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Molecular interaction between Agrobacterium and plant cells

Research Project

Project/Area Number 05454017
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 植物生理
Research InstitutionNagoya University

Principal Investigator

MACHIDA Yasunori  Faculty of Science, Nagoya University Biology, Professor, 理学部, 教授 (80175596)

Co-Investigator(Kenkyū-buntansha) YOSHIOKA Yasushi  Faculty of Science, Biology, Research associate, 農学部, 助手 (60202397)
Project Period (FY) 1993 – 1994
Project Status Completed (Fiscal Year 1994)
Budget Amount *help
¥6,700,000 (Direct Cost: ¥6,700,000)
Fiscal Year 1994: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1993: ¥5,000,000 (Direct Cost: ¥5,000,000)
KeywordsAgrobacterium / Ti plasmid / protein kinase / vir genes / phenol / monosaccharides
Research Abstract

Crown gall tumor is incited on dicotyledonous plants by infection with Agrobacterium tumefaciens harboring Ti plasmid. Upon infection, expression of most of vir genes on the Ti plasmid is induced or enhanced in the bacterial cells.Some of the vir gene products are involved in transfer of T-DNA which is transferred. We have previously found that monosaccharides such as glucose and galactose can enhance signals initiated by plant phenolic compounds. In the present study, we demonstrated that the signal by such monosaccharides is transduced into cytoplasm through the sensor protein VirA.In addition, the periplasmic domain of VirA was shown to be responsible for signal perception by the sugars and the linker domain and the region of the C-terminal side of the TM2 domain are shown to be involved in signaling of phenolics. In addition to these investigations, we have examined whether infection of plants with Agrobacterium cells activates a certain protein kinase. Our results showed that a novel protein kinase which has molecular mass of 46 kDa was transiently activated by infection of leaf discs of tobacco. Activities of 46-kDa kinases were detected in a wide variety of plant species after wounding, indicating that this protein kinase is present in a number of plants. It should be important to investigate physiological relationship between infection with Agrobacterium and function of this kinase.

Report

(3 results)
  • 1994 Annual Research Report   Final Research Report Summary
  • 1993 Annual Research Report
  • Research Products

    (23 results)

All Other

All Publications (23 results)

  • [Publications] Toshiya Muranaka: "A model for a bioconversion system with the promoter of partAt gene which confers a high level of expression of a transgene in hairy roots" Applied Microbiology Biotechnology. 40. 841-845 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Toshiya Muranaka: "Characterization of tobacco protein kinase NPK5,a homolog of Saccharomyces cerevisiae SNF1 that constitutively activates expression of the glucose-repressible SUC2gene for a secreted invertase of S.cerevisiae" Molecular and Cellular Biology. 14. 2958-2965 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Yasuko Niwa: "Organ-specific and auxin-inducible expression of two tobacco parA-related genes in transgenic plants" DNA RESEARCH. 1. 213-221 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Yukihiro Ito: "NPK15,a tobacco protein-serine/threonine kinase with a single hydrophobic region near the amino-terminus" Mo1.Gen.Genet.245. 1-10 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Toshiharu Ohba: "DNA rearrangement associated with the integration of T-DNA in tobacco:an example for multiple duplication of DNA around the integration target" The Plant Journal. 7. 157-164 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Wataru Shibata: "A tobacco protein kinase,NPK2,has a domain homologous to a domain found in activators of mitogen^activated protein kinase(MAPKKs)" Mo1.Gen.Genet.246(in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Toshiya Muranaka: "A model for a bioconversion system with the promoter of the parAt gene which confers a high level of expression of a transgene in hairy roots" Applied Microbiology Biotechnology. 40. 841-845 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Toshiya Muranaka: "Characterization of tobacco protein kinase NPK5, a homolog of Saccharomyces cerevisiae SNFI that constitutively activates expression of the glucose-repressible SUC2 gene for a secreted invrrtase of S.cerevisiae" Molecular and Cellular Biology. 14. 2958-2965 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Yasuo Niwa: "Organ-specific and auxin-inducible expression of two tobacco parA-related genes in transgenic plants" DNA RESEARCH. 1. 213-221 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Yukihiro Ito: "NPK15, a tobacco protien-serine/threonine kinase with a single hydrophobic region near the amino-terminus" Mol.Gen.Genet.245. 1-10 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Toshiharu Ohba: "DNA rearrangement associated with the integtation of T-DNA in tobacco : an example for multiple duplication of DNA around the intergration target" The Plant Journal. 7. 157-164 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Wataru Shibata: "A tobacco protein kinase, NPK2, has a domain homologous to a domain found in activators of mitogen-activated protein kinase (MAPKKs)" Mol.Gen.Genet.(in press). 246

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Toshiya Muranaka: "A model for a bioconversion system with the promoter of the parAt gene which c0nfers a high level of expression of a transgene in hairy roots" Applied Microbiology Biotechnology. 40. 841-845 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Toshiya Muranaka: "Characterization of tobacco protein kinase NPK5,a homolog of Saccharomyces cerevisiae SNF1 that constitutively activates expression of the glucose‐repressible SUC2 gene for a secreted invertase of S.cerevisiae" Molecular and Cellular Biology. 14. 2958-2965 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Yasuo Niwa: "Organ‐specific and auxin‐inducible expression of two tobacco parA‐related genes in transgenic plants" DNA RESEARCH. 1. 213-221 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Yukihiro Ito: "NPK15,a tobacco protien‐serine/threonine kinase with a single hydrophobic region near the amino‐terminus" Mol.Gen.Genet.245. 1-10 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Toshiharu Ohba: "DNA rearrangement assosiated with the integration of T‐DNA in tobacco:an example for multiple duplication of DNA around the integration target" The Plant Journal. 7. 157-164 (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] Wataru Shibata: "A tobacco protein kinase,NPK2,has a domain homologous to a domain found in activators of mitogen‐activated protein kinase(MAPKKs)" Mol.Gen.Genet.246(in press).

    • Related Report
      1994 Annual Research Report
  • [Publications] Yasunori Machida: "Molecular interactions between Agrobacterium and Plant cells" the symposium book of 6th ISMP(ed.by E.W.Nester)Kluwer. 85-96 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Yasushi Yoshioka: "Mechanisms of T-DNA transfer and integration into plant chromosomes:role of virB,virD4 and virE2 and a short interspersed repetitive element(SINE)from tobacco" Molecular Mechanisms of Bacterial Virulence. (in press). (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] Yasushi Yoshioka: "Molecular characterization of a short interspersed repetitive element form tobacco that exhibits sequence homology to specific tRNAs" Proc.Natl.Acad.Sci.USA. 90. 6562-6566 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Nobuyoshi Shimoda: "Genetic Evidence for an Interaction between the VirA Sensor Protein and the ChvE Sugar-binding Protein of Agrobacterium" The Journal of Biological Chemistry. 268. 26552-26558 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Toshiya Muranaka: "A model for a bioconversion system with the promoter of the parAt gene,which confers a high level of expression of a transgene in hairy roots" Applied Microbiology Biotechnology. (in press). (1994)

    • Related Report
      1993 Annual Research Report

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Published: 1993-04-01   Modified: 2016-04-21  

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