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Molecular Biology of the Kidney-Molecular Analysis of Structure and Function Relationship

Research Project

Project/Area Number 05304037
Research Category

Grant-in-Aid for Co-operative Research (A)

Allocation TypeSingle-year Grants
Research Field Kidney internal medicine
Research InstitutionTokyo Medical and Dental University

Principal Investigator

MARUMO Fumiaki  Tokyo Medical & Dental Univ.Internal Medicine.Professor, 医学部, 教授 (00050443)

Co-Investigator(Kenkyū-buntansha) SAKAI Osamu  Tokyo Jikei Medical University.Internal Medicine.Professor, 医学部, 教授 (40056560)
KOIDE Hikaru  Jyuntendo University.Internal Medicine.Professor, 医学部, 教授 (80052968)
KUROKAWA Kiyoshi  Tokyo University.Internal Medicine.Professor, 医学部, 教授 (30167390)
OKUDA Seiya  Kyushuu University.Internal Medicine.Assistant Professor, 医学部, 講師 (80158823)
ARAKAWA Masaaki  Niigata University.Internal Medicine.Professor, 医学部, 教授 (80069012)
Project Period (FY) 1993 – 1994
Project Status Completed (Fiscal Year 1994)
Budget Amount *help
¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 1994: ¥2,400,000 (Direct Cost: ¥2,400,000)
KeywordsMolecular biology / Nephrology / Cloning / Glomerulus / Nephron / Transporter / Basement Membrane / Hormone / cloning / Transporter / Basement membrane
Research Abstract

We have investigated three major aspects of the kidney.
1) Glomerular matrix and glomerular function. We found a mutations of alpha 5 chain of typelV collagen gene in patients with Alport syndrome. This result confirm the significance of basement membrane collagen in glomerular function. We also showed that glycosilated substances stimulates the transcription of extracellular matrix genes. This stimulation is important for the sclorotic changes of glomerulus frequently observed in diabetes meditus.
2) Regulation of bioactive substances and their receptors. We showed the presence of TGFb mRNA in glomerulus, all nephron segments, small renal arteiries. On the other hand, mRNA of LTBP,which is important for activation of TGFb, is present only in glomerulus and small renal arteiries. We also developed a new acurate method for quantification of RTPCR which is critically needed for small piece of tissues such as dissected nephron segments. Usin this method we showed the nephron localization of mRNA of PGE2 receptor.
3) Cloning and characterization of kidney trnasport proteins. We cloned a kidney collecting duct water channel named AQP3, which is localized at the basolateral membrane. We showed many regulational mechanisms of AQP2 at trnascriptional, protein, and cellular levels. We anso cloned a rat dipeptide transporter in the kidney, and found that this transporter works as a rout for cepharosporin antibiotics.

Report

(3 results)
  • 1994 Annual Research Report   Final Research Report Summary
  • 1993 Annual Research Report
  • Research Products

    (19 results)

All Other

All Publications (19 results)

  • [Publications] Masanori Kitamura: "Heparin selectively inhibits gene expression of matrix metalloproteinase transin in cultured mesangial cells." Biochem,Biophys,Res,Commun.203. 1333-1338 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kiyoshi Takami: "TGF-β1 in glomerulosclerosis and interstitial Fibrosis of adriamycin nephropathy." Kidney Int.45. 525-536 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Akihiko Saito: "A deletion mutation in the 3'end of the α5 collagen gene in juvanile-onset Alport Syndrome." J.Amer.Soc,Nephrol.4. 1649-1653 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Makoto Tanaka: "Regulation of apolipoprotein B production and secretion in response to the change of intracellular cholesteryl ester contents in rabbit hepatocyte." J.Biol.Chem.268. 12713-12718 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Shigeo Taniguchi: "Detection and quantitation of EP_3 prostaglandin E_2 receptor mRNA along mouse nephson segments by RT-PCR." Am.J,Physiol,. 266. C1453-C1458 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Matsuhiko Hayashi: "Expression and distribution of aquaporin of collecting duct are regulated by vasopressin V_2 receptor in rat kidney." J,Clin.Invest. 94. 1778-1783 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Masanori Kitamura: "Heparin selectirely inhibits gene expression of matrix metalloproteinase transin in cultured mesangial cells." Biochem.Biophys.Res.Commun.203. 1333-1338 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Kiyoshi Takami: "TGF-β1 in glomerulosclerosis and interstitial fibrosis of adriamycin nephropathy." Kidney Int.45. 525-536 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Akihiko Saito: "A deletion mutation in the 3'end of the α5 collagen gene in jurenile-onset Alport syndrome." J.Amer.Soc.Nephrol.4. 1649-1653 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Makoto Tanaka: "Regulation of apolipoprotein B production and secretion in response to the change of intracellular cholesteryl ester contents in rabbit hepatocyte." J.Biol.Chem.268. 12713-12718 (1993)

    • Related Report
      1994 Annual Research Report
  • [Publications] Shigeo Taniguchi: "Detection and quantitation of EP_3 prostaglandin E_2 recepfor mRNA along mouse nephron segments by RT-PCR." Am.J.Physiol.266. C1453-C1458 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Matsuhiko Hayashi: "Expression and distribution of aquaporin of collecting duct are regulated by vasopressin V_2 receptor in rat kidney." J.Clin.Invest. 94. 1778-1783 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] OWADA,A.,et.al.: "Decreased Lithium Clearance in Patiects with Hyperthyriodism." Nephron. 64. 37-41 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] KOIKE,J.,et.al.: "Effect of Urodilatin on cGMP Accumulation in the Kidney." J Am Soc Nephr. 3. 1705-1709 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] UCHIDA,S.,et.al.: "Molecular Cloning of a Chloride Channel That Is Regulated by Dehydration and Expressed Predominantly in Kidney Medulla." J Biolo Chem. 268. 3821-3824 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] SASAKI,S.,et.al.: "Cloning and expression of two renal transport proteins which are involved in urinary concentration mechanisms:collecting duct water channel and thin ascending limb chloride channel." John Libbey Eurotext. 325-335 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] YANG,T.,et.al.: "Effect of hyperosmolality on production and mRNA expression of Et-1 in inner medullary collecting duct." Am Phys Soc. F684-F689 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] TOMITA,K.,et.al.: "Effects of Et-1 on water and collecting ducts of the rat." Am Phys Soc. F690-F696 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] FUSHIMI,K.,et.al.: "Cloning and expression of apical membrance water channel of rat kidney collecting tubule." Nature. 361. 549-552 (1993)

    • Related Report
      1993 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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