| Project/Area Number |
05404073
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| Research Category |
Grant-in-Aid for General Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
医薬分子機能学
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| Research Institution | HOKKAIDO UNIVERSITY |
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Principal Investigator |
OHTSUKA Eiko Hokkaido University, Fac.Pharm.Sci., PROFESSOR., 薬学部, 教授 (80028836)
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| Co-Investigator(Kenkyū-buntansha) |
NIKAIDO Osamu Kanazawa University, Fac.Pharm.Sci., PROFESSOR., 薬学部, 教授 (60019669)
MORIOKA Hiroshi Hokkaido University, Fac.Pharm.Sci., INSTRUCTORS., 薬学部, 助手 (20230097)
IWAI Shigenori Hokkaido University, Fac.Pharm.Sci., INSTRUCTORS., 薬学部, 助手 (10168544)
INOUE Hideo Hokkaido University, Fac.Pharm.Sci., ASSOCIATE PROFESSOR., 薬学部, 助教授 (80088856)
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| Project Period (FY) |
1993 – 1994
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| Project Status |
Completed (Fiscal Year 1994)
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| Budget Amount *help |
¥25,200,000 (Direct Cost: ¥25,200,000)
Fiscal Year 1994: ¥3,900,000 (Direct Cost: ¥3,900,000)
Fiscal Year 1993: ¥21,300,000 (Direct Cost: ¥21,300,000)
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| Keywords | UV-damaged DNA / Thymine Dimer / PCR Cloning / Single Chain Antibody / Flexible Linker |
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| Research Abstract |
Two types of cDNAs encoding mouse immauoglobulin heavy (VH) and light (Vc) chain variable (V) domains against UV-induced DNA damage have been prepared. The cDNA was cloned for seyuencing and expression, and the nucleoticle sequences of the V domains were determined by PCR.Genes for a linker (Gly_4-Ser)_3 was inserted between the two domains, and the gene was expressed using the T7 promoter.
The single chain Fv for pyrimidine (6-4) photo product was purified and tested for its ability to oligonucleotides containing the (6-4) photoproduct. The scFv was found to be specific.
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