| Project/Area Number |
05404048
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Thoracic surgery
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| Research Institution | UNIVERSITY OF TOKYO |
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Principal Investigator |
KAWAUCHI Motohiro Tokyo University Hospital, Assistant Professor, 医学部・附属病院, 講師 (00152918)
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| Co-Investigator(Kenkyū-buntansha) |
YOSHITAKE Tsuyoshi Saitama Medical College Medical Center, Professor, 総合医療センター, 教授 (60010261)
OKA Teruaki Tokyo University Hospital, 医学部・附属病院, 助手 (60177029)
MIYAGI Kagami Tokyo University Hospital, 医学部・附属病院, 助手 (40281703)
TAKEDA Makoto Tokyo University Hospital, 医学部・附属病院, 助手 (10236482)
NAKAJIMA Jun Tokyo University Hospital, 医学部・附属病院, 助手 (90188954)
江連 雅彦 東京大学, 医学部(病), 医員
松本 順 東京大学, 医学部(病), 医員
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| Project Period (FY) |
1993 – 1996
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| Project Status |
Completed (Fiscal Year 1996)
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| Budget Amount *help |
¥32,900,000 (Direct Cost: ¥32,900,000)
Fiscal Year 1996: ¥2,800,000 (Direct Cost: ¥2,800,000)
Fiscal Year 1995: ¥2,800,000 (Direct Cost: ¥2,800,000)
Fiscal Year 1994: ¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 1993: ¥24,100,000 (Direct Cost: ¥24,100,000)
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| Keywords | xenotransplantation / xenoheart transplantation / xenolung transplantation / Japanese monkey / baboon / primates / methotrexate / anti-specific antibody / methotrexate / T細胞 |
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| Research Abstract |
(1) Xeno heart transplantation study
Anti xenograft rejection mechanism was compared with that against allografts by means of heterotopic xeno heart transplantation between Japanese monkeys and baboons. Lymphocyte subset studies in the peripheral blood samples revealed that, even though their behaviors were different between xenografts and allografts, T cell subsets still playd an important role in the concordant xenograft rejection. During the xenograft rejection peripheral blood CD2 (+) cells. CD20 (+) cells CD4 (+) cells and CD8 (+) cells, especially CD2 (+) cells and CD8 (+) cells, increased their numbers.
(2) Xenolung transplantation study
Thirty orthotopic left xenolung transplantations from Japanese monkeys to baboons were performed. The immunosuppression therapy with splenectomy, FK506 and Methotrexate, especially the dose and opportunity of Methotrexate was examined. FK506 alone could not prevent the rejection process against xenolungs. Concomitant use of FK506 and small dose Methotrexate therapy could suppress the cell infiltration into the graft, but could not prevent the anti-specific antibody production. Although our immunosuppressive regimen still need some more revisions, this project revealed that the small dose Methotrexate with FK506 is a feasible combination therapy for xenolung transplantation in primates.
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