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"Man-Earth System : A scientific approach to realize human society in harmony with the Earth"-Molecular Breeding of Plants Resistant to Nutrient Stress-Isolation of Plant Genes Responsible for Stress Resistance-

Research Project

Project/Area Number 05278105
Research Category

Grant-in-Aid for Scientific Research on Priority Areas

Allocation TypeSingle-year Grants
Research InstitutionUniversity of Tokyo

Principal Investigator

CHINO Mitsuo  University of Tokyo, Graduate School of Agricultural and Life Sciences, Professor, 大学院・農学生命科学研究科, 教授 (10007677)

Co-Investigator(Kenkyū-buntansha) SANO Hiroshi  Kara High Technology Graduate School University, Genes Educational Research Center, Professor, 遺伝子教育研究センター, 教授 (20178809)
SEKIYA Jiro  Kyoto University, Graduate School of Agricultural Sciences, Professor, 大学院・農学研究科, 教授 (10035123)
MATSUMOTO Hideaki  Okayama University, Research Institute for Bioresources, Professor, 資源生物科学研究所, 教授 (80026418)
小畑 仁  三重大学, 生物資源学部, 助教授 (70024594)
但野 利秋  北海道大学, 農学部, 教授 (40001440)
米田 好文  北海道大学, 理学部, 教授 (10124215)
Project Period (FY) 1993 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥100,800,000 (Direct Cost: ¥100,800,000)
Fiscal Year 1997: ¥15,700,000 (Direct Cost: ¥15,700,000)
Fiscal Year 1996: ¥16,500,000 (Direct Cost: ¥16,500,000)
Fiscal Year 1995: ¥21,000,000 (Direct Cost: ¥21,000,000)
Fiscal Year 1994: ¥23,800,000 (Direct Cost: ¥23,800,000)
Fiscal Year 1993: ¥23,800,000 (Direct Cost: ¥23,800,000)
KeywordsHigher plants / Resistance to nutrient stress / Food production / Genes / Transgenic plants / 重金属 / アルミニウム / イオウ / メタロチオネイン / リン酸欠乏 / フィトケラチン / 酸性フォスファターゼ / Al,Cu,Cd,P,S,Kストレス
Research Abstract

Studies toword molecular breeding of nutrient stress tolerant plants have been carried out in this investigation in order to increase the world food supply without significant inpacts on the environment. The nutrient stresses studied were Cu, Al, and S stresses. The results obtained in 1997 can be summarized as follows : 1) Metallothionein in Arabidopsis thaliana is shown involved in the regulation of cellular differenciation in particular types of cells (Chino), 2) Two kinds of cDNAs were induced from yeast under Al excess and the DNA sequences of these genes has revealed that one of the genes is coding heat shock inducing HSP150 gene and the other is coding membrane protein gene Sed1 Matsumoto), 3) APS sulfotransferase which has been found to be a key enzyme in S assimilation in plants has been purified and the isolation of cDNA of the enzyme protein has been tried by complementation method using yeast mutant lacking in APS kinase and PAPS reductse (Sekiya), 4)Function of cystein synthetase has been clarified and the enhance of the gene expression may have made the transformant plants tolerant to hydrogen sulfide. Functional analysis of genes involved in sulfur assimilation were carried out (SANO).

Report

(6 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • 1995 Annual Research Report
  • 1994 Annual Research Report
  • 1993 Annual Research Report

Research Products

(33 results)

