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Extensive studies on the dynamic aspects of myofilament sliding in muscle as a micromachine.

Research Project

Project/Area Number 05305007
Research Category

Grant-in-Aid for Co-operative Research (A)

Allocation TypeSingle-year Grants
Research Field 広領域
Research InstitutionSchool of Medicine, Teikyo University

Principal Investigator

SUGI Haruo  Teikyo Univ.Sch.Med.Professor, 医学部, 教授 (20082076)

Co-Investigator(Kenkyū-buntansha) SUTOH Kazuo  Univ.Tokyo, Fac.Lib.Arts Assoc.Prof., 教養学部, 助教授 (20111453)
SUGA Hiroyuki  Okayama Univ., Sch.Med.Professor, 医学部, 教授 (90014117)
SAEKI Yasutake  Tsurumi Univ.Sch.Dent.Assoc.Prof., 歯学部, 助教授 (20046113)
ISHIWATA Shinichi  Waseda, Univ.Sch.Tech.Professor, 理工学部, 教授 (10130866)
YAMADA Kazuhiro  Oita Med.College Professor, 教授 (20053027)
茶圓 茂  帝京大学, 医学部, 講師 (60142452)
山田 武範  帝京大学, 医学部, 助教授 (50027330)
Project Period (FY) 1993 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥20,200,000 (Direct Cost: ¥20,200,000)
Fiscal Year 1995: ¥4,000,000 (Direct Cost: ¥4,000,000)
Fiscal Year 1994: ¥6,000,000 (Direct Cost: ¥6,000,000)
Fiscal Year 1993: ¥10,200,000 (Direct Cost: ¥10,200,000)
KeywordsMuscle contraction / Myofilament / Actomyosin / Santan-clothing
Research Abstract

The results obtained are summarized as follows.
1.Measurement of the unitary distance of actin-myosin sliding in muscle : Yamada, Sugi and others showed that a glycerinated skeletal muscle fiber shortens uniformly along its entire length by 10 nm/half sarcomere, when it is activated by laser flash photolysis of caged ATP releasing ATP molecules whose number was equal to the total number of myosin molecules within the fiber. Analysis of the flash-induced fiber shortening indicated that the internal resistance against shortening was nearly the same as the maximum isometric force, suggesting that the unitary distance of actin-myosin sliding is about 10 nm in the isometric condition.
2.Function of myosin motor domains as studied by techniques of genetic engineering : Sutoh and others succeeded in preparing mutant myosins from a slime mold, Dictyostelium, and found (1) a segment of myosin subfragment 1 (S-1) consisting only of 760 amino acid residues could still generate sliding force with actin filaments, and (2) mutant myosins, in which amino acid residues are lacking in the subfragment 2 (S-2) region, could slide actin filaments past them with velocities slower than those for control myosin, suggesting the involvement of S-2 in muscle contraction.
3.Development of techniques manipulating single molecules : Kinosita and others developed a light microscope system, with which single fluorosphores could be clearly observed as a result of reduced background fluorescence. This system is extremely useful for studying the mechanochemical reaction between single myosin and single ATP molecules. Ando and others, on the other hand, succeeded in combining an atomic force microscope with a fluorescent microscope, also providing the system for studying the single molecule dynamics of actomyosin-ATP system.

Report

(4 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • 1993 Annual Research Report
  • Research Products

    (35 results)

All Other

All Publications (35 results)

