• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Basic study of optic nerve regeneration by transplantation for clinical application

Research Project

Project/Area Number 05304040
Research Category

Grant-in-Aid for Co-operative Research (A)

Allocation TypeSingle-year Grants
Research Field Ophthalmology
Research InstitutionOsaka University

Principal Investigator

FUKUDA Yutaka  Osaka University, Med.Sch.Prof., 医学部, 教授 (90028598)

Co-Investigator(Kenkyū-buntansha) HORIE Hidenori  Yokohama City Univ.Med.Sch.Lec., 医学部, 講師 (80046135)
TAKAHASHI Hiroshi  Univ.Occu.Env.Health, Assoc.Prof., 助教授 (00129511)
WAKAKURA Masato  Kitasato Univ.Med.Sch.Assoc.Prof., 医学部, 助教授 (50137931)
SAITO Takehiko  Univ. of Tsukuba Biol.Sci.Prof., 生物科学系, 教授 (20081630)
TAMAI Makoto  Tohoku Univ.Med Sch.Prof., 医学部, 教授 (90004720)
Project Period (FY) 1993 – 1994
Project Status Completed (Fiscal Year 1994)
Budget Amount *help
¥14,000,000 (Direct Cost: ¥14,000,000)
Fiscal Year 1994: ¥6,500,000 (Direct Cost: ¥6,500,000)
Fiscal Year 1993: ¥7,500,000 (Direct Cost: ¥7,500,000)
KeywordsRetina / Optic nerve / Transplantation / Regeneration / Cell differentiation / Amino acid receptor / Retinal pigment epithelium / Trophic factor / 機能回復 / 組織構築 / 神経栄養因子
Research Abstract

1. New observations in non-mammalian retina
Saito revealed the sequence of apperance of retinal neurons during retinal regeneration from the pibment epithelium in the newt by histochemical and electrophysiological studies. Araki suggested a genetic program in the transdifferentiation of retinal neurons from pigment epithelial cells based on observations in a quail mutant.
2. Trophic factors for the promotion of retinal regeneration
Horie cultured retinal explants of new-born mice and adult rats in collagen gel and revealed that hepatocyte conditioned media enhanced process formation from the retinal tissue. Wakakura studied intracellular Ca^<2+> responses to glutamate in rabbit Muller cells and suggested a protective role of Muller cells against retinal injury.
3. Axonal regeneration of retinal ganglion cells after peripheral nerve transplantation
Watanabe revealed that alpha-type ganglion cells of the cat retina have the highest ability in axonal regeneration after peripheral nerve graft. Tauchi showed gap junctions in degenerating or regenerating ganglion cells of the rat retina. Fukuda characterized the receptive field properties of regenerated ganglion cells of the cat retina by recording single unit activities from transplanted nerve graft. Sasaki demonstrated that visual discrimination could be retrieved by transplantation in hamsters with damaged visual pathway.
4. Regeneration of human retina
Tamai developed an animal model of damaged retinal pigment epithelium in which effective surgical procedures were tested to restore the damaged retinal tissue. Takahashi succeeded in culturing human retinal ganglion cells in the presence of Muller cells.

Report

(3 results)
  • 1994 Annual Research Report   Final Research Report Summary
  • 1993 Annual Research Report
  • Research Products

    (67 results)

All Other

All Publications (67 results)

