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Tertiary structure and molecular modeling of insect neuropeptides

Research Project

Project/Area Number 05403031
Research Category

Grant-in-Aid for General Scientific Research (A)

Allocation TypeSingle-year Grants
Research Field Applied molecular and cellular biology
Research InstitutionThe University of Tokyo

Principal Investigator

SUZUKI Akinori  The University of Tokyo, Faculty Agriculture, Professor, 農学部, 教授 (90011907)

Co-Investigator(Kenkyū-buntansha) KATAOKA Hiroshi  The University of Tokyo, Faculty of Agriculture, Associate Professor, 農学部, 助教授 (60202008)
Project Period (FY) 1993 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥38,800,000 (Direct Cost: ¥38,800,000)
Fiscal Year 1995: ¥7,000,000 (Direct Cost: ¥7,000,000)
Fiscal Year 1994: ¥14,700,000 (Direct Cost: ¥14,700,000)
Fiscal Year 1993: ¥17,100,000 (Direct Cost: ¥17,100,000)
KeywordsNMR / Tertiary structure / PTTH / Insulin / インスリン族ペプチド / ボンビキシン / 昆虫神経ペプチド / 前胸腺刺激ホルモン / 昆虫 / ペプチド
Research Abstract

1.The tertiary structure of bombyxin was determined by the NMR method.
2.From the bioassay of chimeric molecules of bombyxin and insulin, the central part of B-chain, TyrB6-LeuB18, is found to be very important for bombyxin activity.
3.Solution structure determination of these chimeric molecules revealed that their B-chain central parts took similar main-chain conformation, but formed dissimilar patches on their molecular surface. Therefore, the surface patch formed by the central part of the bombyxin B-chain is of critical importance for recognition of bombyxin receptor.
4.NMR data of PTTH revealed that the central part of PTTH has rigid structure and is rich in beta-sheet.
5.Molecular modeling of PTTH by computer suggested that PTTH has two b-sheets and is belong to the cystine knot superfamily.

Report

(4 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • 1993 Annual Research Report
  • Research Products

    (17 results)

All Other

All Publications (17 results)

  • [Publications] J.Ishibashi: "Assignment of disulfide bond location in prothoracicotropic hormone (PTTH) of the silkworm,Bombyx mori,a heterodimeric peptide." Biochemistry. 33. 5912-5919 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] K.Nagata: "Three-dimensional structure of bombyxin-II,an insulin-like peptide of the silkmoth Bombyx mori. Structural comparison with insulin and relaxin." J. Mol. Biol.253. 749-758 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] K.Nagata: "Identification of Receptor-recognition surface of bombyxin-II,an insulin-like peptide of the silkmoth Bombyx mori. Critical importance of the B-chain central part." J. Mol. Biol.253. 759-770 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] T.Noguchi: "Three-dimensional structure and receptor-recognition surface of Bombyxin-II,an insulin-related peptide of the silkmoth Bombyx mori." FEBS Letter. 376. 251-256 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Nagata: "Insect prothoracocotropic hormone : a new menber of the vertebrate growth factor superfamily." Peptide Chemistry 1994. 85-88 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] J.Ishibashi: "Assignment of disulfide bond location in prothoracicotropic hormone (PTTH) of the silkworm, Bombyx mori, a heterodimeric peptide." Biochemistry. 33. 5912-5919 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] K.Nagata: "Three-dimensional structure of bombyxin-II,an insulin-like peptide of the silkmoth Bombyx mori. Structural comparison with insulin and relaxin." J.Mol.Biol.253. 749-758 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] K.Nagata: "Identification of Receptor-recognition surface of bombyxin-II,an insulin-like peptide of the silkmoth Bombyx mori. Critical importance of the B-chain central part." J.Mol.Biol.253. 759-770 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] T.Noguchi: "Insect prothoracocotropic hormone : a new menber of the vertebrate growth factor superfamily." FEBS Letter. 376. 251-256 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] K.Nagata: "Three-dimensional structure and receptor-recognition surface of Bombyxin-II,an insulin-related peptide of the silkmoth Bombyx mori." Peptide Chemistry. 1994. 85-88 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Nagata,K.et al: "Three-dimensional solution structure of Bombyxin-II an insulin-like peptide of the silkmoth Bombyx mori:Structure Comparison with Insulin and Relaxin" J.Mol.Biol.253. 749-758 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Nagata,K.et al: "Identification of the receptor-Recognition Surface of Bombyxin II,an insulin-like peptide of the Silkmoth Bombyx mori:Critical importance of the B-chain center Part." J.Mol.Biol.253. 759-770 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Noguchi,T.et al: "Insect prothoracicotropic hormone:a new member of the vertebrate growth factor superfamily" FEBS Letter. 376. 251-256 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] J.Ishibashi et.al.: "Aszignment of disulficle fond location in prothoraciiotropic lormone of silkrorm Bombyx.mori : a homo climetic poptide" Biochemistry. 33. 5912-5919 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] K.Nagata et al.: "Three-dimensional structure and receptor-recognition surface of bomlyxin-II,an insuliu-telated peptide of the silkmoth Bembyx mori" Peptide Chemistry 1994. (in press). (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] J.Ishibashi: "Determination of disulfide bond location of Bombyx prothoracicotropic hormone,a homodimeric peptide." Peptide Chemistry 1992. 568-571 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] K.Nagata: "Insulin,bombyxin and their hybrids:Synthesis and activity." Peptide Chemistry 1992. 416-419 (1993)

    • Related Report
      1993 Annual Research Report

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Published: 1993-04-01   Modified: 2016-04-21  

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