All Other

All Publications

  • [Publications] 「人間地球系」総括班: "平成9年度研究成果報告"「人間地球系」研究広報. 61-61 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Man-Earth Project Member: "1997 Research Results Report"61 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yamashita,K.: "Enhancement of nitrate efflux and the plasma membrane hydrogen ion-transport activity of barley roots by nitrate treatment." J.Plant Nutr.20・6. 657-668 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Ma,J.F.: "Internal detoxification mechanism of Al in Hydrangea.Identification of Al form in the leaves." Plant Physiol.113・4. 1033-1039 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Ma,J.F.: "Detoxify aluminum with buckwheat." Nature.390・11. 569-570 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Yamamoto,Y.: "Oxidative damage to membranes by a combination of aluminum and iron in suspension-cultured to bacco cells." Plant Cell Physiol.38・12. 1333-1339 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Ara,T.: "Non-radioactive adenosine 5'-phosphosulfate sulfotransferase assay by coupling with sulfite reductase and O-acetylserine(thio)lyase." Biosci.Biotech.Biochem.61・4. 621-624 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Nakamura,T.: "Molecular characterization of atcs26,a gene encoding a novel cysteine synthetase from Arabidopsis thaliana." Plant Physiol.114・4. 747 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Yamamoto,Y.: "Tolerance to aluminum acquired during phosphate starvation of cultured tobacco cells." Plant Physiol.112.1. 217-227 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Ma,J.F.: "Al-induced inhibition of root elongation in corn,Zea mays L.is overcome by Si addition." Plant Soil. (in press). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Ma,J.F.: "Internal detoxification mecahnism of aluminum in Hydrangea macrophylla.Identification of Al from in the leaves." Plant Physiol. (in press). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Sasaki,M.: "Aluminum inhibits growth and stability of cortical microtubules in wheat(Triticum aestivum)roots." Soil Sci.Plant Nutr.(in press). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Ara,T.: "Non-radioactive adenosine 5'-phosphsulfate sulfotransferase assay by coupling with sulfite reductase and O-acetylserine(thiol)lyase." Biosci.Biotech.Biochem.,. (in press). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Yamaguchi,Y.: "Isolation and characterization of a cDNA encoding a sulfate transporter from Arabidopsis thaliana." Plant Physiol.(in press). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] P.Chongpraditnun: "The Induction of cytosolic Cu, Zn-superoxide dismutase in soybean roots by coppet" Environmental Geochemistry and Health. 16. 515-524 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] P.Chongpraditnun: "Studies on possible detection of mammalian mctallothioeiin in higher plant by radioimmunoassay." Thai Journal of Soils and Fertilizcrs. 16. 235-240 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] Matsumoto,H: "Response of the plant root to aluminum stress: analysis of the inhibition of the root elongation and changes in membranc function." J. Plant Res.(in press). (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] Sasaki,M.: "Lignin deposition induced by aluminum in wheal(Triticum aestivum)roots." Physiol. Plant. (in press). (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] Ozawa,K.: "Purification and propertics of acid phosphatase secreled from lupin under phosphorus-deficiency conditions." Soil Sci.Plant Nutr.41. 461-469 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Obata,H.: "Effcct of Cd on plasma membrane ATPase from plant roots differing in tolerance to Cd." Soil Sci. Plant Nu.(in press). (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] M.Y.Hirai et al.: "Differential regulation of soybean seed storage protein gene promoter-GUS fusions by exogenously applied methionine in transgenic Arabidopsis thaliana." Plant Cell Physiol.35. 927-934 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] S.Naito et al.: "Maternal effects of mto 1 mutation,that causes overaccumulation of soluble methionine,on the expression of a soybean β-conglycinin gene promoter-GUS fusion in transgenic Arabidopsis thaliana." Plant Cell Physiol.35. 1057-1063 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] K.Inaba et al.: "Isolation of an Arabidopsis thaliana mutant,mto1,that overaccumulates soluble methionine.Temporal and spatial patterns of soluble methionine accumulation." Plant Physiol.104. 881-887 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Y.Ishiwatari et al.: "Thioredoxin h is one of the major proteins in rice phloem sap." Planta. 195. 456-463 (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] S.Nakamura et al.: "Detection and characterization of Ca-dependent protein kinase in rice phloem sap." Plant Cell Physiology. 36. 19-27 (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] 松本英明: "低pH土壌と植物 植物におけるAl耐性の生理生化学" 日本土壌肥料学会編 博友社, 236 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] S.Nakamura: "Protein phosphorylation in the sieve tubes of rice plants." Plant Cell Physiol.34. 927-933 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Y.Yamamoto: "Quantitative estmation of aluminium toxicity in cultured tobacco cells:correlation between aluminium uptake and growth inhibition." Plant Cell Physiol.(in press). (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] R.Saraswati: "Identification of Sesbania species from electrophoretic patterns of seed proteins." Trop.Agric.(Trinidad). 70. 282-285 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] T.Tadano: "Secretion of acid phosphatase by the roots of crop plants under phosphorus-deficient conditions and some properties of the enzyme secreted by lupin roots." Plant nutrition-from genetic engineering to field practice,N.J.Barrow(Ed.). 99-102 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] K.Inaba: "Isolation of Arbidopsis thaliana mutations,mto,that overaccumulate soluble methionine." Plant Physiology. (in press). (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] H.Obata: "Production of SH compounds in higher plants of different tolerance Cd." Plant nutrition-from genetic engineering to field practice,N.J.Barrow(Ed.). 791-794 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 松本英明: "植物におけるAl耐性の生理生化学" 日本土壌肥料学会編.博友社(印刷中),

    • Related Report
      1993 Annual Research Report

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Published: 1993-03-31   Modified: 2016-04-21  

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