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Establishment of Molecular-Biological Basis on the Mechanisms of Aluminum Tolerance in Plants.

Research Project

Project/Area Number 05304015
Research Category

Grant-in-Aid for Co-operative Research (A)

Allocation TypeSingle-year Grants
Research Field Plant nutrition/Soil science
Research InstitutionHokkaido University

Principal Investigator

TADANO Toshiki  Faculty of Agriculture, Hokkaido University, Professor, 農学部, 教授 (40001440)

Co-Investigator(Kenkyū-buntansha) HARA Tetsuo  Faculty of Agriculture, Gifu University, Professor, 農学部, 教授 (50021720)
KONISHI Shigeki  Faculty of Agriculture, Shizuoka University, Professor, 農学部, 教授 (70026417)
WAGATSUMA Tadao  Faculty of Agriculture, Yamagata University, Professor, 農学部, 教授 (70007079)
OJIMA Kunihiko  Faculty of Agriculture, Tohoku University, Professor, 農学部, 教授 (70005669)
MATSUMOTO Tideaki  Research Institute for Bioresources, Oskayama University Professor, 資源生物科学研究所, 教授 (80026418)
横田 聡  東北大学, 農学部, 助手 (60220555)
Project Period (FY) 1993 – 1994
Project Status Completed (Fiscal Year 1994)
Budget Amount *help
¥13,000,000 (Direct Cost: ¥13,000,000)
Fiscal Year 1994: ¥5,400,000 (Direct Cost: ¥5,400,000)
Fiscal Year 1993: ¥7,600,000 (Direct Cost: ¥7,600,000)
KeywordsAluminum tolerance / Aluminum toxicity / lon channel / Development of aluminum-tolerant plants / Growth enhancement by aluminum / Aluminum-inductive protein / Aluminum tolerant microorganism / Stimulation of aluminum toxicity by Fe. / アルミニウム排除能 / アルミニウム集積能 / 原形質膜 / 培養細胞 / 低pH耐性 / カロース合成 / 根端細胞
Research Abstract

(1)It was shown that inward K ion channel existed in the plasmalemma of barley roots and that K absorption through this channel was inhibited irreversively byAl.
(2)The primary site that was suffered from Al toxicity was plasmalemma of root tip cells.
(3)A new method to isolate the Al-tolerant cells was developed on the basis of the difference in the minus charge of plasmalemma.
(4)Al toxicity was stimulated by the co-existence of Al and Fe in the grown media which was caused by an increased lipd-peroxidation.
(5)It was indicated that Al torerance of cultured cells was improved when P-starvation treatment was previously given. And it was assumed to be caused by the increase of ex-pression of genes related to the protecting mechanisms to the inhibition of cell divition and to the peroxidation of lipids in the cell membranes by Al.
(6)Al toleranct characters of carrot seeds, which were derived from regeneration of cultured cells selected for the Al tolerance, were inherited to the following seeds. It was thus shown that the cell-culture method to select tolerant cells to stresses was available practically for the development of varieties tolerant to acid soils.
(7)Growth and nutrient absorption of various plants well adapted to the acid soils were enhanced by Al. There were several plants in which Ca had no growth stimulating effects.
(8)Al-protein complexes were contained in the roots of tea plant when it was grown in the growth media with Al. It was assumed that the proteins were induced by Al.
(9)A microorganism with an extremely high tolerance to low pH,high Al and high Mn in the growth media was isolated from a soil and classified as Flavobacterium ST-3991. Using this microorganism, it may be possible to maintain a sustainable plant production through an increase of pH and decrease of Al concentration in the soil solutions.

Report

(3 results)
  • 1994 Annual Research Report   Final Research Report Summary
  • 1993 Annual Research Report

Research Products

(44 results)

All Other

All Publications (44 results)

