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糖鎖遺伝子とその生物機能

Research Project

Project/Area Number 05274105
Research Category

Grant-in-Aid for Scientific Research on Priority Areas

Allocation TypeSingle-year Grants
Research InstitutionHokkaido University (1994-1997)
Jichi Medical University (1993)

Principal Investigator

斎藤 政樹 (斉藤 政樹)  北海道大学, 医学部, 教授 (60012762)

Co-Investigator(Kenkyū-buntansha) 岩森 正男  東京大学, 医学部, 助教授 (90110022)
小川 智也  東京大学, 農学部, 教授 (30087572)
入村 達郎  東京大学, 薬学部, 教授 (80092146)
鈴木 明身  東京都臨床医学総合研究所, 部長 (70134533)
谷口 直之  大阪大学, 医学部, 教授 (90002188)
関谷 剛男  国立がんセンター研究所, 部長 (70142651)
Project Period (FY) 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥117,100,000 (Direct Cost: ¥117,100,000)
Fiscal Year 1997: ¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 1996: ¥22,900,000 (Direct Cost: ¥22,900,000)
Fiscal Year 1995: ¥30,200,000 (Direct Cost: ¥30,200,000)
Fiscal Year 1994: ¥31,000,000 (Direct Cost: ¥31,000,000)
Fiscal Year 1993: ¥30,000,000 (Direct Cost: ¥30,000,000)
Keywords糖鎖遺伝子 / 重点領域研究 / 国際シンポジウム / 糖鎖の生物機能 / 糖鎖複合体 / バイオサイエンス / 糖鎖合成酵素遺伝子 / 糖鎖遺伝子クローニング / 増殖・分化 / 糖鎖発現の制御因子 / 分子認識と接着 / 糖鎖複合体の再構成 / 糖転移酵素遺伝子 / 糖鎖機能 / 糖鎖複合体の分子設計 / 糖転移酵素
Research Abstract

平成5〜8年度の4年間支援を受けた本重点研究を終了するに当って、得られた研究業績を取りまとめ、平成9年10月20日に開催された「研究終了ヒアンング」において、科学研究費補助金重点領域研究に係わる経過等の報告書「糖鎖遺伝子とその生物機能(領域番号:336・糖鎖遺伝子)」として提出し内容説明した。同時に本成果報告書を増刷し、班員・班友及びこの領域に関心を寄せる人々に対しては勿論のこと、他分野の第一線の研究者並びに研究指導者の方々の本学問領域に対する、より深い理解と支援を得るため、配布し率直なる批判・意見を受けた。平成2年以来、多彩な生物機能が注目されるようになった「糖鎖複合体」の「糖鎖」を取り上げ、主とレて糖鎖合成酵素遺伝子の分子生物学的な構造解析と発現制御という観点から、遺伝子レベルでの糖鎖機能を総合的に解析することにより、新しいバイオサイエンスの分野を積極的に確立しようとする本研究分野並びに関連分野は、未来を志向した先導的な我が国の重点研究分野として、文部省、科学技術庁、厚生省、通産省、農水省の支援を得て、その育成が行われて来ているが、主として基礎研究に焦点をしぼった文部省支援の本重点領域研究は、国際的視点からも実に時宜を得たものであり、とくに「糖鎖遺伝子」の研究領域における本研究班員・班友の国際舞台での活曜は飛曜的に高まり、国際的な本研究分野の新展開における我が国の貢献度は極めて大きなものがあった。さらに本研究分野で国際的に先端的研究を推進している海外の研究者3名(米国のR.Ledeen教授、ポーランドのJ.Kdscielak教授、イスラエルのA.Futerman博士)を共同研究者として招待して、平成9年12月9日に経団連ホールで開催した国際シンポジウムInternational Symposium on ″Glycogenes and Biological Functions of Their Productsには、300名を超える予想以上の多くの研究者の参加を得て、活発な討義と共に、4年間の研究業績評価並びに本研究分野の将来が展望できたものと確信している。

Report

(5 results)
  • 1997 Annual Research Report
  • 1996 Annual Research Report
  • 1995 Annual Research Report
  • 1994 Annual Research Report
  • 1993 Annual Research Report
  • Research Products

    (36 results)

All Other

All Publications (36 results)

