| Project/Area Number |
05453122
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
工業物理化学
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| Research Institution | Osaka University |
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Principal Investigator |
KAI Yasushi Osaka University Fuculty of Engineering Professor, 工学部, 教授 (40029236)
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| Co-Investigator(Kenkyū-buntansha) |
INOUE Tsuyoshi Osaka University Fuculty of Engineering Research Assistant, 工学部, 助手 (20263204)
KANEHISA Nobuko Osaka University Fuculty of Engineering Research Assistant, 工学部, 助手 (20177538)
原田 繁春 大阪大学, 工学部, 講師 (80156504)
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| Project Period (FY) |
1993 – 1994
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| Project Status |
Completed (Fiscal Year 1994)
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| Budget Amount *help |
¥5,000,000 (Direct Cost: ¥5,000,000)
Fiscal Year 1994: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1993: ¥3,900,000 (Direct Cost: ¥3,900,000)
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| Keywords | X-ray structure analysis / CO_2-fixation enzymes / spinach / 2.0 A resolution / RuBisCo / phosphoenolpyrubicacidcarboxylase (PEPC) / reflection-intensity-measurement / 二酸化炭素固定 / リブロースビスリン酸カルボキシラーゼ / 結晶構造解析 |
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| Research Abstract |
The spinach RuBisCO (ribulose-1,5-bisphosphate carboxylase oxygenase) were purified and crystallized by the use of polyethylene glycol as a salt for salting out during the purification process and a precipitant for crystallization. The X-ray diffraction data were measured by the use of synchrotron radiation in Tsukuba KEK.The refinement of the crystal structure was proceeded up to the current R value of 19.0% for 2.0 ? 81 resolution data. The comparison of the molecular structure obtained by the present work with the spinach RuBisCO obtained by use of ammonium sulfate as a salt and precipitant are under investigation. The difference in the enzymatic function between these RuBisCO is expected to understand from the structure-function correlation based on the high resolution molecular structures.
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