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The Research on the Production of Usefull Compounds by the Control of Regulations in the Biosynthesis of Secondary Metabolism

Research Project

Project/Area Number 05403034
Research Category

Grant-in-Aid for General Scientific Research (A)

Allocation TypeSingle-year Grants
Research Field Bioorganic chemistry
Research InstitutionUniversity of Tokyo

Principal Investigator

SANKAWA Ushio  University of Tokyo, Faculty of Pharmaceutical Sciences, Professor, 薬学部, 教授 (60012613)

Co-Investigator(Kenkyū-buntansha) HAKAMATSUKA Takashi  University of Tokyo, Faculty of Pharmaceutical Sciences, Assistant Professor, 薬学部, 教務職員 (60221488)
SHIBUYA Masaaki  University of Tokyo, Faculty of Pharmaceutical Sciences, Assistant Professor, 薬学部, 助手 (50170923)
FUJII Isao  University of Tokyo, Faculty of Pharmaceutical Sciences, Assistant Professor, 薬学部, 助手 (70181302)
NOGUCHI Hiroshi  University of Tokyo, Faculty of Pharmaceutical Sciences, Lecturer, 薬学部, 講師 (60126141)
Project Period (FY) 1993 – 1994
Project Status Completed (Fiscal Year 1994)
Budget Amount *help
¥38,900,000 (Direct Cost: ¥38,900,000)
Fiscal Year 1994: ¥15,800,000 (Direct Cost: ¥15,800,000)
Fiscal Year 1993: ¥23,100,000 (Direct Cost: ¥23,100,000)
KeywordsBiosynthesis / Regulation of Metabolism / Production of Usefull compounds / Chalcone Synthase / Flavonoids / Elicitor / Transformation of Plant / Secondary Metabolism of Microorganism / カルコン合成酵 / 二次代謝 / カルコン合成還元酵素 / オキシドスクアレン閉環酵素 / パーオキシダーゼプロモーター / ザルコトキシン
Research Abstract

(1) The genomic gene encoding chalcone synthase which was the key enzyme of flavonoid biosynthesis was cloned from cultured Pueraria lobata cells. And also the three genomic clones of reductase which was co-acting with chalcone synthase during the formation of deoxychalcone formation were isolated from P.lobata. Since chalcone synthase and reductase are acting together, the promoter regions of chalcone synthase and reductase genes are supposed to be similar. However they have no over-all similarity, but have several similar sequences in the length of about 20 bp. The purification of 2-hydroxyisoflavone dehydratase was achieved in this period and the cloning of its cDNA is proceeding.
(2) The response of cultured P.lobata cells to elicitors such as yeast extracts, jasmonic acid and CuCl_2 was investigated at compound level, enzyme level and RNA level. The results clearly showed that yeast extracts and jasmonic acid responsed very fast at any level, on the other hand CuCl_2 responsed slowly.
(3) The chimeric gene of the promoter region of horse radish peroxidase gene and Sarcotoxin cDNA was introduced to Tobacco plant using the Agrobacterium and Ti-Plasmid system to produce the plant having the anti-batcerial activity.
(4) The gene encoding dihydrogeodin oxygenase which catalyzed intramolecular phenol oxidative coupling reaction was cloned from Aspergillus terreus. The coned gene was transformed to Aspergillus nidulans and expressed in the enzymatically active form in large scale.

Report

(3 results)
  • 1994 Annual Research Report   Final Research Report Summary
  • 1993 Annual Research Report
  • Research Products

    (23 results)

All Other

All Publications (23 results)

