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Molecular mechanisms of transduction of gustatory stimuli in human and mammalian tatse cells.

Research Project

Project/Area Number 05404063
Research Category

Grant-in-Aid for General Scientific Research (A)

Allocation TypeSingle-year Grants
Research Field Functional basic dentistry
Research InstitutionNagasaki University

Principal Investigator

SATO Toshihide  Nagasaki University, School of Dentistry, Professor, 歯学部, 教授 (60013968)

Co-Investigator(Kenkyū-buntansha) MATAKI Shiro  Tokyo Medical and Dental University, Faculty of Dentistry, Associate Professor, 歯学部, 助教授 (80157221)
FUJIYAMA Rie  Nagasaki University, School of Dentistry, Research Associate, 歯学部, 助手 (10274664)
MIYAMOTO Takenori  Nagasaki University, School of Dentistry, Research Associate, 歯学部, 助手 (10167679)
OKADA Yukio  Nagasaki University, School of Dentistry, Associate Professor, 歯学部, 助教授 (60136687)
Project Period (FY) 1993 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥18,600,000 (Direct Cost: ¥18,600,000)
Fiscal Year 1995: ¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 1994: ¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 1993: ¥12,700,000 (Direct Cost: ¥12,700,000)
KeywordsTaste Cell / Taste Receptive Membrane / Ion Channel / cAMP / IP_3 / Basolateral Membrane / Four Basic Stimuli / Ca / トランスダクション / 刺激変換 / 受容膜 / アミロライド / チャネル / パッチクランプ / 甘味 / スナネズミ / サイクリックエイエムビ- / アイピ-3 / 味刺激 / カエル
Research Abstract

There are cation and anion channels on the receptive membrane of frog taste cells. Na^+ and Cl^- of NaCl stimuli pass through the cation channel and the anion channel, respectively. Since the Cl^- permeability is lower, a depolarization appears in the taste cell.
We found 80 pS and 40 pS K^+ channels in the frog taste cell membranes. 80 pS K^+ channel in dependent on voltage and Ca^<2+>, but 40 pS K^+ channel is not. Closure of 40 pS K^+ channels is related to cAMP and protein kinase A.Both types of the K^+ channels exist densily on the receptive and dendritic membranes.
Application of amiloride to the receptive membrane of a mouse taste cell within the taste bud produced an outward current, but application of it to the basolateral membrane did not. This suggests that amoloride-sensitive Na^+ channels exist mostly in the receptive membrane.
There are two transduction pathways for sweet stimuli in gerbil taste cells. The gustatory transduction for sugars is concerned with a second messenger cAMP,but that for non-sugars like amino acids is concerned with IP_3.
We have measured internal Ca^<2+> concentration in human taste buds of the fungiform papillae during taste stimulation. Different taste stimuli caused a change in Ca^<2+> concentration of different taste cells in the taste bud, indicating that each taste cell has differential chemical sensitivity.

Report

(4 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • 1993 Annual Research Report
  • Research Products

    (50 results)

All Other

All Publications (50 results)

  • [Publications] Okada,Y.,et al.: "The ionic basis of the receptor potential of frog taste cells induced by water stimuli." J.Exp.Biol.174. 1-17 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Okada,Y.,et al.: "Contribution of proton transportor to acid-induced receptor potential in frog taste cells." Comp.Biochem.Physiol.105A. 725-728 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Okada,Y.,et al.: "Voltage clamping of a frog(Rana catesbeiana)taste cell with a single microelectrode." Comp.Biochem.Physiol.106A. 38-41 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Miyamoto,T.,et al.: "Cationic and anionic channels of apical receptive membrane in a taste cell contribute to generation of salt-induced receptor potential." Comp.Biochem.Physiol.106A. 489-493 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Fujiyama,R.,et al.: "Non-selective cation channel in bullfrog taste cells." NeuroReport. 5. 11-13 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Miyamoto,T.,et al.: "Inhibition of salt-induced gustatory responses in the frog(Rana catesbeiana)by 5'-GMP." Brain Res.629. 345-348 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Sato,T.,et al.: "Receptor potential of the frog taste cell in response to bitter stimuli." Physiol.Behav.56. 1133-1139 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Sato,T.,et al.: "Comparison of gustatory transduction mechanisms in vertebrate taste cells." Zool.Sci.11. 767-780 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Okada,Y.,et al.: "Activation of a cation conductance by acetic acid in taste cells isolated from the bullfrog." J.Exp.Biol.187. 19-32 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Fujiyama,R.,et al.: "Differential distribution of two Ca^<2+>-dependent and -independent K^+ channels throughout receptive and basolateral membranes of bullfrog taste cells." Pflugers Arch.429. 285-290 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Sato,T.,et al.: "Molecular mechanisms of gustatory transduction in frog taste cells." Prog.Neurobiol.46. 239-287 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Sato,T.,et al.: "Quinine-HCl-induced modification of receptor potentials for taste stimuli in frog taste cells." Zool.Sci.12. 45-52 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kurihara,K.,et al.: "Olfaction and Taste XI" Springer-Verlag, 864 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Spielman,A.I.,et al.: "Experimental Cell Biology of Taste and Olfaction" CRL Press, 437 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 小椋 秀亮,他: "歯科医学・歯科医療総論.1.歯科医学総論 2版" 医歯薬出版, 662 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 日本生理学会教育委員会 編: "MCQによる生理学問題集" 文光堂, 331 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Okada, Y., et al.: "The ionic basis of the receptor potential of frog taste cells induced by water stimuli." J.Exp.Biol.174. 1-17 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Okada, Y., et al.: "Contribution of proton transporter to acid-induced receptor potential in frog taste cells." Comp.Biochem.Physiol.105A. 725-728 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Okada, Y., et al.: "Voltage clamping of a frog (Rana catesbeiana) taste cell with a single microelectrode." Comp.Biochem.Physiol.106A. 38-41 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Miyamoto, T., et al.: "Cationic and anionic channels of apical receptive membrane in a taste cell contribute to generation of salt-induced receptor potential." Comp.Biochem.Physiol.106A. 489-493 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Fujiyama, R., et al.: "Non-selective cation channel in bullfrog taste cells." NeuroReport. 5. 11-13 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Miyamoto, T., et al.: "Inhibition of salt-induced gustatory responses in the frog (Rana catesbeiana) by 5'-GMP." Brain Res.629. 345-348 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Sato, T., et al.: "Receptor potential of the frog taste cell in response to bitter stimuli." Physiol.Behav.56. 1133-1139 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Sato, T., et al.: "Comparison of gustatory transduction mechanisms in vertebrate taste cells." Zool.Sci.11. 767-780 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Okada, Y., et al.: "Activation of a cation conductance by acetic acid in taste cells isolated from the bullfrog." J.Exp.Biol.187. 19-32 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Fujiyama, R., et al.: "Differential distribution of two Ca^<2+>-dependent and-independent K^+ channels throughout receptive and basolateral membranes of bullfrog taste cells." Pflugers Arch.429. 285-290 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Sato, T., et al.: "Molecular mechanisms of gustatory transduction in frog taste cells." Prog.Neurobiol.46. 239-287 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Sato, T., et al.: "Quinine-HCl-induced modification of receptor potentials for taste stimuli in frog taste cells." Zool.Sci.12. 45-52 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Sato, T., et al.: In ; Olfaction and Taste XI.Edited by K.Kurihara, et al.Springer-Verlag, Tokyo, 864 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Sato, T., et al.: In ; Experimental Cell Biology of Taste and Olfaction.Edited by Spielman, A.I., et al.CRL Press, Boca Raton, 437 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Okada,Y.,et al.: "The ionic basis of the receptor potential of frog taste cells induced by water stimuli." J.Exp.Biol.174. 1-17 (1993)

