| Project/Area Number |
05404005
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| Research Category |
Grant-in-Aid for General Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
植物生理
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| Research Institution | Himeji Institute of Technology |
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Principal Investigator |
SHIMMEN Teruo Himeji Institute of Technology, Faculty of Science, Professor, 理学部, 教授 (80114510)
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| Co-Investigator(Kenkyū-buntansha) |
YOKOTA Etsuo Himeji Institute of Technology, Faculty of Science, Assistant Professor, 理学部, 助手 (80212299)
三村 徹郎 姫路工業大学, 理学部, 助教授
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| Project Period (FY) |
1993 – 1995
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| Project Status |
Completed (Fiscal Year 1995)
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| Budget Amount *help |
¥33,600,000 (Direct Cost: ¥33,600,000)
Fiscal Year 1995: ¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 1994: ¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 1993: ¥28,100,000 (Direct Cost: ¥28,100,000)
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| Keywords | pollen tube / myosin / actin / actin-binding protein / calcium / lily / cytoplasmic streaming / root hair cell / サイトカラシン / Mimura, T.他3名 |
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| Research Abstract |
1) Purification and characterization of myosin : We succeeded in purifying myosin from pollen tubes of lily. Molecular weight of the myosin was about 150 kDa. This is the first success in purifying myosin showing sliding activity along actin filaments from plants. ATPase and sliding activity along actin filaments was inhibited by calcium. It is generally accepted that cytoplasmic streaming is inhibited by calcium in plant cells. Inhibition of myosin activity demonstrated in the present study is the molecular basis of calcium regulation of cytoplasmic streaming. We also succeeded in preparing antibody against this myosin. Using this myosin antibody, we analyzed distribution of this myosin in various plant cells.
2) Reverse fountain streaming : In root hair cells of Hydrocharis, we can observed reverse fountain streaming. Using inhibitors of actin, we unequivocally demonstrated that actin is involved in maintenance of structure of transvacuolar strands and occurrence of cytoplasmic streaming. It was also demonstrated that microtubules are involved in maintenance of organization of actin filaments in the cell.
3) Purification of actin binding protein : It is generally accepted that actin binding proteins are involved in organization of actin filaments. However, studies on actin binding protein is scanty in plant cells. We succeeded in purifying actin bundling protein from pollen tubes of lily. We analyzed various characteristics of the actin-bundling protein. This is the first success in purifying actin-bundling protein from plant cells.
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