| Project/Area Number |
05304027
|
|---|
| Research Category |
Grant-in-Aid for Co-operative Research (A)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Pathological medical chemistry
|
|---|
| Research Institution | KYOTO UNIVERSITY |
|---|
Principal Investigator |
ICHIKAWA Atsushi Kyoto Univ., Fac.Pharm.Sci., Prof., 薬学部, 教授 (10025695)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
NARUMIYA Shuh Kyoto Univ., Fac.Med., Prof., 医学部, 教授 (70144350)
SUDA Tatsuo Showa Univ., School of Dentistry, Prof., 歯学部, 教授 (90014034)
ISHIMURA Yuzuru Keio Univ., Fac.Med., Prof., 医学部, 教授 (40025599)
OKAMOTO Mitsuhiro Osaka Univ., Fac.Med., Prof., 医学部, 教授 (90028613)
INOUE Keizo Univ.Tokyo, Fac.Pharm.Sci., Prof., 薬学部, 教授 (30072937)
清水 孝雄 東京大学, 医学部, 教授 (80127092)
|
|---|
| Project Period (FY) |
1993 – 1995
|
| Project Status |
Completed (Fiscal Year 1995)
|
| Budget Amount *help |
¥31,200,000 (Direct Cost: ¥31,200,000)
Fiscal Year 1995: ¥10,600,000 (Direct Cost: ¥10,600,000)
Fiscal Year 1994: ¥9,700,000 (Direct Cost: ¥9,700,000)
Fiscal Year 1993: ¥10,900,000 (Direct Cost: ¥10,900,000)
|
|---|
| Keywords | PAF acetylhydrolase / P450 (11beta, aldo) / cyclooxygenase / aldosterone synthetase / PGE2 receptor subtypes / 1alpha, 25-dihydroxyvitamin D3-24-hydroxylase / midkine / PAF receptor / P450(11β,aldo) / シクロオキシゲナーゼ-2(COX-2) / コルチコステロン合成酵素 / PGE_2受容体サブタイプ / 1α,25-(OH)_2ビタミンD-24水酸化酵素 / PAF受容体遺伝子 / アラキドン酸 / プロスタグランジン / PAF / ステロイド産生 / レチノイン酸 / ホスホリパーゼA_2 / リポキシゲナーゼ |
|---|
| Research Abstract |
The project has intended to research in molecular structures and functions of rate-limiting enzymes in the synthesis, and receptors/signal transduction systems in the biological activities for various bioactive lipid modulators ; prostaglandins, PAF,steroid hormones, and vitamin A and D.Summary obtained by research groups as below ;
(1) Prostaglandins and PAF group ; cDNA cloning and its expression & purification of 12-lipoxygenase, Induction of prostaglandin H synthase-2 and 12-lipoxygenase by cytokinines or prostagalndins (by Yamamoto) . cDNA cloning and expression of PAF acetylhydrolase, and identification of its alpha subunit of enzyme encoding Miller-Dieker lissencephaly (by Inoue) . cDNA cloning and tissue distribution, and its gene expression and signal transduction of PAF (by Shimizu) , and of 5 basic prostaglandin receptor types (DP,EP,FP,IP,TP) , 4EP subtypes and 3 EPs isoforms (by Narumiya & Ichikawa) . (2) Steroid hormone group ; Regulation of steroid 11beta/18-hydroxylation and aldosterone production, and Isolation and characterization of three CYP11B genes, comprising 11beta-hydroxylase, aldosterone synthetase and a novel genes, analysis of promoter regions of CYP11B genes (by Ishimura & Okamoto) . Expression and tissue specific localization of Ad4BP,a steroidgenic cell-specific transcription factor (by Morohashi) . (3) Vitamins A & D group ; Isolation and characterization, expression and tissue distribution of retinoic acid responsive gene product, midkine, growth/differentiation factor of various tissues (by Muramatsu) . Regulation of mRNA expression, and Identification of vitamin D responsive element in the 5'-flanking region of 1alpha, 25-dihydroxyvitamin D3-24-hydroxylase (by Suda)
|
