| Project/Area Number |
05304059
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| Research Category |
Grant-in-Aid for Co-operative Research (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
Laboratory animal science
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| Research Institution | The University of Tokyo |
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Principal Investigator |
MITSUOKA Tomotari The Univ.of Tokyo, Fac.of Agriculture, Homorary Prof., 農学部, 名誉教授 (30157549)
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| Co-Investigator(Kenkyū-buntansha) |
HIRAYAMA Kazuhiro The Univ.of Tokyo, Fac.of Agriculture, Assist.Prof., 農学部, 助手 (60208858)
ITOH Kikuji The Univ.of Tokyo, Fac.of Agriculture, Assoc.Prof., 農学部, 助教授 (50100045)
HIOKI Kyouji Central Institute for Experimental Animals Research Head, 室長 (80208735)
ITOH Toshio Central Institute for Experimental Animals Research Head, 室長 (20106644)
NOMURA Tatsuji Central Institute for Experimental Animals, President, 所長 (10072399)
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| Project Period (FY) |
1993 – 1995
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| Project Status |
Completed (Fiscal Year 1995)
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| Budget Amount *help |
¥20,700,000 (Direct Cost: ¥20,700,000)
Fiscal Year 1995: ¥4,300,000 (Direct Cost: ¥4,300,000)
Fiscal Year 1994: ¥5,000,000 (Direct Cost: ¥5,000,000)
Fiscal Year 1993: ¥11,400,000 (Direct Cost: ¥11,400,000)
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| Keywords | Gnotobiote / C-Ha-ras / DMH / Colon cancer / Bile acids / Transgenic mice / トランスジェニック マウス / 発ガン / Clostridium paraputriticum |
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| Research Abstract |
Gnotobiotic transgenic mice with human cancer gene, c-Ha-ras gene, were Produced by oral inoculation with various intestinal bacteria isolated from human and mouse feces. We compared the colon tumor incidence after subcutaneous injection with dymetilhydrazin among various types of gnotobiotic mice. The tumor incidence of transgenic mice was higher than control mice in each group. The differences of floral composition affected the tumor incidence. In some cases floral bacteria stimulated the formation of tumors and in some cases inhibited. there were no relationship among tumor incidence, beta-glucuronidase activity and production of secondary bile acids.
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