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Studies on the microbiological basic technologies for bioremediation

Research Project

Project/Area Number 05304051
Research Category

Grant-in-Aid for Co-operative Research (A)

Allocation TypeSingle-year Grants
Research Field 環境保全
Research InstitutionThe University of Tokyo

Principal Investigator

KODAMA Tohru  Univ.of Tokyo, Dept.of Biotechnol., Professor, 農学部, 教授 (30011901)

Co-Investigator(Kenkyū-buntansha) YAGI Osami  Nat.Inst.Environ., Div.of Water and Soil Env., Director, 水土壌圏環境部, 室長研究職 (40132865)
OHTAKE Hisao  Hirosima Univ., Dept.of Ferment.Technol., Professor, 工学部, 教授 (10127483)
FURUKAWA Kensuke  Kyushu Univ., Dept.OF Ferm., Professor, 農学部, 教授 (90221556)
KATO Nobuo  Kyoto Univ., Dept.of Agric.Chem., Professor, 農学部, 教授 (50026556)
IIJIMA Shinji  Nagoya Univ., Dept, of Biol.Func., Professor, 工学部, 教授 (00168056)
高木 正道  東京大学, 農学部, 教授 (50018339)
Project Period (FY) 1993 – 1994
Project Status Completed (Fiscal Year 1994)
Budget Amount *help
¥7,900,000 (Direct Cost: ¥7,900,000)
Fiscal Year 1994: ¥4,100,000 (Direct Cost: ¥4,100,000)
Fiscal Year 1993: ¥3,800,000 (Direct Cost: ¥3,800,000)
KeywordsBioremediation / Genetical breeding / Bioreclamation of soils / Trichloroethylene / PCB degradation / Reduction of heavy metals / Denitrification / Degradation enzyme genes / 遺伝子のクローニング / 脱窒関連酵素
Research Abstract

Through our research works performed during last year, the concept for "Bioremediation" was made clear, and the present state of the worldwide research development was fully understood. On the basis of these results, the microbiological research subjects for the practical bioremediation were investigated and discussed. Some of our members participated and presented the papers in the "OECD Workshop on Bioremediation Tokyo'94", held in November 1994. The effectiveness and safety as well as the necessity of international cooperation for the application of biotechnology for encironmental crises were discussed and confirmed at the workshop. Fundamental researches for the microbial degradation of BHC,chlorinated aromatic hydrocarbons, TCE,PEG and other recalcitrant compounds were developed. The degrading enzyme genes were analyzed and further investigated for enhancing the gene functions for the purpose of their practical use for bioremediation. Members belonging to private companies exploited the supporting technologies to establish the practical bioremediation, for example, improvement of the apparatus for effective treatment of polluted soil or waste water. The results of the present research works will be published by a publisher, Gihodo Shuppan Co.Ltd., in May 1995 in Japanese under the title "Various Kinds of Microorganisms Preserving the Environment".

Report

(3 results)
  • 1994 Annual Research Report   Final Research Report Summary
  • 1993 Annual Research Report
  • Research Products

    (47 results)

All Other

All Publications (47 results)