    • Related Report
      1995 Annual Research Report
  • [Publications] Fujiyama,R.,et al.: "Non-selective cation channels in bullfrog taste cell membrane." NeuroReport. 5. 11-13 (1993)

    • Related Report
      1995 Annual Research Report
  • [Publications] Sato,T.,et al.: "Comparison of gustatory transduction mechanisms in vertebrate taste cells." Zool.Sci.11. 767-780 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] Fujiyama,R.,et al.: "Differential distribution of two Ca^<2+>-dependent and-independent K^+ channels throughout receptive and basolateral membranes of bullfrog taste cells." Pfiugers Arch.429. 285-290 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] Sato,T.,et al.: "Molecular mechanisms of gustatory transduction in frog taste cells." Prog.Neurobiol.46. 239-287 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Miyamoto,T.,et al.: "Properties of Na^+-dependent K^+ conductance in the apical membrane of frog taste cells." J.Neurosci.Methods. (In press). (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] Kurihara,K.,et al.: "Olfaction and Taste IX" Springer-Verlag, 864 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] Spielman,A.I.,et al.: "Experimental Cell Biology of Taste and Olfaction" CRC, 437 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Sata,O.,et al.: "Electrical responses of non-taste cells in frog tongue and palate to chemical stimuli." Zool.Sci.11. 385-390 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Sato,T.,et al.: "Receptor potential of the frog taste cell in response to bitter stimuli." Rhysiol.Behav.56. 1133-1139 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Sato,T.,et al.: "Comparison of gustatory transduction mechanisms in vertebrate taste cells." Zool.Sci.11. 767-780 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Fujiyama,R.,et al.: "Differential distribution of two Ca^<2+>-dependent and -independent K^+ channels throughout receptive and basolateral membranes bullfrog taste cells." Pflugers Arch.429. 285-290 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Kurihara,K.,et al.: "Olfaction and Taste XI" Springer-Verlag, 864 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 坂田,三弥,他: "基礎歯科生理学 第2版" 医歯薬出版, 428 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Okada,Y.,et al.: "The ionic basis of the receptor potential of frog taste cells induced by water water." J.Exp.Biol.174. 1-17 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Okada,Y.,et al.: "Contribution of proton transporter to acid-induced receptor potential in frog taste cells." Comp.Biochem.Physiol.105A. 725-728 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Okada,Y.,et al.: "Voltage clamping of a frog (Rana catesbeiana)taste cell with a single micro-electrode." Comp.Biochem.Physiol.106A. 37-41 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Miyamoto,T.,et al.: "Cationic and anionic channels of apical receptive membrane in a taste cell contribute to generation of salt-induced receptor potential." Comp.Biochem.Physiol.106A. 489-493 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Fujiyama,R.,et al.: "Non-selective cation channel in bullfrog taste cell membrane." NeuroReport. 5. 11-13 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 長崎大学生命問題研究会: "現代の生命像" 九州大学出版会, 264 (1993)

    • Related Report
      1993 Annual Research Report

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Published: 1993-04-01   Modified: 2016-04-21  

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