  • [Publications] Sasaki,M.: "Comparison of the Early Response to Aluminum Stress between Tolerant and Sensitive Wheat Cultivars:Root Growth,Aluminum Content and Efflux of K^+." J.Plant Nutrition.17. 1275-1288 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Sasaki,M.: "Putative Ca^<2+> Channels of Plasma Membrane Vesicles are not Involved in the Tolerance Mechanism of Aluminum in Aluminum Tolerant Wheat (Triticum aestivum L.)Cultivar." Soil Sci.Plant Nutr.40. 709-714 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Yamamoto,Y.: "Quantitive Estimation of Aluminium Toxicity in Cultured Tobacco Cells:Correlation between Aluminium Uptake and Growth Inhibition." Plant Cell Physiol.35. 575-583 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Ezaki,B.: "Cloning and Sequencing of the cDNA Induced by Aluminum Treatment and Pi Starvation in Cultured Tobacco Cells." Physiol.Plant.93. 11-18 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Ono,K.: "Synergistic Inhibition of Growth by Aluminum and Iron of Tobacco(Nicotiana tabacum L.)Cells in Suspension Culture." Plant Cell Physiol.36(in press). (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Wagatsuma,T.: "Plasma Membrane of Younger and Outer Cells is the Primary Specific Site for Aluminum Toxicity in Roots." Plant and Soil,. (in press). (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 但野 利秋: "低pH土壌と植物、VII植物における低リン耐性の生理的機構" 博友社, 236 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Sasaki, M.: "Comparison of the Early Response to Aluminum Stress between Tolerant and Sensitive Wheat Cultivars" Root Growth, Aluminum Content and Efflux of K^+.J.Plant Nutrition. 1275-1288 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Sasaki, M.: "Putative Ca^<2+> Channels of Plasma Membrane Vesicles are not lnvolved in the Tolerance Mechanism of Aluminum in Aluminum Tolerant Wheat(Triticum aestivum L.)Cultivar" Soil Sci.Plant Nutr. 40. 709-714 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Yamamoto, Y.: "Quantitive Estimation of Aluminium Toxicity in Cultured Tobacco Cells : Correlation between Aluminium Uptake and Growth Inhibition" Plant Cell Physiol.35. 575-583 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Ezaki.B.: "Cloning and Sequencing of the cDNA lnduced by Aluminum Treatment and Pi Starvation in Cultured Tobacco Cells" Physiol.Plamt.93. 11-18 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Ono.K.: "Synergistic lnhibition of Growth by Aluminum and lron of Tobacco(Nicotiana tabacum L.)Cells in Suspension Culture" Plant Cell Physiol. 36(in press). (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Wadatsuma, T.: "Plasma Membrane of Younger and Outer Cells is the Primary Specific Site for Aluminum Toxicity in Roots" Plant and Soil. 306(in press). (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Tsuji, M.: "Stimulatory Effects of Aluminum on the Growth of Cultured Roots of Tea" Soil Sci.Plant Nutr.40. 471-476 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Konishi, M.: "Isolation and Characteristics of Acid-and Aluminum-Tolerant Bacterium" Biosci.Biotech.Biochem.58. 1960-1963 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Inaba, K.: "Isolation of an Arabidopsis thaliana Mutant, mto 1, That Cver-accumulate Soluble Methionine" Plant Physiol.104. 881-887 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Hirai, M.Y.: "Differential Regulation of Soybean Seed Storage Protein Gene Promoter-GUS Fusions by Exogeneously Applied Methionine in Transgenic Arabdopsis thaliana" Plant Cell Physiol.35. 927-934 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Naito, S.: "Maternal Effects of mto 1 Mutation, That Courses Overaccumulation of Soluble Methionine, on the Expression of a Soybean beta-Conglysinin Gene Promoter-GUS Fusion Intransgenic Arabidopsis thaliana" Plant Cell Physiol.35. 1507-1063 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Tadano, T.: Low pH Soils and Plants.VII.Physiological Mechanisms of Low Phosphorus Tolerance in Plants. Hakuyu-sha(in Japanese), 236 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Sasaki,M.: "Comparison of the Early Response to Aluminum Stress between Tolerant and Sensitive Wheat Cultivars:Root Growth,Aluminum Content and Efflux of K^+." J.Plant Nutrition. 17. 1275-1288 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Sasaki,M.: "Putative Ca^<2+> Channels of Plasma Membrane Vesicles are not lnvolved in the Tolerance Mechanism of Aluminum in Aluminum Tolerant wheat(Triticum aestivum L.)Cultivar." Soil Sci.Plant Nutr.40. 709-714 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Yamamoto,Y.: "Ouantitive Eatimation of Aluminium Toxicity in Cultured Tobacco Cells:Correlation between Aluminium Uptake and Growth inhibition." Plant Cell Physiol.35. 575-583 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Ezaki,B.: "Cloning and Sequencing of the cDNA lnduced by Aluminum Treatment and Pi Starvation in Cultured Tobacco Cells." Physiol.Plant.93. 11-18 (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] Ono,K.: "Synergistic lnhibition of Growth by Aluminum and lron of Tobacco(Nicotiana tabacum L.)Cells in Suspension Culture.Plant Cell Physiol." (in press). (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] Wagatsuma,T.: "Plasma Membrane of Younger and Outer Cells is the Primary Specific Site for Aluminum Toxicity in Roots." Plant and Soil. (in press). (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] Konishi,M.: "lsolation and Characterstics of Acid-and Aluminum-Tolerant Bacterium." Biosci.Biotech.Biochem.58. 1960-1963 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Inaba,K.: "lsolation of an Arabidopsis thaliana Mutant,mto 1,That Over-accumulate Soluble Methionine." Plant Physiol.104. 881-887 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Hirai,M.Y.: "Differential Regulation of Soybean Seed Storge Protein Gene Promoter-GUS Fusions by Exogeneously Applide Methionine in Transgenic Arabdopsis thaliana." Plant Cell Physiol.35. 927-934 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Naito,S.: "Maternal Effects of mto 1 Mutation,That Courses Overaccumulation of Soluble Methionine,on the Expression of a Soybean β-Conglysinin Gene Promoter-GUS Fusion lntransgenic Arabidopsis thaliana." Plant Cell Physiol.35. 1057-1063 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 但野利秋: "低pH土壌と植物、VII.植物における低リン耐性の生理的機構.28HD01:1994" 博友社, 32