  • [Publications] Lee,J.-H.et al.: "Identification of the metabolic intermediates of phthalate by Tn5 mutant o Pseudomonas teststeroni and analysis of the 4,5-dihydroxyphthalate decarboxylase gene" J.Ferment.Bioeng.77. 583-590 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Lee,J.-H.et al.: "Identification of the plasmid-encoded 4,5-dihydro-4,5-dihydroxyphthalate dehydrogenase gene of Pseudomonas teststeroni involved in metabolism" J.Ferment.Bioeng.78. 462-465 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Konohana,T.et al.: "Nitrate reduction in Bacillus licheniformis in the presence of ammonium salt by shaking culture" Biosci.Biotech.Biochem.57. 1082-1086 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kawai,F.et al.: "Proposed mechanism for bacterial degradation of polyacrylate" J.Environ.Polym.Degr.2. 59-65 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kita,K.et al.: "Degradation of poly (3-hydroxybutyrate),PHB,by marine bacteria and properties of PHB depolymerase from an isolate,Alcaligenes faecalis AE122" Appl.Environ.Microbiol.61. in press (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kikuchi,Y.et al.: "Identification of the bphA4 gene encoding ferredoxin reductase involved in the biphenyl and polychlorinated biphenyl degradation in Pseudomonas sp.strain KKS102" J.Bacteriol.176. 1689-1694 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Nagata,Y.et al.: "Cloning and sequencing of a 2,5-dichloro-2,5-cyclohexadiene-1,4-diol dehydrogenase involced in the degradation of γ-hexachlorocyclohexane in Pseudomonas paucimobilis" J.Bacteriol.176. 3117-3125 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Fukuda,M.et al.: "Identification of the bphyA and bphB genes of Pseudomonas sp.strain KKS102 involved in degradation of polychlorinated biphenyls" Biochem.Biophys.Res.Commun.202. 850-856 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Furukawa,K.et al.: "Efficient degradation of trichloroethylene by a hybrid aromatic ring dioxygenase" J.Bacteriol.176. 2121-2123 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Hirose,J.et al.: "Construction of hybrid biphenyl (bph) and toluene (tod) genes for functional analysis of aromatic ring dioxygenases" Gene. 138. 27-33 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kato,J.et al.: "Cloning and characterization of a Pseudomonas aeruginosa gene involved in the negative regulation of phosphate taxis" J.Bacteriol.176. 5874-5877 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Uchiyama,H.et al.: "Immobilization of trichloroethylene-degrading bacterium,Methylocystis sp.strain M in different matrices" J.Ferment.Bioeng.77. 173-177 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Lee, J.-H.et al.: "Identification of the metabolic intermediates of phthalate by Tn 5 mutant of Pseudomonas teststeroni and analysis of the 4,5-dihydroxyphthalate decarboxylase gene" J.Ferment.Bioeng.77. 583-590 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Lee, J.-H.et al.: "Identification of the plasmid-encoded 4,5-dihydro-4,5-dihydroxyphthalate dehydrogenase gene of Pseudomonas teststeroni involved in metabolism" J.Ferment.Bioeng.78. 462-465 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Konohana, T.et al.: "Nitrate reduction in Bacillus licheniformis in the presence of ammonium salt by shaking culture" Biosci.Biotech.Biochem.57. 1082-1086 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kawai, F.et al.: "Proposed mechanism for bacterial degradation of polyacrylate" J.Environ.Polym.Degr.2. 59-65 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kita, K.et al.: "Degradation of poly (3-hydroxybutyrate), PHB,by marine bacteria and properties of PHB depolymerase from an isolate, Alcaligenes faecalis AE122" Appl.Environ.Microbiol.61 (in press). (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kikuchi, Y.et al.: "Identification of the bphA4 gene encoding ferredoxin reductase involved in the hybrid and polychlorinated biphenyl degradation in Pseudomonas sp.strain KKS102" J.Bacteriol.176. 1689-1694 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Nagata, Y.et al.: "Cloning and sequencing of a 2,5-dichloro-2,5-cyclohexadiene-1,4-diol dehydrogenase involced in the degradation of gamma-hexachlorocyclohexane in Pseudomonas paucimobilis" J.Bacteriol.176. 3117-3125 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Fukuda, M.et al.: "Identification of the bphA and bphB genes of Pseudomonas sp.strain KKS102 involved in degradation of polychlorinated biphenyls" Biochem.Biophys.Res.Commun.202. 850-856 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Furukawa, K.et al.: "Efficient degradation of trichloroethylene by a hybrid aromatic ring dioxygenase" J.Bacteriol.176. 2121-2123 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Hirose, J.et al.: "Construction of hybrid biphenyl (bph) and toluene (ted) genes for functional analysis of aromatic ring dioxygenases" Gene. 138. 27-33 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kato, J.et al.: "Cloning and characterization of a Pseudomonas aeruginosa gene involved in the negative regulation of phosphate taxis" J.Bacteriol.176. 5874-5877 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Uchiyama, H.et al.: "Immobilization of trichloroethylene-degrading bacterium, Methylocystis sp.strain M in different matrices" J.Ferment.Bioeng.77. 173-177 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Lee,J.-H.et al.: "Identification of the metabolic intermediates of phthalate by Tn5mutants of Pseudomoas teststeroni and analysis of the 4,5-dihydroxythalate decarboxylase gene" J.Ferment.Bioeng.77. 583-590 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Lee,J.-H.et al.: "Identification of the plasmid-encoded 4,5-dihydro-4,5-dihydroxyphthalate dehydrogenase gene of Pseudomonas testtseroni involved in metabolism" J.Ferment.Bioeng.78. 462-465 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Konohana,T.et al.: "Nitrate reduction in Bacillus licheniformis in the presence of ammonium salt by shaking culture" Biosci.Biotech.Biochem.57. 1082-1086 (1993)

