Synthesis of molecular sensors for in situ use
| Project/Area Number |
05453134
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Synthetic chemistry
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| Research Institution | TOHOKU UNIVERSITY |
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Principal Investigator |
ANZAI Jun-ichi Pharm. Inst., Tohoku Univ., Asoc. Prof., 薬学部, 助教授 (40159520)
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| Co-Investigator(Kenkyū-buntansha) |
SUZUKI Iwao Pharm. Inst., TohokuUniv., Instructor, 薬学部, 助手 (30226493)
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| Project Period (FY) |
1993 – 1995
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| Project Status |
Completed (Fiscal Year 1995)
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| Budget Amount *help |
¥7,000,000 (Direct Cost: ¥7,000,000)
Fiscal Year 1995: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1994: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1993: ¥3,400,000 (Direct Cost: ¥3,400,000)
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| Keywords | Molecular recognition / Surface Modification / Cyclodextrin / Crown ether / LB membrane / Avidin / 分子認識素子 / 表面 / テオール / 電気化学 / 計測 |
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| Research Abstract |
1) Synthesis of cyclodextrin (CD) derivatives and surface modification ; CD monolayrs were prepared on electrode surface using lipoic acid-modified CDs. Ferrocene can penetrate into the CD monolayr membrane, and the permeability can be regulated by the addition of the other guest compounds. CD derivatives which are substituted with lipoic acid and ferrocene simultaneously were also prepared. Self-assembled membranes are now being prepared based on the di-substituted CDs.
2) Synthesis of photosensitive lipids and surface modification ; Artificial lipids containing photosensitive azobenzene residues were prepared and their photoresponse in LB membrane was estimated. Photoisomerization reactions of the azobenzene lipids bearing double alkyl chains were superior to those of the single alkyl chain-substituted lipids. Crown ether-substituted azobenzene lipids were also synthesized and ion sensitivity and photoresponse of the LB membranes were studied. The crown ether-substituted lipids exhibited a selective ion binding behavior and photoresponse in the LB membrane.
3) Surface modification based on selective binding protein ; Avidin was found to form monomolecular layr on the surface of metal electrodes. Monomolecular layrs of biotin-labeled enzyme can be prepared on the surface of the avidin-modified electrodes.
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Report
(4 results)
Research Products
(15 results)
