 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Minami,Y.: "The calmodulin-binding domain of the mouse 90-kDa heat shock protein." J.Biol.Chem.268. 9604-9610 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Kimura,Y.: "Temperature-sensitive mutants of hsp82 of the budding yeast,Saccharomyces cerevisiae." Mol.Gen.Genet.242. 517-527 (1994)

    • Related Report
      1993 Annual Research Report

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Published: 1993-04-01   Modified: 2019-02-28  

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