  • [Publications] Nakamura,M., Saito,M., et al.: "Rapid Internalization of Exogenous Ganglioside GM3 and Its Metabolism to Ceramide in Human Myelogenous Leukemia HI-60 Colls" FEBS Lett.400. 350-354 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Nakamura,M., Saito,M., et al.: "CMP-NeuAc:Gal β1→4GlcNAcα2→6Sialyltransferase Catalyzes NeuAc Transfer to Glycolipids" J.Lipid Res.38. 1795-1806 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Ohta,M., Saito,M., et al.: "Suppression of Hematopoietic Activity in Tenascin-C-Deficient Mice" Blood,. (in press)(June 1). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Ishii,A., Saito,M., et al.: "Expression Cloning and Molecular Characterization of Human Ganglioside GM3 Synthase (Sialytransferase-1 CMP-N-acethylneuraminic" Nature. in revision. (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Mei Xu, Saito,M., et al.: "A Severe Clinical Phenotype of Maroteaux-Lamy Syndrome Caused by A Nonsense Point Mutation in Arylsulfatase B Gene" Human Genet.(in press). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] 斎藤政樹: "糖鎖生物学(蛋白質核酸酵素増刊号)" 共立出版株式会社(印刷中), 250 (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] 斎藤政樹: "医科分子生物学 改訂第3版(村松正美、谷口維紹編)" 南江堂, 501 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Saito. M,: "Purification and Characterization of Heparan Sulfate 2-Sulfotransferase from Cultured Chinese Hamster Ovary Cells" J. Biol. Chem.271. 7645-7653 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Saito M: "Interleukin-3-associated ganglioside GDla is induced independently of normal interleukin-3 receptor in murine myelogenous leukaemia NFS60 cells transfected with the interleukin-3 gene" Glycoconjugate J.13. 255-261 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Teniguchi N: "Transcriptional regulation of the N-acetylglucosaminyltransferaseV gene in human bile duct carcinoma cells (HuCC-T1) is medicated by ets-1." J. Biol. Chem. 271. 26706-26712 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Suzuki A: "Expression of cholara toxin B subunit in the golgi apparatus of swiss 3T3 cells inhibits DNA synthesis induced by basic fibroblast growth factor." J. Biochem.,. 119. 985-990 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Irimura T: "Molecular cloning and expression of cDNA encoding human macrophage C-type lectin. Its unique carbohydrate binding specificity for Tn antigen." J. Immunol.156. 128-135 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Ogawa T.: "Synthesis of a novel glycosaminoglycan pentasaccharide serine having an N-acetylgalactosamine residue α-linked to the linkage tetrasaccharide." Glycoconjugate J.13. 933-936 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 斎藤政樹: "医科分子生物学改訂第3版(松村正實、谷口維紹編集)" 南江堂, 501 (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Tsunoda A, Nakamura M, Kirito K, Hara K, Saito M: "IL-3-associated Expression of Gangliosides in Mouse Myclogenous Leukemia NFS60 Cells Introduced with IL-3 Gene:Expression of Ganglioside GDla and A Key.Involvement of CMP-N.euAc:Lactosylceramide α2,6-Sialyltransferase in GDla Expression." Biochemistry. 34. 9356-9367 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Terui Y, Furukawa Y, Ohta M, Sakoe K, Kitagawa S, Saito M: "Apoptosis during HL-60 Cell Differentiation is Closely Related to a G0/Gl Cell Cycle Arrest." J.Cell.Physiol. 164. 74-84 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Miyoshi E, Hayashi N, Fusamoto H, Kamada T, Taniguchi N: "Transfection of N-acetylglucosaminyltransferase III gene suppresses expression of hepatitis B virus in a human hepatoma cell line,HB611." J.Biol.Chem.270. 28311-28315 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Kawano T, Koyama S, Takematsu H, Kozutsumi Y, Kawasaki H, Kwashima S, Kawasaki T, Suzuki A: "Molecular cloning of cytidine monophospho-N-acetylneuraminic acid hydroxylase.Regulation of species-and tissue-specific expression of N-glycolylneuraminic acid." J.Biol.Chem.270. 