• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Establishment of A Novel System for Malignancy Grading of Oral Cancer Using Molecular Biological Technique

Research Project

Project/Area Number 05404069
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Surgical dentistry
Research InstitutionFaculty of Dentistry, Tokyo Medical and Dental University

Principal Investigator

ENOMOTO Shoji  Tokyo Medical and Dental University, Faculty of Dentistry, Professor and Chairman, 歯学部, 教授 (40013940)

Co-Investigator(Kenkyū-buntansha) TACHIKAWA Noriko  Tokyo Medical and Dental University, Faculty of Dentistry, Associate professor, 歯学部, 助手 (70236537)
KAMATA Nobuyuki  Tokyo Medical and Dental University, Faculty of Dentistry, Assistant professor, 歯学部, 助手 (70242211)
TAKAGI Minoru  Tokyo Medical and Dental University, Faculty of Dentistry, Professor and Chairma, 歯学部, 教授 (30014012)
TSUCHIDA Nobuo  Tokyo Medical and Dental University, Faculty of Dentistry, Professor and Chairma, 歯学部, 教授 (60089951)
MUROTA Seiitsu  Tokyo Medical and Dental University, Faculty of Dentistry, Professor and Chairma, 歯学部, 教授 (50072989)
Project Period (FY) 1993 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥20,500,000 (Direct Cost: ¥20,500,000)
Fiscal Year 1996: ¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 1995: ¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 1994: ¥5,800,000 (Direct Cost: ¥5,800,000)
Fiscal Year 1993: ¥8,500,000 (Direct Cost: ¥8,500,000)
Keywordsoral squamous cell carcinoma / normal human keratinocytes from oral mucosa / cell line establishment / vascular endoepitheraial protein / PKC eta / p53 tumor suppressor gene / temperature sensitive mutant / molecular diagnosis / Squamous cell carcinoma / cell line / p53 / cytocaine / cell line. / サイトカイン
Research Abstract

To investigate the difference between tumor cells of the primary site and metastasized tumor cells of oral squamous cell carcinoma (OSCC), we tried to establish cell lines using primary and metastasized tumors independently from a patient. In addition, normal human keratinocytes (NHK) from oral mucosa of the same patient was also subjected to cell line establishment, which served as ideal control cells. In 2 out of 5 cell lines, which were successfully established between 1993-1996, the primary and metastasized tumor cell lines and normal kratinocyte cell lines were simultaneously established in the protein free media.
Transcripts from OSCC and NHK cells were differentially displayd by PCR method using sets of arbitrary primers to search for genes specifically expressed in OSCC or NHK cells.
We reported a protein which was expressed by one of the OSCC cell line and was found to suppress the growth of lymphocytes.
In addition, a monoclonal antibody, which recognize an unknown vascular endo … More epitherial protein, were prepared.
PKC eta is a subtype of the PKC is known to be expressed in the epitherial cells. To understand the cell biological behavior, PKC eta was overexpressed in the epithelial cells using an adenovirul vector. It was found that the treatment by the 12-O-tetradecanoylphorbol 13-acetate (TPA) suppressed the growth of epithelial cells, while fibroblasts were not suppressed under the same condition, suggesting a possible gene therapy of OSCC.
We previously reported that p53 tumor suppressor gene is frequently mutated in OSCC,and is considered to be responsible for the development of this tumor. To know the role of the mutation, the p53 mutants found in OSCC were examined by transfection assays. A mutant with Val of codon 138 were found to be a temperature sensitive mutant. To establish a system of molecular diagnosis using the p53 gene mutations, the status of the gene, clinical process and histological malignancy grade are compared in detail. Although the mutation frequency was so high, we could not found an obvious correlation between the existence of mutation and clinical or histological malignancy grade, suggesting that the p53 mutation is important in the initiation of OSCC. Less

Report

(5 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • 1994 Annual Research Report
  • 1993 Annual Research Report
  • Research Products

    (33 results)

All Other

All Publications (33 results)