  • [Publications] Fukuda Y,et al: "Axonal regeneration of cat's retinal ganglion cells by peripheral nerve transplant." Biomedical Research 15,Supplement22GD01:1. 123-125 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Yamashita M,Fukuda Y,et al: "Muscarinic acetylcholine responses in the early embryonic chick retina." Journal of Neurobiology. 25. 1144-1153 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kosaka J,Fukuda Y,et al: "Expression and localization of γ-aminobutyric acid A(GABA_A)recetor α1 subunit and L-glutamate decarboxylase(GAD)mRNAs in rat retina:an analysis by in situ hybridization." Molecular Brain Research. 25. 163-167 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kimizuka Y,Tamai M,et al: "Repair of damaged retinal pigment epithelial cells in the rabbit eye and effects of b-FGF." Investigative Opthalmology & Visual Science. 35(4). 1768- (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Shoji A,Tamai M,et al: "Abnormal localization of immunoreactive neurotransmitters in the experimental retinal degeneration." Investigative Opthalmology & Visual Science. 35(4). 1956- (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 玉井 信: "網膜色素上皮移植を目指して." 眼科手術. 7. 439-441 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Wakakura M,et al: "Cytosolic calcium transient increase through the AMPA/Kainate receptor in cultured Muller cells." Vision Research. 34. 1105-1109 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Horie H,et al: "Regional differences in neurite regeneration of postnatal retinal explants." Brain Research Bulletin. 34. 381-384 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Takano M & Horie H: "Critical period for degradation of adult rat retinal ganglion cells and their regeneration capability after axotomy." Neuroscience Letters. 175. 129-132 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Horie H,et al: "Three-demensional cell aggregation enhances growth-promoting activity of NGF in adult DRG." NeuroReport. 6. 37-41 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Saito T,et al: "Study of the regenerating newt retina by electrophysiology and immunohistochemistry(Bipolar-and cone-specific antigen localization)." Journal of Experimental Zoology. 270. 491-500 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Sakai H & Saito T: "Development of voltage-dependent inward current in dissociated newt retinal pigmentepithelial cells in culture." NeuroReport. 5. 933-936 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 徳永 史生,斉藤 建彦: "視覚器-視興奮の分子機構と網膜再生." 生体の科学. 45. 62-70 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Araki M,et al: "Developing rat pineal manifest potential of neuronal differentiation in vitro." Neuroscience Research. 20. 57-69 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 野中 徹郎,荒木 正介,ほか: "哺乳類松果体の移植." 脳と神経. 46. 315-324 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 荒木 正介: "神経の発生における分化の多能性とその制御-松果体の培養モデル." 京都府立医大雑誌. 104. 233-247 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 田内 雅規,ほか: "視神経切断による網膜神経節細胞の変性." 神経眼科. 11. 263-270 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Watanabe M,Fukuda Y,et al: "Myelination of regenerated optic nerve fibers in peripheral nerve graft of adult cats." Experimental Brain Research. 98. 39-43 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 渡部 眞三: "哺乳動物中枢神経系の軸索再生-視神経再生の研究を中心に." 蛋白質.核酸・酵素. 40. 136-142 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Watanabe M,Fukuda Y,et al: "Number and dendritic morphology of retinal ganglion cells that survived after axotomy in adult cats." Journal of Neurobiology. 27(2)(in press). (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Watanabe, M., Sawai, H., Fukuda, Y.: "Number, distribution, and morphology of retinal ganglion cells with axons regenerated into peripheral nerve graft in adult cats" Journal of Neuroscience. 13. 2105-2117 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Sasaki, H., Inoue, T., Iso, H., Fukuda, Y., Hayashi, Y.: "Light-dark discrimination after sciatic nerve transplantationto the sectioned optic nerve in adult hamsters" Vision Research. 33. 877-880 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Fukuda, Y., Miyoshi, T., Sugioka, M., Sawai, H., Watanabe, M.: "Axonal regeneraiton of cat's retinal ganglion cells by peripheral nerve transplant" Biomedical Research. 15. 123-125 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Horie, H., Ito, S., Takano, M.: "Regional differences in neurite regeneration of postnatal mouse retinal explants" Brain Research Bulletin. 34. 381-384 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Wakakura, M., Yamamoto, N.: "Cytosolic calcium transient increase through the AMPA/kainate receptor in cultured Muller cells" Vision Research. 34. 1105-1109 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Araki, M., Nonaka, T., Akagawa, K., Kimura, H., Mashiko, T.: "Developing rat pineal cells manifest potential of neuronal differentiation in vitro" Neuroscience Research. 20. 57-69 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Watanabe, M., Sawai, H., Fukuda, Y.: "Myelination of regenerated optic fibers in peripheral nerve graft of adult cats" Exp.Brain Research. 98. 39-43 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Saito, T., Kaneko, Y., Maruo, F., Niino, M., Sakaki, Y.: "Study of the regenerating newt retina by electrophysiology and immunohistochemistry" Journal of Exp.Zoology. 270. 491-500 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Fukuda Y,et al: "Axonal regeneration of cat's retinal ganglion cells by peripheral nerve transplant." Biomedical Research 15,Supplement. 1. 123-125 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Yamashita M, Fukuda Y,et al: "Muscarinic acetylcholine responses in the early embryonic chick retina." Journal of Neurobiolgy. 25. 1144-1153 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Kosaka J,Fukuda Y,et al: "Expression and localization of γ-aminobutyric acid A(GABA_A)recetor α1 subunit and L-glutamate decarboxylase (GAD) mRNAs in rat retina:an analysis by in situ hybridization." Molecular Brain Research. 25. 163-167 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Kimizuka Y, Tamai M,et al: "Repair of damaged retinal pigment epithelial cells in the rabbit eye and effects of b-FGF." Investigative Opthalmology & Visual Science. 35(4). 