| Project/Area Number |
05454021
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
生物形態・構造
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| Research Institution | University of Tokyo |
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Principal Investigator |
KAWASHIMA Seiichiro Univ, of Tokyo, Grad.Sch of Sci.Professor, 大学院・理学系研究科, 教授 (20011554)
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| Co-Investigator(Kenkyū-buntansha) |
OKA Yoshitaka Univ, of Tokyo, Grad.Sch of Sci.Assistant, 大学院・理学系研究科, 助手 (70143360)
PARK Min kyun Univ, of Tokyo, Grad.Sch of Sci.Assor, Professor, 大学院・理学系研究科, 助教授 (00228694)
MORI Takao Univ, of Tokyo, Grad.Sch of Sci.Professor, 大学院・理学系研究科, 教授 (80011659)
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| Project Period (FY) |
1993 – 1994
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| Project Status |
Completed (Fiscal Year 1994)
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| Budget Amount *help |
¥6,400,000 (Direct Cost: ¥6,400,000)
Fiscal Year 1994: ¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1993: ¥4,200,000 (Direct Cost: ¥4,200,000)
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| Keywords | Hormone receptor / cDNA cloning / RT-PCR reaction / Hypothalamus / Pituitary / Gonad / Comparative endocrinology / In situ hybridization / 転写制御 |
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| Research Abstract |
This project aims to clarify the regulatory mechanism of peptide hormone receptors relating reproductive function. The results obtained are as follows.
(1)The whole base sequence of cDNA encoding gonadotropin-releasing hormone (GnRH) was analyzed. The expression of mRNA for GnRH receptors in the pituitary is found to be limited to LH and FSH cells. Furthermore, the expression in the ovarian follicles both maturing and atreticindicates the role of GnRH in follicular maturation and atresia.
(2)Gonadotropin receptor cDNAs were analyzed in various animal species. Sequencing of extracellular domain was completed in the quail, first discovery in avian species. The determination of epitopes of raised antibody for the extracellular domain was partly completed.
Regulation of receptor number by artificial manipulation of photoperiod in wild birds showed the results as expected.
(3)Radioreceptor assay revealed that the uterus contains specific prolactin receptors preferentially in the myometrial cells. The prolactin binding capacity of the uterus was elevated by prolactin and tended to increase by estrogen.
(4)Immunohistochemical study in dwarf gourami revealed that some GnRH neurons showed isotocin immunoreactivity, indicating functional correlation between GnRH and isotocin.
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