    • Related Report
      1994 Annual Research Report
  • [Publications] Kawai,F. et al.: "Proposed mechanism for bacterial degradation of polyacrylate" J.Environ.Polym.Degr.2. 59-65 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Kita,K.et al.: "Degradation of poly(3-hydroxybutyrate),PHB,by marine bacteria and properties of PHB depolymerase from an isolate,Alcaligenes faecalis AE122" Appl.Environ.Microbiol.61(in press). (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] Kikuchi,Y.et al.: "Identification of the bphA4 gene encoding ferredoxin reductase involved in the biphenyl and polychlorinated biphenyl degradation Pseudomonas sp.strain KKS102" J.Bacteriol.176. 1689-1694 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Nagata,Y.et al.: "Cloning and sequencing of a 2,5-dichloro-2,5-cyclohexadiene-1,4-diol dehydrogenase involced in the degradation of γ-hexachlorocyclohexane in Pseudomonas paucimobilis" J.Bacteriol.176. 3117-3125 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Fukuda,M.et al.: "Identification of the bphA and bphB genes of Pseudomonas sp.strain KKS102 involved in degradation of polychlorinated biphenyls" Biochem.Biophys.Res.Commun.202. 850-856 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Furukawa,K.et al.: "Efficient degradation of trichloroethylene by a hybrid aromatic ring dioxygenase" J.Bacteriol.176. 2121-2123 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Hirose,J.et al.: "Construction of hybrid biphenyl(bph)and toluene(tod)genes for functional analysis of aromatic ring dioxygenases" Gene. 138. 27-33 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Kato,J.et al.: "Cloning and characterization of a Pseudomonas aeruginosa gene involved in the negative regulation of phosphate tais" J.Bacteriol.176. 5874-5877 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Uchiyama,H.et al.: "Immobilization of trichloroethylene-degrading bacterium,Methylocystis sp.strain M in different matrices" J.Ferment.Bioeng.77. 173-177 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Ouchiyama,N.: "Biodegradation of carbazole by Pseudomonas spp.CA06 and CA10" Biosci.Biotech.Biochem.57. 455-460 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Komatsu,T.: "Microbial degradation of the polycyclic aromatic hydrocarbons acenaphthene and acenaphtylene by a pure bacterial culture" Biosci.Biotech.Biochem.57. 864-865 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Konohana,T.: "Nitrate reduction in Bacillus licheniformis in the presence of ammonium salt by shaking culture" Biosci.Biotech.Biochem.57. 1082-1086 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Kawai,F.: "Bacterial degradation of acrylic oligomers and polymers" Appl.Microbiol.Biotechnol.39. 382-385 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Ohshima,T.: "Cloning and sequencing of a gene encoding nitrite reductase from Paracoccus denitrificans and expression of the gene in Escherichia coli" J.Ferment.Bioeng.76. 82-88 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Nagata,Y.: "Isolation and characterization of Tn5-induced mutants of Pseudomonas paucimobilis UT26 defective in γ-hexachlorocyclohexane dehydrochlorinase(LinA)" Biosci.Biotech.Biochem.57. 703-709 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Nagata,Y.: "Purification and characterization of γ-hexachlorocyclohexane(γ-HCH)dehydrochlorinase(LinA)from Pseudomonas paucimobilis" Biosci.Biotech.Biochem.57. 1582-1583 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Nagata,Y.: "Cloning and sequencing of dehalogenase gene encoding an enzyme with hydrolase activity involved in the degradation of γ-hexachlorocyclohexane(γ-HCH)in Pseudomonas paucimobilis" J.Bacteriol.175. 6403-6410 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Furukawa,K.: "Gene components responsible for discrete substrate specificity in the catabolism of biphenyl(bph operon)and toluene(tod operon)" J.Bacteriol.175. 5224-5232 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Yamamoto,K.: "Kinetics and modeling of hexavalent chromium reduction in Enterobacter cloacae" Biotechnol.Bioeng.41. 129-133 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Kato,J.: "Genetic improvement of Escherichia coli for the enhanced biological removal of phosphate from wastewaters" Appl.Environ.Microbiol.59. 3744-3749 (1993)

    • Related Report
      1993 Annual Research Report

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Published: 1993-04-01   Modified: 2016-04-21  

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