1768- (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Shoji A,Tamai M,et al: "Abnormal localization of immunoreactive neurotransmitters in the experimental retinal degeneration." Investigative Opthalmology & Visual Science. 35(4). 1956- (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 玉井 信: "網膜色素上皮移植を目指して." 眼科手術. 7. 439-441 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Wakakura M,et al: "Cytosolic calcium transient increase through the AMPA/Kainate receptor in cultured Muller cells." Vision Research. 34. 1105-1109 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Horie H,et al: "Regional differences in neuriteregeneration of postnatal retinal explants." Brain Research Bulletin. 34. 381-384 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Takano M & Horie H: "Critical period for degradation of adult rat retinal ganglion cells and their regeneration capability after axotomy." Neuroscience Letters. 175. 129-132 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Horie H,et al: "Three-demensional cell aggregation enhances growth-promoting activity of NGF in adult DRG." NeuroReport. 6. 37-41 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Saito T,et al: "Study of the regenerating newt retina by electrophysiology and immunohistochemistry(Bipolar-and cone-specific antigen localization)." Journal of Experimental Zoology22GD11:270. 491-500 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Sakai H & Saito T: "Development of voltage-dependent inward current in dissociated newt retinal pigmentepithelial cells in culture." NeuroReport. 5. 933-936 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 徳永 忠生,斉藤 建彦: "視覚器-視興奮の分子機構と網膜再生." 生体の科学. 45. 62-70 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Araki M,et al: "Developing rat pineal manifest potential of neuronal differentiation in vitro." Neuroscience Research. 20. 57-69 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 野中 徹郎,荒木 正介,ほか: "哺乳類松果体の移植." 脳と神経. 46. 315-324 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 荒木 正介: "神経の発生における分化の多能性とその制御-松果体の培養モデル.21GC16:京都府立医大雑誌" 104. 233-247 (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] 田内 雅規,ほか: "視神経切断による網膜神経節細胞の変性." 神経眼科. 11. 263-270 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Watanabe M,Fukuda Y,et al: "Myelination of regenerated optic nerve fibers in peripheral nerve graft of adult cats." Experimental Brain Research. 98. 39-43 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 渡部 眞三: "哺乳類動物中枢神経系の軸索再生-視神経再生の研究を中心に." 蛋白質.核酸・酵素. 40. 136-142 (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] Watanabe M,Fukuda Y,et al: "Number and dendritic morphology of retinal ganglion cells that survived after axotomy in adult cats." Journal of Neurobiology. 27(2)(in press). (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] Watanabe M,Fukuda Y,et al: "Number,distribution and morphology of retinal ganglion cells that regenerated into peripheral nerve graft in adult cats." J.Neurosci.13. 2105-2117 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Yamashita M,Fukuda Y: "Incurvation of early embryonic neural retina by acetylcholine through muscarinic receptors." Neurosci.Lett.163. 215-218 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Sasaki H,Fukuda Y,et al: "Early and late-induced field responses correspond to ON and OFF receptive field components in hamster superior colliculus." Prog.Brain Res.95. 103-110 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Yamashita M,Fukuda Y: "Calcium channels and GABA receptors in the early embryonic chick retina." J.Neurobiol.24. 1600-1614 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Sasaki H,Fukuda Y,et al: "Light-dark discrimination after sciatic nerve transplantation to the sectioned optic nerve in adult hamsters." Vision Res.33. 877-880 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Wakakura M,Foulds WS: "Heat shock response and thermal resistance in cultured human retinal pigment epithelium." Exp.Eye Res.56. 17-24 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 若倉雅登: "視神経疾患の治療." あたらしい眼科. 10. 1073-1081 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Wakakura M: "Retina and optic nerve disorders." Curr.Opin.Opthalmol.4. 16-21 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Araki M,Orii H,et al: "Retinal differentiation from multipotential pineal cells of the embryonic quail." Neurosci.Res.18. 63-72 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 日高聡,田内雅規: "軸索切断後、長期生存した網膜神経節細胞に観察されたギャップ結合." 神経組織の成長・再生・移植. 5. 51-52 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 田内雅規: "軸索を切断したラット網膜神経節細胞の三次元形態について." 第17回日本神経科学大会抄録集. 106 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Chi H,Horie H,et al: "Isolation and age-related characterization of mouse Schwann cells from dorsal root ganglion explants in type I collagen gels." J.Neurosci.Res.35. 183-187 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Sango K,Horie H,et al: "Age-related changes of DRG neuronal attachment to extracellular matrix proteins in vitro." Neuroreport. 4. 663-666 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Hikawa N,Horie H,et al: "Macrophage-enhanced neurite regeneration of adult dorsal root ganglia neurons in culture." Neuroreport. 5. 41-44 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Kaneko Y,Saito T,et al: "Development of voltage gated currents in newt retinal neuroblasts in culture." Neuroreport. 4. 775-778 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 徳永史生,斉藤建彦: "視覚器-視興奮の分子機構と網膜再生." 生体の科学. 45. 62-70 (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] Ogawa M,Araki M,et al: "Early changes of macrophage-like immunoreactivity in the rat inferior olive after intraperitoneal 3-acetylpyridine injection." Brain Res.610. 135-140 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Kawakami K,Araki M,et al: "Spl binds to the AMOG(Adhesion Molecule on Glia)regulatory element that functions as a positive transcription regulatory element in astrocytes." J.Neurosci.Res.35. 138-146 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Nonaka T,Araki M,et al: "The capacity of central and peripheral catecholaminergic neurons to innervate the pineal and cerebral cortex of the rat:in vitro immunohistochemical observations." Cell Tissue Res.273. 525-531 (1993)

    • Related Report
      1993 Annual Research Report

URL: 

Published: 1993-04-01   Modified: 2017-10-06  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi