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Preparation of affinity latices and their application t of bioseparaton and cell activation.

Research Project

Project/Area Number 05453214
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Biomedical engineering/Biological material science
Research InstitutionKeio University

Principal Investigator

KAWAGUCHI Haruma  Keio University Professor Dept.of Appl.Chem., 理工学部, 教授 (30051808)

Co-Investigator(Kenkyū-buntansha) FUJIMOTO Keiji  Keio University Assistant Dept.of Appl.Chem., 理工学部, 助手 (70229045)
Project Period (FY) 1993 – 1994
Project Status Completed (Fiscal Year 1994)
Budget Amount *help
¥6,200,000 (Direct Cost: ¥6,200,000)
Fiscal Year 1994: ¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 1993: ¥3,200,000 (Direct Cost: ¥3,200,000)
KeywordsAffinity latex / Bioseparator / Cell activator / Transcription factor / Membrane protein / Microsphere / ミクロスフェア / アフィニティ / ラテックス / スペーサー / DNA / RNA / RGDS / 転写制御 / ホルモン
Research Abstract

Either of biospecific couple was immobilizud to latex particles which were hydrophilic and have binding sites for the immobilization. Affinity latices thus prepared were employed for separation/purification of complementaey component, or cell activation via specific binding reaction. The following couples were used in this study ;
a. (DNA) n E4TF1
b.TFIID TFIIA
c.Subunit of E4TF1 small nuclear Ribonucleo-Protein
d.DNA Complementary DNA
e.senseDNA antisenseRNA
f.RGDS GPIIb/GPIIIa
In terms of f, RGDS-carrying affinity latex was used for two purposes ; GPIIb/IIIa purification from platelet membrane extract and leukocyte activation through RGD-GPIIb/IIIa interaction transferred to actin filament.
The following three processed must be developed to get efficient affinity latices ; 1.Preparation of suitable carrier particle, 2.Immobilization of biocompounds in a proper mode, and 3.Search of suitable conditions to exhibit efficient biospecific reaction on particle surface.The effect of physical properties of particles on the efficiency of affinity latex was studied in detail.Polystirene-core/poly (qlycidyl methacrylate) shell particles were especially excellent among particles examined.In some systems, subunits or fragments of biofunctional molecules were immobilized on the particles.The resulting hybrid particles exhibited sufficient ability in bioseparation.Introduction of spacer chains between biocompound and particle surface improved the activity.

Report

(3 results)
  • 1994 Annual Research Report   Final Research Report Summary
  • 1993 Annual Research Report
  • Research Products

    (24 results)

All Other

All Publications (24 results)

  • [Publications] Inomata,Y.: "Preparation of DNA-carrying affinity latex and purification of transcription factors with the latex." J.Biomat.Sci.Polym.Ed.5. 293-302 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kasuya,Y.: "Activation of human neutrophils by Arg-Gly-Asp-Ser immmobilized on microspheres." J.Biomed.Mat.Res.28. 397-404 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Sumi,Y.: "Application of cationic latex particles for protain separation." Colloids and Surfaces B:Biointerfaces.2. 419-427 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kasuya,Y.: "Influence of the structure of support microspheres on leucocyte activation by RGDS-carrying microspheres." Biomaterials. 15. 944-949 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Urakami,Y.: "Phagocytosis of microspheres with modified surfaces." Colloids and Surfaces B:Biointerfaces.3. 183-190 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Inomata,Y.: "Purification of membrane receptors with pepitide-carrying affinity latex particles." Colloids and Surfaces B:Biointerfaces.(Accepted for publication).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Inomata, Y., et al.: "Preparation of DNA-carrying affinity latex and purification of transcription factors with the latex." J.Biomat.Sci Pplym.Ed.5. 293-302 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kasuya, Y., et al.: "Activation of human neutrophils by Arg-Gly-Asp-Ser immmobilized on microspheres." J.Biomed.Mat.Res.28. 397-404 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Sumi, Y., et al.: "Application of cationic latex particles for protain separation." Colloids and Surfaces B : Biointerfaces.2. 419-427 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kasuya, Y., et al.: "Influence of the structure of support microspheres on leucocyte activation by RGDS-carrying microspheres." Biomaterials. 15. 944-949 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Urakami, Y., et al.: "Phagocytosis of microspheres with modified surfaces." Colloids and Surfaces B : Biointerfaces.3. 183-190 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Inomata, Y., et al.: "Purification of membrane receptors with pepitide-carrying affinity latex particles." Colloids and Surfaces B : Biointerfaces.(Accepted for publication).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Inomata,Y.: "Preparation of DNA-carrying affinity latex and purification of transcription factors with the latex." J.Biomat.Sci.Polym.Ed.5. 293-302 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Kasuya,Y.: "Activation of human neutrophils by Arg-Gly-Asp-Ser immmobilized on microspheres." J.Biomed.Mat.Res.28. 397-404 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Sumi,Y.: "Application of cationic latex particles for protain separation." Colloids and Surfaces B:Biointerfaces.2. 419-427 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Kasuya,Y.: "Influence of the structure of support microspheres on leucocyte activation by RGDS-carrying microspheres." Biomaterials. 15. 944-949 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Urakami,Y.: "Phagocytosis of microspheres with modified surfaces." Colloids and Surfaces B:Biointerfaces.3. 183-190 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Inomata,Y.: "Purification of membrane receptors with pepitide-carrying affinity latex particles." Colloids and Surfaces B:Biointerface.(Accepted for publication).

    • Related Report
      1994 Annual Research Report
  • [Publications] K.Fujimoto: "Activation ofLeukocytes by RGDS-carrying Microspheres" ACS Symposium Series. 540. 220-227 (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] Y.Kasuya: "Activation of Human Neutrophil by Arg-Gly-Asp-Ser Immobilized on Microspheres" J.Biomed.Mater.Res.28. 397-404 (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] Y.Kasuya: "Influence of the Structure of the Support Microspheres on Leucocytes Activation by RGDS-carrying Microspheres" Biomaterials. (accepted).

    • Related Report
      1993 Annual Research Report
  • [Publications] Y.Inomata: "Preparation of DNA-carrying Affinity Latex and Purification of Transcription Factors with the Latex" J.Biomater.Sci.,Polym.Ed.(in press).

    • Related Report
      1993 Annual Research Report
  • [Publications] Y.Sumi: "Application of Cationic Latex Particles for Protein Separation" Colloids and Surfaces. (in press).

    • Related Report
      1993 Annual Research Report
  • [Publications] H.Kawaguchi: "Bilomedical Applications of Polymeric Materials" CRC Press, 26 (1993)

    • Related Report
      1993 Annual Research Report

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Published: 1993-04-01   Modified: 2016-04-21  

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