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Study of the mechanism of bone formation using morphological and functional analyzes

Research Project

Project/Area Number 05304042
Research Category

Grant-in-Aid for Co-operative Research (A)

Allocation TypeSingle-year Grants
Research Field Morphological basic dentistry
Research InstitutionShowa University

Principal Investigator

YOSHIKI Shusaku  Showa University, School of Dentistry, Professor, 歯学部, 教授 (30085740)

Co-Investigator(Kenkyū-buntansha) OZAWA Hidehiro  Niigata Univ., School of dentistry, Professor, 歯学部, 教授 (60018413)
KUMEGAWA Masayoshi  Meikai Univ., School of Dentistry, Professor, 歯学部, 教授 (40049367)
SUDA Tatsuo  Showa Univ., School of Dentistry, Professor, 歯学部, 教授 (90014034)
SUZUKI Fujio  Osaka Univ., School of Dentistry, Professor, 歯学部, 教授 (40028717)
SASAKI Satoshi  Tokyo Medical and Dental Univ., School of Dentistry, Professor, 歯学部, 教授 (80013803)
Project Period (FY) 1993 – 1994
Project Status Completed (Fiscal Year 1994)
Budget Amount *help
¥11,500,000 (Direct Cost: ¥11,500,000)
Fiscal Year 1994: ¥5,300,000 (Direct Cost: ¥5,300,000)
Fiscal Year 1993: ¥6,200,000 (Direct Cost: ¥6,200,000)
Keywordsbone formation / osteoblast / chondrocyte / osteoclast / osteocyte / BMP / in situ hybridization / cell culture / in situ hybridization / In situ hybridization
Research Abstract

The mechanism involved in bone formation has been investigated using morphological, biochemical and molecular biological techniques, and the following results are obtained.
1.The important roles of BMP-2 in the differentiation process of osteoblasts has been demonstrated using various kinds of cell lines (Yoshiki S., et al.).
2.The age-related decreases in mRNA expression for bone matrix proteins in osteoblasts were revealed by in situ hybridization(Yoshiki S., et al.).
3.Several genes for BMPs and BMP receptors were cloned. The changes in mRNA expression for Hox genes were analyzed during the process of ectopic bone formation induced by BMP(Sasaki S., et al.).
4.BMP-2 inhibited myogenic differentiation in myoblasts, and converted their differentiation pathway into osteoblast cell lineage (Suda T., et al.).
5.The tissue distribution of ChM-1 was revealed by immunohistochemistry and in situ hybridization. The important roles of ChM-2 was demonstrated in the process of bone formation and bone resorption(Suzuki F., et al.).
6.The new technique for isolation of osteocytes was developed (Kumegawa M., et al.).
7.Interaction among osteoblasts, osteocytes and osteoclasts were investigated using ultrastructural histochemistry and radar microscope(Ozawa H., et al).

Report

(3 results)
  • 1994 Annual Research Report   Final Research Report Summary
  • 1993 Annual Research Report
  • Research Products

    (14 results)

All Other

All Publications (14 results)

  • [Publications] Ikeda T.et al.: "Age-related reduction in bone matrlx protein mRNA expression in rat bone tlssues:appllcation of histomorphometry to in situ hybridization." Bone. 16. 17-23 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Katagiri T.et al.: "Bone morphogenetic protein-2 converts the differentlation pathway of C2C12 myoblasts into the osteoblast lineage.21GC02:J.Cell Biol." 127. 1755-1766 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 鈴木不二男: "軟骨由来コンドロモジュリン・Iのアンジオインヒビン作用" 実験医学. 12. 1004-1011 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Iimura T.et al.: "Changes in momeobox-containing gene expression during ectopic bone formation induced by bone morphogenetic protein." Biochem.Boiophys.Res.Commun.201. 980-987 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kamioka H.et al.: "Divalent cations elevate cytosolic calcium of chick osteocytes." Biochem.Biophys.Res.Commun.204. 519-524 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Ikeda T., et al :"Age-related reduction in bone matrix mRNA expression in rat bone tissues : application of histomorphometry to in situ hybridization." Bone. 16. 17-23 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Katagiri T., et al :"Bone morphogenetic priotein-2 converts the differentiation pathway of C2C12 myoblasts into the osteoblast lineage." J.Cell Biol.127. 1755-1766 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Iimura T., et al. :"Changes in homeobox-containing gene expression during ectopic bone formation induced by bone morphogenetic protein." Biochem.Biophys.Res.Commun.201. 980-987 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kamika H., et al. :"Divalent cations elevate cytosolic calcium of chick osteocytes." Biochem.Biophys.Res.Commun.204. 519-524 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Ikeda T,et.al.: "Age-related reduction in bone matrix protein mRNA expression in rat bone tissues:application of histomorphometry to in situ hybridization." Bone. 16. 17-23 (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] Katagiri T, et al.: "Bone morphogenetic protein-2 converts the differentiation pathway of C2C12 myoblasts into the osteoblast lineage." J.Cell.Biol.127. 1755-1766 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 鈴木不二雄: "軟骨由来コンドロモジュリン-Iのアンジオインヒビン作用" 実験医学. 12. 1004-1011 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Iimura T, et al.: "Changes in momeobox-containing gene expression during ectopic bone formation induced by bone morphogenetic protein." Biochem.Boiophys.Res.Commun.201. 980-987 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Kamioka H, et al.: "Divalent cations elevate cytosolic calcium of chick osteocytes." Biochem.Biophys.Res.Commun.204. 519-524 (1994)

    • Related Report
      1994 Annual Research Report

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Published: 1993-04-01   Modified: 2016-04-21  

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