| Project/Area Number |
05404030
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| Research Category |
Grant-in-Aid for General Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
Gastroenterology
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| Research Institution | Osaka University |
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Principal Investigator |
KAMADA Takenobu Osaka University Medical School, Professor, 医学部, 教授 (80028399)
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| Co-Investigator(Kenkyū-buntansha) |
MITA Eiji Osaka University Hospital, Medical Staff, 医学部附属病院, 医員
TAKEHARA Tetsuo Osaka University Hospital, Medical Staff, 医学部附属病院, 医員
KATAYAMA Kazuhiro Osaka University Hospital, Medical Staff, 医学部附属病院, 医員
KASAHARA Akinori Osaka University Medical School, Assistant Professor, 医学部, 助手 (70214286)
HAYASHI Norio Osaka University Medical School, Associate Professor, 医学部, 講師 (00144478)
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| Project Period (FY) |
1993 – 1995
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| Project Status |
Completed (Fiscal Year 1995)
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| Budget Amount *help |
¥22,800,000 (Direct Cost: ¥22,800,000)
Fiscal Year 1995: ¥3,900,000 (Direct Cost: ¥3,900,000)
Fiscal Year 1994: ¥7,400,000 (Direct Cost: ¥7,400,000)
Fiscal Year 1993: ¥11,500,000 (Direct Cost: ¥11,500,000)
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| Keywords | in vivo gene transfer / hepatitis C virus / cationic liposome / asialoglycoprotein / rat / liver injury / hepatocarcinogenesis / expression vector / 肝発泡機構 / β-ガラクトシダーゼ / C形肝炎ウィルス / in vivo遺伝子導入 / リポソーム / PCR / 免疫組織化学 / Lac Z / 肝炎 / 肝癌 |
|---|
| Research Abstract |
The lack of small animal model of hepatitis C virus (HCV) infection impeded elucidation of the pathogenesis of this virus. The aim of this study is to develop HCV-expressing model animals using in vivo gene transfer and use them to study the pathogenesis of liver injury and hepatocarcinogenesis. In this study, we used cationic liposome-mediated and asialoglycoprotein receptor-mediatd in vivo gene transfer. To examine the feasibility of these procedures, pActLacZ in which the LacZ gene was driven by the beta-actin promoter was used as an reporter vector and successful transfection into rat hepatocytes in vivo was confermed by X-Gal histochemistry. For expression of HCV,HCV-expression vector in which the full-coding sequence of the HCV genome was driven by the beta-actin promoter was used. The expression of HCV in rat hepatocytes in vivo was confermed by RT/PCR and immunohistochemical staining using anti-HCV core monoclonal antibody. These HCV-expressing model rats will be useful for determining the pathological role of HCV in vivo.
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