    • Related Report
      1994 Annual Research Report
  • [Publications] Ikeda,H.: "Ultrastractural changes of the root tip cells in baeley induced by a comparatively low concentration of aluminum." Soil Science and Plant Nutrition. 39. 109-117 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Tadano,T.: "Enhancement of salt tolerance in rice plant induced by aluminum in the growth medium." Proc.Third Int.Symposium“Plant-Soil Interactions at Low pH". in Press. (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] 松本英明: "アルミニウムを中心としたイオンストレスによる植物の障害と耐性機構に関する研究" 日本土壌肥料学雑誌. 64. 251-254 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Kasai,M.: "Possible involvement of abscisic acid in increase in activities of two vacuolar H-pumps in barley roots under aluminum stress." Plant Cell Physiology. 34. 1335-1338 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Yamamoto,Y.: "Aluminum tolerance caused by phosphate starvation in cultured tobacco cells." Proc.Third Int.Symposium“Plant-Soil Interactions at Low pH". (in Press). (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] Yamamoto,Y.: "Determining factors of aluminum toxicity in cultured tobacco cells:Medium components and cellular growth conditions." Proc.Third Int.Symposium“Plant-Soil Interactions at Low pH". (in Press). (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] Sasaki,M.: "Involvement of plasma membrane potential in the tolerance mechanism of plant root to aluminum toxicity." Proc.Third Int.Symposium“Plant-Soil Interactions at Low pH". (in Press). (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] Kasai,M.: "Increase of ATP-dependent H-pump activity of tonoplast of barley roots by aluminum stress:Possible involvement of abscidic acid for the regulation." Proc.Third Int.Symposium“Plant-Soil Interactions at Low pH". (in Press). (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] Yamamoto,Y.: "Quantitative estimation of aluminum toxicity in cultured tobacco cells:correlation between aluminum uptake and growth inhibition." Plant Cell Physiology. 35(in Press). (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] Hara,T.: "Growth response of three plants to different aluminum ions in culture solution." Res.Bull.Fac.Agr.Gifu Univ.58. 93-100 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Tsuji,M.: "Stimulatory effects of aluminum on the growth of cultured roots of tea." Soil Science and Plant Nutrition. 40(in Press). (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] Yokota,S.: "Physiological response of root tip of alfalfa to low pH and aluminum stress in water culture." Proc.Third Int.Symposium“Plant-Soil Interactions at Low pH". (in Press). (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] 松本英明: "低pH条件における植物と土壌の相互作用" 日本土壌肥料学会(博友社)(印刷中), (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] 我妻忠雄: "低pH条件における植物と土壌の相互作用" 日本土壌肥料学会(博友社)(印刷中), (1994)

    • Related Report
      1993 Annual Research Report

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Published: 1993-03-31   Modified: 2016-04-21  

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