  • [Publications] Yamada,T.,Abe,O.,Kobayashi,T.& Sugi,H: "Myofilament sliding per ATP molecule in rabbit muscle fibres studied using laser flash photolysis of caged ATP" Journal of Physiology. 466. 229-243 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Sugi,H.,Oiwa,K.& Chaen,S.: "Dependence of the work done by ATP-induced actin-myosin sliding on the initial baseline force" Advances in Experimental Medicine and Biology,ed.by.Sugi,H.& Pollack G.H.332. 303-311 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Chaen,S.,Inoue,J.& Sugi,H.: "The force-velocity relationship of the ATP-dependent actin-myosin sliding causing cytoplasmic streaming in algal cells" Journal of Experimenatal Biology. 198. 1021-1027 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Nishizaka,T.,Yagi,T.,Tanaka,Y.& Ishiwata,S.: "Right-handed relation of an actin filament in an in vitro motile system" Nature. 361. 269-271 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Yamamoto,T.et al.: "Determination of electrostatic potential around specific locations on the surface of actin by diffusion-enhanced fluorescence energy transfer" Journal of Molecular Biology. 241. 714-731 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Fisher,A.J.et al.: "X-ray structure of the myosin motor domain of Dictyostelium discoideum complexed with Mg・ADP・Bef and Mg・ADP・ALF4" Biochemistry. 34. 8960-8972 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Sugi,H.& Pollack,G.H.: "Mechanism of Myofilament Sliding in Muscle Contraction" Plenum,New York, 866 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 杉 晴夫,斉藤 望,佐藤昭夫: "運動生理学" 南江堂、東京, 121 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Yamada, T., Abe.O., Kobayashi T.& Sugi, H.: "Myofilament sliding per ATP molecule in rabit muscle fibers studied using laser flash photolysis of caged ATP." Journal of Physiology. 466. 229-243 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Sugi, H., Oiwa, K.& Chaen, S.: "Dependence of the work done by ATP-nduced actin-myosin sliding on the initial baseline force" Advances in Experimental Medicine and Biology. 322. 303-311 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Chaen, S., Inoue, J.& Sugi, H.: "The force-velocity relationship of the ATP-dependent actin-myosin sliding causing cytoplasmic streaming in algal cells." Journal of Experimental Biology. 198. 1021-1027 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Nishizaka, T., Yagi, T., Tanaka, Y.& Ishiwata, S.: "Right-handed rotation of an actin filament in an in vitro motile system." Nature. 361. 269-271 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Itakura, S., Yamakawa, H., Toyoshima, Y., Ishijima, A., Kojima, T., Harada, Y., Yanagida, T., Wakabayashi, T.& Sutoh, K.: "Force-generating domain of myosin motor" Biochemical and Biophysical Research Communication. 196. 1504-1510 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Fisher, A.J., Smith, C.A., Thoden, J., Smith, R., Sutoh, K., Holden, H.M.& Rayment, I.: "X-ray structure of the myosin motor domain of Dictyostelium discoideum complexed with Mg・ADP・BeF and Mg・ADP・ALF4." Biochemistry. 34. 8960-8672 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Yamamoto, T., Nakayama, S., Kobayashi N., Munekata, E.& Ando, T.: "Determination of electrostatic potential around specific locations on the surface of actin by diffusion-enhanced fluorescence energy transfer." Journal of Molecular Biology. 241. 714-731 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Sugiura, S. et al.: "Force-velocity relations of rat cardiac myosin isozymes sliding on algal cell actin cables in vitro" Biochimica et Biophysica Acta. 1231. 69-75 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Chaen, S. Inoue, J. & Sugi, H.: "The force-velocity relationship of the ATP-dependent actin-myosin sliding causing cytoplasmic streaming in algal cells" Journal of Experimental Biology. 198. 1021-1027 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Katayama, E., Scott-Woo, G. & Ikebe, M.: "Effect of caldesmon on the assembly of smooth muscle myosin" Journal of Biological Chemistry. 270. 3919-3925 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Onishi, H. et al.: "Functional chicken gizzard heavy meromyosin expression in and purification from baculovirus-infected insect cells" Proceedings of the National Academy of Sciences, USA. 92. 704-708 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Watanabe, S. et al.: "Temperature acclimation induces light meromyosin isoforms with different primary structures in carp fast skeletal muscle" Biochemical and Biophysical Research Communications. 208. 118-125 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Kagawa, K. Horiuti, K. & Yamada, K.: "BDM compared to Pi and low Ca^<2+> in the cross-bridge reaction initiated by flash photolysis of carged ATP" Biophysical Journal. 69. 2590-2600 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Oishi,N.&Sugi,H.: "In vitro ATP-dependent F-actin sliding on myosin is not influenced by substitution or removal of bound nucleotide." Biochimica et Biophysica Acta. 1185. 346-349 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Miyata,H.&Kinosita,K.: "Transformation of actin-encapsulating Iiposomes by cytochalasin D and accompanying change in alignm ent of actin filaments." Biophysical Journal. 67. 922-928 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Wakabayashi,K.et al.: "X-ray diffraction evidence for the extensibility of actin and myosin filaments during muscle contraction." Biophysical Journal. 67. 2422-2435 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Miyata,H.et al.: "Stepwize motion of an actin filament over a small number of heavy meromyosin molecules in an in vitro motility assay" Journal of Biochemistry. 115. 644-647 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Komatsu,H.&Tawada,K.: "Trinitrophylation of the reactive lysine residue in double -headed myosin in the presence of PPi." Journal of Biochemistry. 115. 1190-1196 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Chaen,S.et al.: "The force-velocity relationship of the ATP-dependent action-myosin sliding causing cytoplasmic streaming in algal cells." Journal of Experimental BIology. 198(in press). (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] 杉晴夫.斎藤望.佐藤昭夫: "運動生理学" 南江堂, 117 (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] Yamada,T.,Abe,O.,Kobayashi,T.and Sugi,H.: "Myofilament sliding per ATP molecule In rabbit muscle fibres studied using laser flash photolysis of caged ATP" Journal of Physiology(London). 466. 229-243 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Yamada,T.,Kikuchi,K.,and Sugi,H.: "^<31>PNuclear magnetic resonance studies on the glycogenolysis regulation in resting and contracting frog skeletal muscle" Journal of Physiology(London). 460. 273-286 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Oiwa,K.,Kawakami,T.,and Sugi,H.: "Unitary distance of actin-myosin sliding studied using an in vitro force-movement assay system combined with ATP iontophoresis" Journal of Biochemistry. 114. 28-32 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Phillips,K.,Takei,M.and K.Yamada: "Time course of phosphate metabolites and intracellular pH using ^<31>PNMR compared to recovery heat in rat soleus" Journal of Physiology(London). 460. 693-704 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Nishizaka,T.,Yagi,T.,Tanaka,Y.and Ishiwata,S.: "Right handed rotation of an actin filament in an in vitro motile system" Nature(London). 361. 269-271 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Saeki,Y.,Kurihara,S.,Hongo,K.and Tanaka,E.: "Alterations in intracellular calcium and tension of activated ferret papillary muscle in response to step length changes" 463. 291-306 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Sugi,H.and Pollack,G.H.: "Mechanism of Myofilament Sliding in Muscle Contraction" Plenum Press,New York, 866 (1993)

    • Related Report
      1993 Annual Research Report

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Published: 1993-04-01   Modified: 2016-04-21  

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