  • [Publications] Kyung-Sik Song他: "Elicitors Which Induce the Accumulation of p-Coumary/Amino Acids in Ephedra distachya Cultures" Arch.Pharm.Res.17. 26-30 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Naohiro Tsukamoto 他: "Nucleotide Sequence of akn A Region of the Aklavinone Biostnthesis Gene Cluster of Streptomyces galilaeus" J.Bacteriology. 176. 2473-2475 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Takashi Hakamatsuka 他: "Pueraria lobata(kuza vine):In Vitro Culture and the Production of Isoflavonoids" Biotechnology in Agriculture and Forestry 28 Medicinal Aromatic Plant. VII. 386-400 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Masayuki Kusano 他: "Cloning of cDNA Encoding Rat 2,3-Oxidosqualene:Lanosterol Cyclase" Biol.Pharm.Bull. 18. 195-197 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Hyung-Hwang Park 他: "Rapid Metabolism of Isoflavonoids in Elicitor Treated Cell Suspention" Phytochemistry. (in press). (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Zhi-Gang Chen 他: "Purification and Characterization of Einodinan thone Oxygenase" Phytochemistry. (in press). (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kyung-Sik Song: "Ushio Sankawa and Yutaka Ebizuka, Elicitors Which Induce the Accumulation of p-Coumaroyl Amino Acids in Ephedra distachya Cultures" Arch.Pharm.Res.17. 26-30 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Naohiro Tsukamoto, Isao Fujii, Yutaka Ebizuka and Ushio Sankawa: "Nucleotide Sequence of the aknA Region of the Aklavinone Biosynthesis Gene Cluster of Streptomyces galilaeus" J.Bacteriology. 176. 2473-2475 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Takashi Hakamatsuka, Yutaka Ebizuka and Ushio Sankawa: "Pueraria lobata (Kudzu vine) : In Vitro Culture and the Production of Isoflavonoids, Biotechnology in Agriculture and Forestry 28, Medicinal Aromatic Plants VII.Ed. by Y.P.S.Bajaj" Springer-Verlag. 386-400 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Masayuki Kusano, Masaaki Shibuya, Ushio Sankawa and Yutaka Ebizuka: "Molecular Cloning of cDNA Encoding Rat 2,3-Oxidosqualene : Lanosterol Cyclase" Biol.Pharm.Bull.18. 195-197 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Hyung-Hwang Park, Takashi Hakamatsuka, Ushio Sankawa and Yutaka Ebizuka: "Rapid Metabolism of Isoflavonoids in Elicitor Treated Cell Suspension Cultures of Pueraria lobata" Phytochemistry. (in press). (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Zi-Gang Chen, Isao Fujii, Yutaka Ebizuka and Ushio Sankawa: "Purification and Characterization of Emodinanthrone Oxygenase from Aspergillus terreus" Phytochemistry. (in press). (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kyung-Sik Song 他: "Elicitors Which Induce the Accumulation of p-Coumaryl Amino Acids in Ephedra distachya Cultures" Arch.Pham.Res.17. 26-30 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Naohiro Tsukamoto 他: "Nucleotide Sequence of akn A Region of the Aklavinone Biosynthesis Gene Cluster of Streptomyces galilaeus" J.Bacteriology. 176. 2473-2475 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Takashi Hakamatsuka 他: "Pueraria lobata(knzu vine):In Vitro Culture and the Production of Isoflavonoids" Biotechnology in Agriculture and Foresery 28,Medictial Aromatic Plant. VII. 386-400 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Masayuki Kusano 他: "Cloning of cDNA Encoding Rat 2,3-Oxidosqualene:Lanosterol Cyclase" Biol/Pharm.Bull.18. 195-197 (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] Hyung-Hwang Park 他: "Rapid Metabolism of Isoflavonoids in Elicitor Treated Cell Suspension" Phytochemistry. (in press). (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] Zhi-Gang Chen 他: "Purification and Characterization of Einodinanehome Oxygenase" Phytochemistry. (in press). (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] 渋谷 雅明 他: "Probing Ergot Alkaloid Biosynthesis" J.Amer Chem.Soc.115. 2482-2455 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 三川 潮 他: "Recent Progress in Studies of the Biosynthesis of Isoflavonoids" J.Plant Research.Special Issue No3. 129-144 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 三川 潮 他: "Post-Aromatic Deoxygenation in Polyketide Biosynthesis" Chem.Pharm.Bull. 41. 2015-2021 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 三川 潮: "薬用植物と医薬品開発" 植物細胞工学. 5(印刷中). (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] 渋谷 雅明 他: "抗腫瘍性植物成分" 植物細胞工学. 5(印刷中). (1994)

    • Related Report
      1993 Annual Research Report

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Published: 1993-04-01   Modified: 2016-04-21  

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