16458-16463 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Kimura T, Imai Y, Irimura T: "Calcium-dependent conformation of a mouse macrophage calcium-type lectin.Carbohydrate binding activity is stabilized by an antibody specific for a calcium dependent opitope." J.Biol.Chem.270. 16056-16062 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Kitagawa H, Tanaka Y, Tsuchida K, Goto F, Ogawa T, Lidholt K, Lindahl U, Sugahara K: "N-Acetylgalactosamine(GalNAc)transfer to the common carbohydrate-protein linkage region of sulfated glycosaminoglycans." J.Biol.Chem.270. 22190-22195 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 斎藤政樹: "「癌細胞の分化誘導とアポトーシス-癌分 化誘導療法」(穂積本男、斎藤政樹、佐藤光信 編)" 共立出版(株)(東京), 257 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 斎藤政樹: "ガングリオシド研究法I,II-生物化学実験法35(鈴木康夫、安藤進編著)" (株)学会出版センター, 521 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Sakai T,Kawakatsu H,Ohta M,Saito M: "Tenascin Induction in Tenascin Non-producing Carcinoma Cell Lines in Vivo and by TGF-β1 in Vitro." J.Cell.Physiol.159. 561-572 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Miyoshi E, Nishikawa A,Ihara A,Hayashi N,Fusamoto H,Kamada T,Taniguchi N: "Selective suppression of N-acetylglucosaminyl transferase III activity in a human hepatoblastoma cell line transfected with hepatitis B virus" Cancer Res.54. 1854-1858 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Kawano T,Kozutsumi Y,Kawasaki T,Suzuki A: "Biosynthesis of N-glycolylneuraminic acid-containing glycoconjugates.Purification and characterization of the key enzyme of the cytidine monophospho-N-acetylneuraminic acid hydroxylation system." J.Biol.Chem.269. 9024-9029 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Furukawa Y,Ohta M,Terui Y,Sakoe K,Kitagawa S,Saito M: "The Role of Cellular Transcription Factor E2F in the Regulation of cdc2 mRNA Expression and Cell Cycle Control of Human Hematopoietic Cells." J.Biol.Chem.269. 26249-26258 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Shirotani K,Taylor-Papadimitriout J,Gendler SJ,Irimura T: "Transcriptional regulation of MUC1 gene in colon carcinoma cells by a soluble factor:Identification of a regulatory element." J.Biol.Chem.269. 15030-5035 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Nunomura S,Matsuzaki Y,Ito.Y, Ogawa T: "Experiments directed towards synthesis of complex glycosphingolipids:Ganglio-ganglioside GQ1b." Pure & Appl.Chem.66. 2123-2126 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 斉藤政樹: "糖鎖 I.糖鎖と生命(永井克孝、斉藤政樹、谷口直之、鈴木明身ら編著) 第1部 糖鎖は増殖と分化をコントロールする" 東京化学同人(東京), 30 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 斉藤政樹: "エイジングの科学-21世紀の老化研究(積田 亨編)(現代化学増刊)" 東京化学同人(東京), 28 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Sakai.T.,Kawakatus.H.,Hirota,N.,et al.: "Tenascin Expression in vitro and vivo: Comparison between Epithelial and Nonepithelial Rat Cell Lines." Exp,Cell Res.206. 244-254 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] lhara,Y.,et al.: "ol)NA cloning,expression,and chromosomal localizaition of human N-acetyl glucosaminyl-transferase III(GnT-III)." J.Biochem.113. 692-698 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Furukawa,K.,et al.: "GD2 ganglioside on HTLV-I infected T cells: possible activation of acetylgalactosaminyltransferase gene by p40^<tux>" Proc.Natl.Acad.Sci,USA.90. 1972-1976 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Hasimoto,Y.,et al.: "Purification and characterization of UDP-N-acetylgalactosamine GM3/GD3 N-acetylgalactosaminyltransferase (EC2.4.1.92)from mouse liver." J.Biol.Chem.268. 25857-25864 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Dohi,D.F.,et al.: "Regulation of sialomucion in colon carcnoima cells." J.Biol.Chem.268. 10133-10138 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Dabrowski,zu.et al.: "Solution conformation of the tetrasaccharide glycoside Xylbeta1-(Manalpha1-3)2Manbeta1-4Glcbeta1R from the mollu-series alycosphingolipids" Biochem.Biophys.Res.Commun.192. 1057-1065 (1993)

    • Related Report
      1993 Annual Research Report

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Published: 1993-04-01   Modified: 2016-04-21  

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