• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Studies on Molecular Breeding by Functional Analysis of Rice Mitochondrial Genome

Research Project

Project/Area Number 05404007
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Breeding science
Research InstitutionThe University of Tokyo

Principal Investigator

HIRAI Atsushi  The University of Tokyo, Graduate School of Agricultural and Life Science, Professor, 大学院・農学生命科学研究科, 教授 (60023470)

Co-Investigator(Kenkyū-buntansha) TSUTSUMI Nobuhiro  The University of Tokyo, Graduate School of Agricultural and Life Science, Asso., 大学院・農学生命科学研究科, 助教授 (00202185)
多々良 敦  東京大学, 農学部, 助教授 (60114542)
武田 元吉  東京大学, 農学部, 教授 (90134501)
Project Period (FY) 1993 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥33,500,000 (Direct Cost: ¥33,500,000)
Fiscal Year 1996: ¥4,800,000 (Direct Cost: ¥4,800,000)
Fiscal Year 1995: ¥2,800,000 (Direct Cost: ¥2,800,000)
Fiscal Year 1994: ¥4,400,000 (Direct Cost: ¥4,400,000)
Fiscal Year 1993: ¥21,500,000 (Direct Cost: ¥21,500,000)
KeywordsGene expression / Mitochondrial genome / Rice / transcription / 転写地図 / 反復配列 / PRS
Research Abstract

The mitochondrial genomes of higher plants are much larger and more complex than those of other eukaryotic organisms. The structural complexity of the genomes has been associated with rearrangements among repeated sequences and the presence of many chloroplast-derived sequences. Following evidence shows the mode of gene expression in the mitochondrial genome of rice is also complex and flexible.
Using in vitro capping/ribonuclease protection experiments and primer extension analyzes, we identified the transcription initiation sites of mitochondrial genes. The alignment of sequences surrounding the initiation sites of genes revealed a conserved sequence motif, particularly CRTA motif.
The two gene clusters rps3-rpl16 and nad3-rps12 are separated from each other and are expressed as the individual transcription units in many plants. However, the four genes were located within a region of 6kbp in rice mitochondria, and all four genes were co-transcribed.
The 76 bp sequence found in the upstream region of rrn26 is duplicated in the upstream region of trnfM.It was demonstrated that the transcription of trnfM and rrn26 starts from these sequences in the upstream regions of the respective genes.
A chloroplast-derived tRNA gene, trnH was transcribed in rice mitochondria using mitochondrial specific promoter sequence. On the other hand, the mitochondrial specific gene, nad9 was transcribed together with the chloroplast-derived sequence. It shows a chloroplast-derived sequence is utilized as a promoter for nad9.

Report

(5 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • 1994 Annual Research Report
  • 1993 Annual Research Report
  • Research Products

    (36 results)

All Other

All Publications (36 results)