  • [Publications] Y.Hirano,et al: "Roles of p53 Mutation in Cell Line Establishment and Identification of the Minimum Transactivation and Transform Suppression Domains" Oral Oncol,Eurf Cancer.31 B.No2. 129-135 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Y.Hirano,et al: "A temperature sensitive mutant of the buman p53 Vall 38 amests rat cell growth without induced expression of cip1/sdi1 after temperature shift down" oncogene. 10. 1879-1885 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 竹内眞樹・土田信夫: "p53の遺伝子変化と生物活性" Surgery Frontier. 2(4). 27-33 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] A.K.Munirajan et al: "p53 Gene Mutations in Oral Careinomas from India" Int. J. Cancer.66. 297-300 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 平野泰正他: "臨床DNA診断法" 金原出版(株), 866頁-868頁 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 鎌田伸之他: "分子生物学研究のための培養細胞実験法5章(4)癌細胞の初代培養10章癌細胞の特性" 羊土社, 12ページ (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Jotte, R., et al.: "Myristylation-dependent transactivation by FBR-v-fos is regulated by C/EBP" JBC. 269. 164-358 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Sugito, K., et al.: "Interaction between hsp70 and hsp40, eukaryotic homologues of Dnak and Dnaj, in human cells expressing mutant-type p53" FEBS Letter. 358. 161-164 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Hirano, Y., et al.: "A temperature sensitive mutant of the p53, Val138, arrests rat cell growth without induced expression of cip1/waf1/sdi1 after temperature shift-down" Oncogene. 10. 1879-1885 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Tsutsumi-Ishii, et al.: "Response of heat shock element within the human HSP70 promoter to mutated p53 genes" Cell Growth & Differenciation. 6. 1-8 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Hirano, Y., et al.: "Roles of p53 mutation in cell line establishment and identification of minimum transactivation and transform suppression domains" Oral Oncol., Eur J Cancer. 31B. 129-135 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Sekiguchi, T.et al.: "Apoptosis is induced in BHK cells by the tsBN462/13 mutation in CCG1/TAFII250 subunit of the TFIID basal cell trascription factor" Experimental Cell Research. 218. 490-498 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yamato, K., et al.: "Temperature-sensitive p53 mutant p53Val-138 : Modulation of the cell cycle, viability and expression of p53-responsive genes" Oncogene. 11. 1-6 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Takada, S., et al.: "Hepatitis B virus X gene expression is activated by X protein but repressed by p53 tumor suppressor product in the transient expression system" Virology. 216. 80-89 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Munirajan, A.K., et al.: "p53 gene mutations in oral carcinomas form India" Int J Cancer. 66. 297-300 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Y.Hirano,et.al: "Roles of p53 mutation in Cell Line Establishement and Identification of the Minimum Transactivation and Transform Suppression Domains" Oral Oncol,Eur J Cencer.31B.No2. 129-135 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] Y.Hirano,et al.: "A temperature sensitive mutant of the human p53,Vall 38,anests rat cell growth without induced expression of cip 1/5di 1 after temperature" Oncogene. 10. 1879-1885 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] 竹内眞樹・土田信夫: "p53の遺伝子変化と生物活性" Surgery Frontier. 2(4). 27-33 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] A.K.Munirajan et al.: "p53 Gene Mutations in Oral Carcinomas from India" Int.J.Cancer. 66. 297-300 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 平野泰正 他: "臨床DNA診断法" 金原出版(株), 866-868 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Hirano Y,Tsutsumi-Ishii Y.and Tsuchida.N.: "Roles of p53 mutation in cell line establishment and identification of the minimum transactivation and transform suppression domins." Oral.Oncol.Eur.J.Cancer. 31B. 129-135 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Hirano.Y.,Yamato K,and Tsuchida N: "A temperature sensitive mutant of the human p53,valine 138 Arrests the growth without induced expression of cip.1-wafinsbi" Oncogine. 10. 1879-1885 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Kenji Yamamoto,Yamamoto M.,Hirano Y.and Tsuchida.N.: "A human temperature-sensitive p53 mutant p53 val-138 Modulation of the cell cycle,viability and expression of p53 resporsir gene." Oncogine. 11. 1-6 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 平野泰正,松村耕治,酒井英紀,土田信夫: "口腔癌-臨床DNA診断" 金原出版, 3- (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 鎌田伸之他: "分子生物研究のための培養細胞実験法 5章(4)癌細胞の初代培養 10章癌細胞の特性" 羊土社, (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Hirano Y Tsutsumi-Ishii.Y and Tsuchida.N.: "Roles of P53 mutation in cell line establishment and identification of the minimum trahsactivation and transform suppression domains" Eur.J.of Cancer. (in perss).

    • Related Report
      1994 Annual Research Report
  • [Publications] Hirano.Y.,Yamatok,and Tsuchida N: "A temperature sensitive mutant of the human P53,Valiue 138 Arrests the growth without induced expression of cip・1-Wafz-sbi" Oncogine. (in perss).

    • Related Report
      1994 Annual Research Report
  • [Publications] 平野泰正,松村耕治,酒井英紀,土田信夫: "口腔癌-臨床DNA診断" 金原出版, (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] 鎌田伸之他: "分子生物学研究のための培養細胞実験法5章(4)癌細胞の初代培養 10章癌細胞の特性" 羊土社, 243 (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] 塗谷達ら他3名: "口腔粘膜疾患の分子生物学的研究 第1報.口腔粘膜扁平苔癬における IL-8の発現" 日本口腔科学会雑誌. 42. 1108 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 戸田ひとみ 他4名: "口腔癌の産生する血管内皮細胞増殖抑制因子とそのモノクローナル抗体の作製" 日本口腔科学会雑誌. 42. 922 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 藤井万紀子 他3名: "口腔扁平上皮癌が産生する免疫抑制活性について" 日本口腔科学会雑誌. 42. 921 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 平野泰正 他4名: "口腔癌と癌抑制遺伝子 P53" 口腔病学会誌. 60. 216 (1993)

    • Related Report
      1993 Annual Research Report

URL: 

Published: 1993-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi