| Project/Area Number |
05453110
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
生物・生体工学
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| Research Institution | Tokyo Institute of Technology |
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Principal Investigator |
AIZAWA Masuo (1994) Tokyo Inst.of Tech., Dept.of Bioengine.Proffesor, 生命理工学部, 教授 (00016742)
碇山 義人 (1993) 東京工業大学, 生命理工学部, 助教授 (10202847)
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| Co-Investigator(Kenkyū-buntansha) |
KOBATAKE Eiry Tokyo Inst.of Tech., Dept.of Bioengine.Lecture, 生命理工学部, 講師 (00225484)
相澤 益男 東京工業大学, 生命理工学部, 教授 (00016742)
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| Project Period (FY) |
1993 – 1994
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| Project Status |
Completed (Fiscal Year 1994)
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| Budget Amount *help |
¥6,700,000 (Direct Cost: ¥6,700,000)
Fiscal Year 1994: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 1993: ¥5,100,000 (Direct Cost: ¥5,100,000)
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| Keywords | Cell free protein production / Reactor / Protein A / Luciferase / セル・フリータンパク質 / 固定化mRNA / タンパク質生産 / 生体外タンパク質合成 / バイオリアクター |
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| Research Abstract |
In the first year of the project, protein A was produced by a cell free protein synthesis system which we developed. An important characteristic of our system is that a mRNA is immobilized on the beads through the end of mRNA chain. In the result, it was proved that such the immobilized mRNA has a high resistance for mRNA degradation enzymes.
In the last year of the project, we investigated the repetition use of immobilized mRNA on the production of luciferase. Production profile of the luciferase system is able to observed by monitoring the luminescence of produced luciferase.
In conclusion, unstability of mRNA in production cell can be largely overcome by the immobilization of mRNA.All the results of our project suggest that the cell free protein production system could utilized for commercially protein production.
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