  • [Publications] Watanabe,N.et al.: "Evolutionary variation in DNA sequences transferred from chloroplast genomes to mitochondrial genomes in the Graminae." Curr.Genet.26. 512-518 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Nakazono,M.et al.: "A small repeated sequence contains the transcription initiation sites for both trnfM and rrn26 in rice mitochondria." Plant Mol.Biol.28. 345-348 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Nakazono,M.et al.: "Multiple initiation sites for transcription of a gene for subunit 1 of F1-ATPase(atp1) in rice mitochondria." Curr. Genet.29. 417-422 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Zeltz,P.et al: "A promiscuous chloropast DNA fragment is transcribed in plant mitochondria but the encoded RNA is not edited." Plant Mol.Biol.31. 647-656 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Nakazono,M.et al.: "A chloroplast-derived sequence is utilized as a source of promoter sequences for the gene for subunit 9 of NADH dehydrogenase (nad9) in rice mitochondria." Mol.Gen.Genet.252. 371-378 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kadowaki,K.et al.: "Targeting presequence acquisiton after mitochondrial gene transfer to the nucleus occurs by duplication of existing targeting signal." EMBO J.15. 6652-6661 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Watanabe, N.et al.: "Evolutionary variation in DNA sequences transferred from chloroplast genomes to mitochondrial genomes in the Graminae." Curr. Genet. 26. 512-518 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Nakazono, M.et al.: "A small repeated sequence contains the transcription initiation sites for both trnfM and rrn26 in rice mitochondria." Plant Mol. Biol.28. 345-348 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Nakazono, M.et al.: "Multiple initiation sites for transcription of a gene for subunit 1 of F1-ATPase (atp1) in rice mitochondria." Curr. Genet.29. 417-422 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Zeltz, P.et al.: "A promiscuous chloropast DNA fragment is transcribed in plant mitochondria but the encoded RNA is not edited." Plant Mol. Biol.31. 647-656 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Nakazono, M.et al.: "A chloroplast-derived sequence is utilized as a source of promoter sequences for the gene for subunit 9 of NADH dehydrogenase (nad9) in rice mitochondria." Mol. Gen. Genet.252. 371-378 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kadowaki, K.et al.: "Targeting presequence acquisiton after mitochondrial gene transfer to the nucleus occurs by duplication of existing targeting signal." EMBO J.15. 6652-6661 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Nakazono,M.et al.: "The gene for a subunit of the ABC-type heme transporter is transcribed together with the gene for subunit 6 of NADH dehydrogenase in rice mitochondria." Curr.Genet.29. 412-416 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Nakazono,M.et al.: "Multiple initiation sites for transcription of a gene for subunit 1 of F1-ATPase(atp1) in rice mitochondria." Curr.Genet.29. 417-422 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Zeltz,P.et al.: "A promiscuous chloropast DNA fragment is transcribed in plant mitochondria but the encoded RNA is not edited." Plant Mol.Biol.31. 647-656 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Nakazono,M:et al.: "A chloroplast-derived sequence is utilized as a source of promoter sequences for the gene for subunit 9 of NADH dehydrogenase (nad9) in rice mitochondria." Mol.Gen.Genet.252. 371-378 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Kadowaki,K.et al.: "Targeting presequence acquisiton after mitochondrial gene transfer to the nucleus occurs by duplication of existing targeting signal" EMBO J.15. 6652-6661 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Miyata,S.et al.: "Four kinds of plasmid-like DNA of rice mitochondria are transcribed." Breed.Sci.47. 51-55 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Itadani,H.et al.: "Nucleotide sequence of a 28 kbp portion of rice mitochondrial DNA:the exitence of many sequences that correspond to parts of mitochondrial genes in intergenic regions." Plant Cell Physiol.35. 1239-1244 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] Nakazono,M.et al.: "The rps3-rpl16-nad3-rps12 gene cluster in rice mitochondrial DNA is transcribed from alternative promoters." Curr.Genet.27. 184-189 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Nakazono,M.et al.: "Nomologous reconmbination mediated by two palindromic repeated sequences in the mitochondrial genome of Oryza." Theor.Appl.Genet.91. 1-8 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Nakazono,M.et al.: "A small repeated sequence contains the transcription initiation sites for both trnfM and rrn 26 in rice mitochondria." Plant Mol.Biol.28. 345-348 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Nakazono,M.et al.: "The CRTA motif is present in the promoters of mitochondrial genes of rice." Palnt Sci.105. 227-234 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Nishiwaki,S.et al.: "Structure of a gene for subunit 9 of NADH dehydrogenase (nad9) in rice mitochondria and RNA editing of its transcript." Plant Cell Physiol.36. 1135-1138 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Watanabe,N.et al.: "Evolutionary variation in DNA sequences transferred from chloroplast genomes to mitochondrial genomes in the Graminae." Curr.Genet.26. 512-518 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Kanazawa,A.,et al.: "Reversible changes in the composition of the population of mtDNAs during dedifferentiation and regeneration in to bacco." Genetics. 138. 865-870 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Itadani,H.,et al.: "Nucleotide sequence of a 28 kbp portion of rice mitochondrial DNA:the existence of many sequences that correspond to parts of mitochondrial genes in intergenic regions." Plant Cell Physiol.35. 1239-1244 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Nakazono,M.et al.: "The rps3-rpl16-nad3-rps12 gene cluster in rice mitochondrial DNA is transcribed from alternative promoters." Curr.Genet.27. 184-189 (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] Nakazono,M.et al.: "Homologous recombination mediated by two palindromic repeated sequences in the mitochondrial genome of Oryza." Theor.Appl.Genet.(in press.).

    • Related Report
      1994 Annual Research Report
  • [Publications] Nakazono,M.et al.: "The CRTA motif is present in the promoters of mitochondrial genes of rice." Plant Sci.(in press.).

    • Related Report
      1994 Annual Research Report
  • [Publications] Kanno,A.: "A transcription map of the chloroplast genome from rice(Oryza sativa)." Curr.Genet.23. 166-174 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Nakazono,M.: "Identification of the entire set of transferred chloroplast DNA sequences in the mitochondrial genome of rice." Mol.Gen.Genet.236. 341-346 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Kanno,A.: "Variations in chloroplast DNA from rice(Oryza sativa):differences between deletions mediated by short direct-repeat sequences within a single species." Theor.Appl.Genet.86. 579-584 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Kanazawa,A.: "Restriction fragments homologous to mitochondrial plasmid-like DNAs are located within limited chromosomal regions on the rice nuclear genome." Theor.Appl.Genet.(in press).

    • Related Report
      1993 Annual Research Report
  • [Publications] Nakazono,M.: "Palindromic repeated sequences(PRSs)in the mitochondrial genome of rice:evidence for their insertion after divergence of the genus Oryza from the other Gramineae." Plant Mol.Biol.(in press.).

    • Related Report
      1993 Annual Research Report
  • [Publications] Hirai,A.: "Six percent of the mitochondrial genome in rice came from chloroplast DNA." Plant Mol.Biol.Reporter. 11. 98-100 (1993)

    • Related Report
      1993 Annual Research Report

URL: 

Published: 1993-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi